Differential Susceptibility of<i>Malus</i>spp. Cultivars Robusta 5, Novole, and Ottawa 523 to<i>Erwinia amylovora</i>

Norelli, John L.; Aldwinckle, H. S.; Beer, Steven V.

doi:10.1094/pd-70-1017

Cited by 47 publications

(27 citation statements)

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“…Most importantly, it acts as a signaling molecule inducing programmed cell death of the host plant (Greenberg 1996). Norelli et al (1986) examined E. amylovora strains and found that they showed different virulence for different cultivars of apple trees. On the other hand, Schwartz et al (1991) showed that only some strains of this bacterium inhibited the growth of pear cells in agar medium.…”

Section: Host Range and Virulencementioning

confidence: 99%

Phenotypic and genetic diversity of Erwinia amylovora: the causal agent of fire blight

Puławska

Sobiczewski

2011

Trees

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Erwinia amylovora is a polyphagous bacterium causing fire blight on apple, pear and over 130 other plant species belonging mainly to the Rosaceae family. Although E. amylovora is regarded as a very homogenous species, the particular strains can differ in pathogenic ability as far as their host range is concerned (e.g. those originating from Rubus or Maloidae plants) as well as by the extent of the disease they cause. It was found that strains originating from North America are generally more genetically heterogeneous than those from Europe. Diversity of E. amylovora is also related to streptomycin resistance as a result of its application to control of fire blight. The level of genetic heterogeneity of E. amylovora is so low (comparative genome analysis revealed a similarity of over 99% for the two genomes tested) that standard DNA-based techniques fail in detection of intra-species variability. Amplified fragment length polymorphism was found to be most useful for differentiation of strains of fire blight causal agent as well as techniques ensuing release of pangenome sequences of two E. amylovora strains: multi-locus variable number of tandem repeats analysis and clustered regularly interspaced short palindrome repeats.

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Section: Host Range and Virulencementioning

confidence: 99%

Phenotypic and genetic diversity of Erwinia amylovora: the causal agent of fire blight

Puławska

Sobiczewski

2011

Trees

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“…The shoots (Type 1 and Type 2 plants) were inoculated using bacterial suspension of 10 8 cfu ml -1 by hypodermic injection method (Norelli et al 1986). The treated shoots were labeled with flagging tape for evaluation purposes.…”

Section: Experimental Design and Setupmentioning

confidence: 99%

Determination of application time for chemical control of fire blight disease in pear varieties

MADEN¹

2010

Tarım Bilimleri Dergisi

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Fire blight, caused by the bacterium Erwinia amylovora, is a serious disease of pear, apple, and other plants of the Rosaceae family. In this study, from the point of view of continuousness of protection of fire blight disease and shoot growth in growing season, application times and effectiveness of host resistance inducers, harpin protein, benzothiadiazole, prohexadione-Ca as alternatives to conventional products, streptomycin, copper and maneb+copper were evaluated on susceptible pear varieties in greenhouse and field conditions. Type 1 and Type 2 applications for prevention of the disease and Type 3 and Type 4 applications for evaluation of shoot growth were performed. Type 2 application of harpin protein gave remarkable effectiveness on prevention of the disease about 49% and 65% in greenhouse and field, respectively. After Type 1 and 2 applications by prohexadione-Ca and benzothiadiazole, disease severity significantly decreased comparing to applications of copper and maneb+copper and, controls. Only prohexadione-Ca applications significantly reduced shoot lengths and plants were highly affected by the application Type 4 of this chemical. According to findings, applications of Type 2 provided better results than Type 1 on all of pear varieties in greenhouse and field conditions and use of resistance inducing substances during the production season is proposed in managing of shoot blight phase of fire blight disease. 151uygulaması hastalığın önlenmesinde sırasıyla sera ve arazide %49 ve %65 oranlarında dikkate değer etkililik vermiştir. Prohexadione-Ca ve benzothiadiazole ile Tip 1 ve Tip 2 uygulamalarından sonra, hastalık şiddeti bakır, maneb+bakır uygulamalarına ve kontrollere kıyasla önemli ölçüde azalmıştır. Sürgün uzunluklarını sadece prohexadione-Ca uygulamaları önemli olarak azaltmış ve bitkiler, bu kimyasalın Tip 4 uygulamasından büyük ölçüde etkilenmişlerdir. Elde edilen bulgulara göre, tüm armut çeşitlerinde, sera ve arazi koşullarında Tip 2 uygulamaları, Tip 1'den daha iyi sonuçlar sağlamıştır ve ateş yanıklığı hastalığının sürgün yanıklığı evresi kontrolünde, dayanıklılığı teşvik eden kimyasalların kullanımı önerilmektedir.

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“…In addition, the presence of different plasmids was found to be the source of genetic diversity (McGhee and Jones 2000; Llop et al 2008;Ismail et al 2014). Although E. amylovora strains are very homogeneous in terms of phenotypic and genetic features, they show variations in pathogenic ability (Cabrefiga and Montesinos 2005;Hevesi et al 2000;Puławska et al 2006;Sholberg et al 2001;Norelli et al 1986).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of different RNA extraction methods for high-quality total RNA and mRNA from Erwinia amylovora in planta

et al. 2016

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Obtaining a sufficient quantity of high-quality, intact RNA is the first crucial step in its study by RNA sequencing on next-generation sequencing platforms or quantitative PCR. Different RNA extraction methods or commercial kits vary in yield and in the quality and integrity of the RNA obtained, which may affect the results of downstream applications. Often, these factors depend on the organism under study and nature of the sample. Therefore, the selection of an appropriate RNA isolation method is critical. In this study, we present the results of an evaluation of three different commercial kits for the isolation of total RNA from Erwinia amylovora in apple tissue as well as the usefulness of different kinds of Deoxyribonuclease I for DNA removal and kits for rRNA depletion. To our knowledge, this is the first report on the method of isolation of high-quality E.amylovora mRNA for RNA-seq.

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Differential Susceptibility ofMalusspp. Cultivars Robusta 5, Novole, and Ottawa 523 toErwinia amylovora

Cited by 47 publications

References 0 publications

Phenotypic and genetic diversity of Erwinia amylovora: the causal agent of fire blight

Phenotypic and genetic diversity of Erwinia amylovora: the causal agent of fire blight

Determination of application time for chemical control of fire blight disease in pear varieties

Evaluation of different RNA extraction methods for high-quality total RNA and mRNA from Erwinia amylovora in planta

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