Differentially expressed mammary proteins during lactation in dairy sheep

Signorelli, Federica; Cifuni, Giulia Francesca; Miarelli, M.

doi:10.1016/j.livsci.2012.07.018

Cited by 8 publications

(7 citation statements)

References 38 publications

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“…Staphylococcal nuclease domain-containing protein 1 was increased in humans in response to lactogenic hormones . Apolipoprotein A-I observed in this study’s PM fractions has been involved in delivering cholesterol to milk fat globules, and the highest expression of this protein occurred at midlactation . The eukaryotic translation elongation factor 2 was also present in the PM fractions.…”

Section: Discussionmentioning

confidence: 53%

“…The eukaryotic translation elongation factor 2 was also present in the PM fractions. It has been reported that the main limiting factor in the protein synthesis at the end of lactation was the availability of this protein. , The enzymes of the pentose phosphate pathway (transketolase), the tricarboxylic acid cycle (cytosolic NADP-isocitrate, succinate dehydrogenase, and malate dehydrogenase), and de novo lipogenesis (fatty acid synthase and fatty acid-binding proteins) were also identified in the PM fractions. These proteins provide a substantial fraction of the reducing equivalents of NADPH and long-chain fatty acids taken up from blood, needed for lipid synthesis in the mammary gland. − …”

Section: Discussionmentioning

confidence: 99%

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Proteomic Analysis of Isolated Plasma Membrane Fractions from the Mammary Gland in Lactating Cows

Yan

Tang

Tan

et al. 2015

J. Agric. Food Chem.

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The mammary gland of dairy cows is a formidable lipid-synthesizing machine for lactation. This unique function depends on the activities of plasma membrane (PM) proteins in mammary cells. Little information is known about the expression profiles of PM proteins and their functions during the lactating process. This study investigated the proteome map of PM fractions of mammary gland in lactating cows using 1D-Gel-LC-MS/MS and identified 872 nonredundant proteins with 141 unknown proteins, wherein 215 were PM-associated proteins. Most of the PM-associated proteins were binding, transport, and catalytic proteins such as annexin proteins, heat shock proteins, integrins, RAS oncogene family members, and S100 calcium binding proteins. The PM-associated pathways such as caveolae-mediated endocytosis, leukocyte extravasation, aldosterone signaling in epithelial cells, and remodeling of epithelial adherens junctions were also significantly over-represented. Proteomic analysis revealed the characteristics and predicted functions of PM proteins isolated from the lactating bovine mammary gland. These results further provide experimental evidence for the presence of many proteins predicted in the annotated bovine genome. The data generated here also provide a reference for the PM-related functional research in the mammary gland of lactating cows.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Isolated Plasma Membrane Fractions from the Mammary Gland in Lactating Cows

Yan

Tang

Tan

et al. 2015

J. Agric. Food Chem.

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“…Different researchers have worked on varying fractions of milk. Relevant works include the study of protein synthesis during lactation [27], the comparison of ewes’ milk with that of cows [28] and the study of proteins in the milk of indigenous sheep breeds [6,29]. Further studies have investigated and elucidated enzymes involved in milk synthesis and the formation of milk lipids [30,31], as well as the metabolism of glucose or lactose during lactation [32].…”

Section: Use Of Proteomics In the Study Of Milk Production In Healmentioning

confidence: 99%

Use of Proteomics in the Study of Mastitis in Ewes

2019

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The objective of this review is to describe the usage and applicability of proteomics technologies in the study of mastitis in ewes. In ewes, proteomics technologies have been employed for furthering knowledge in mastitis caused by various agents (Staphylococcus aureus, Staphylococcus chromogenes, Mannheimia haemolytica, Streptococcus uberis, Mycoplasma agalactiae). Studies have focused on improving knowledge regarding pathogenesis of the infections and identifying biomarkers for its diagnosis. Findings have revealed that ewes with mastitis mount a defence response, controlled by many proteins and over various mechanisms and pathways, which are interdependent at various points. Many proteins can participate in this process. Moreover, as the result of proteomics studies, cathelicidins and serum amyloid A have been identified as proteins that can be used as biomarkers for improved diagnosis of the disease. In the long term, proteomics will contribute to improvements in the elucidation of the pathogenesis of mastitis. Further in-depth investigations into the various proteomes and application of new methodological strategies in experimental and clinical studies will provide information about mastitis processes, which will be of benefit in controlling the disease. Improvement of diagnostic techniques, establishment of prognostic tools and development of vaccines are key areas for targeted research.

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“…In sheep, Signorelli et al [19] demonstrated the differential expression of AAT at different lactation stages, comparing the expression of proteins extracted from mammary gland samples of two breeds (Sarda and Gentile di Puglia) dramatically differing in milk traits.…”

Section: Introductionmentioning

confidence: 99%

Splicing Variants of SERPINA1 Gene in Ovine Milk: Characterization of cDNA and Identification of Polymorphisms

Marchitelli¹,

Crisà²,

Mostarda³

et al. 2013

PLoS ONE

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The serine protease inhibitor, clade A, member 1 (SERPINA1) is the gene for a protein called alpha-1-antitrypsin (AAT), which is a member of the serine protease inhibitor (serpin) superfamily of proteins. By conformational change, serpins control several chemical reactions inhibiting the activity of proteases. AAT is the most abundant endogenous serpin in blood circulation and it is present in relatively high concentration in human milk as well as in bovine and porcine colostrum. Here we report for the first time the molecular characterization and sequence variability of the ovine SERPINA1 cDNA and gene. cDNAs from mammary gland and from milk were PCR amplified, and three different transcripts (1437, 1166 and 521bp) of the SERPINA1 gene were identified. We amplified and sequenced different regions of the gene (5’ UTR, from exon 2 to exon 5 and 3’ UTR), and we found that the exon-intron structure of the gene is similar to that of human and bovine. We detected a total of 97 SNPs in cDNAs and gene sequences from 10 sheep of three different breeds. In adult sheep tissues a SERPINA1 gene expression analysis indicated a differential expression of the three different transcripts. The finding reported in this paper will aid further studies on possible involvement of the SERPINA1 gene in different physiological states and its possible association with production traits.

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Differentially expressed mammary proteins during lactation in dairy sheep

Cited by 8 publications

References 38 publications

Proteomic Analysis of Isolated Plasma Membrane Fractions from the Mammary Gland in Lactating Cows

Proteomic Analysis of Isolated Plasma Membrane Fractions from the Mammary Gland in Lactating Cows

Use of Proteomics in the Study of Mastitis in Ewes

Splicing Variants of SERPINA1 Gene in Ovine Milk: Characterization of cDNA and Identification of Polymorphisms

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