Differentially expressed transcripts in stomach of Penaeus monodon in response to AHPND infection

Soonthornchai, Wipasiri; Chaiyapechara, Sage; Klinbunga, Sirawut; Thongda, Wilawan; Tangphatsornruang, Sithichoke; Yoocha, Thippawan; Jarayabhand, Padermsak; Jiravanichpaisal, Pikul

doi:10.1016/j.dci.2016.06.013

Cited by 53 publications

(38 citation statements)

References 75 publications

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“…monodon . Previous research has focussed on hepatopancreas, ovary, heart, muscle and eyestalk tissues 8 , 9 , in male and female gonads 10 , and in response to infection with Vibrio bacterial species capable of inducing acute hepatopancreatic necrosis disease 11 . In addition to such differential gene-expression studies, genomic data from next generation sequencing (NGS) methods has expanded greatly in recent years, particularly in the study of Pacific white shrimp ( Litopenaeus vannamei ) 3 , 6 , 12 – 23 .…”

Section: Introductionmentioning

confidence: 99%

De novo assembly, characterization, functional annotation and expression patterns of the black tiger shrimp (Penaeus monodon) transcriptome

Huerlimann

Wade

Gordon

et al. 2018

Sci Rep

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The black tiger shrimp (Penaeus monodon) remains the second most widely cultured shrimp species globally; however, issues with disease and domestication have seen production levels stagnate over the past two decades. To help identify innovative solutions needed to resolve bottlenecks hampering the culture of this species, it is important to generate genetic and genomic resources. Towards this aim, we have produced the most complete publicly available P. monodon transcriptome database to date based on nine adult tissues and eight early life-history stages (BUSCO - Complete: 98.2% [Duplicated: 51.3%], Fragmented: 0.8%, Missing: 1.0%). The assembly resulted in 236,388 contigs, which were then further segregated into 99,203 adult tissue specific and 58,678 early life-history stage specific clusters. While annotation rates were low (approximately 30%), as is typical for a non-model organisms, annotated transcript clusters were successfully mapped to several hundred functional KEGG pathways. Transcripts were clustered into groups within tissues and early life-history stages, providing initial evidence for their roles in specific tissue functions, or developmental transitions. We expect the transcriptome to provide an essential resource to investigate the molecular basis of commercially relevant-significant traits in P. monodon and other shrimp species.

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Section: Introductionmentioning

confidence: 99%

De novo assembly, characterization, functional annotation and expression patterns of the black tiger shrimp (Penaeus monodon) transcriptome

Huerlimann

Wade

Gordon

et al. 2018

Sci Rep

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“…Previous studies have shown that immune‐related genes such as penaeidins, crustin, and anti‐lipopolysaccharide factor were greatly altered during AHPND infection (Ge et al, ; Soonthornchai et al, ; Visetnan, Supungul, Tassanakajon, Donpudsa, & Rimphanitchayakit, ). Most of these genes are antimicrobial peptides produced by the Toll and immune deficiency pathways, which are the major innate immune pathways in shrimp (Li & Xiang, ).…”

Section: Discussionmentioning

confidence: 99%

The Rho signalling pathway mediates the pathogenicity of AHPND‐causing V. parahaemolyticus in shrimp

Lin

et al. 2018

Cellular Microbiology

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An emerging bacterial disease, acute hepatopancreatic necrosis disease (AHPND), is caused by strains of Vibrio parahaemolyticus with an additional AHPND-associated plasmid pVA1 encoding a virulent toxin (Pir ) that damages the shrimp's hepatopancreas. Like other species of Vibrio, these virulent strains initially colonise the shrimp's stomach, but it is not yet understood how the bacteria or toxins are subsequently able to cross the epithelial barrier and reach the hepatopancreas. Here, by using transcriptomics and system biology methods, we investigate AHPND-induced changes in the stomach of AHPND-causing V. parahaemolyticus (5HP)-infected shrimp and identify host molecular mechanisms that might explain how the integrity of the stomach barrier is compromised. We found that the expression of 376 unique genes was differentially regulated by AHPND infection. Gene ontology, protein interaction, and gene-to-gene correlation expression interaction analyses indicated that in addition to the immune system, a number of these genes were involved in cytoskeleton regulation by Rho GTPase. The involvement of Rho pathway regulation during AHPND pathogenesis was further supported by experiments showing that while Rho inhibitor pretreatment delayed the infection, pretreatment with Rho activator enhanced the pathogenicity of 5HP, and both the bacteria and toxin were detected sooner in the hepatopancreas. Further, disruption of the stomach epithelial structure was found in both Rho preactivated shrimp and in 5HP-infected shrimp. Taken together, we interpret our results to mean that Rho signalling helps to mediate AHPND pathogenesis in shrimp.

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“…Thus, we performed RNA-Seq and small RNA-Seq analyses of either NLHS-treated or NH control shrimp infected by VP AHPND to explore the genes, gene networks, and miRNAs that regulate these unknown immune mechanisms. www.nature.com/scientificreports www.nature.com/scientificreports/ Here, sequencing of mRNAs generated approximately 400 million (400 M) reads across 18 libraries for de novo assembly, which is 4-times and 6-times higher than those of recent transcriptome libraries from the hepatopancreas 18 and stomach 19 , respectively. These 18 libraries represent 3 biological replicates with an average of 22 M reads, each with 6 samples ensuring optimum statistical power to detect differentially expressed genes 20 .…”

Section: Discussionmentioning

confidence: 99%

MicroRNA and mRNA interactions coordinate the immune response in non-lethal heat stressed Litopenaeus vannamei against AHPND-causing Vibrio parahaemolyticus

Boonchuen

Maralit

Jaree

et al. 2020

Sci Rep

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While Vibrio parahaemolyticus (Vp AHpnD) has been identified as the cause of early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND) in shrimp, mechanisms of host response remain unknown. Understanding these processes is important to improve farming practices because this understanding will help to develop methods to enhance shrimp immunity. Pre-treatment of shrimp with 5-minute chronic non-lethal heat stress (NLHS) for 7 days was found to significantly increase Litopenaeus vannamei survival against Vp AHpnD infection. To elucidate the mechanism involved, mRNA and miRNA expression profiles from the hemocyte of L. vannamei challenged with Vp AHpnD after NLHS with corresponding control conditions were determined by RNA-Seq. A total of 2,664 mRNAs and 41 miRNAs were differentially expressed after the NLHS treatment and VP AHpnD challenge. A miRnA-mRNA regulatory network of differentially expressed miRNAs (DEMs) and differentially expressed genes (DEGs) was subsequently constructed and the interactions of DEMs in regulating the NLHSinduced immune-related pathways were identified. Transcriptomic data revealed that miRNA and mRNA interactions contribute to the modulation of NLHS-induced immune responses, such as the prophenoloxidase-activating system, hemocyte homeostasis, and antimicrobial peptide production, and these responses enhance Vp AHpnD resistance in L. vannamei. During its first outbreak, the devastating effect of early mortality syndrome (EMS) on the global shrimp industry was primarily caused by the lack of information regarding the disease and its causative agent 1. Later studies that were focused on mitigating this disease eventually identified the cause to be a toxin-harboring Vibrio parahaemolyticus, which causes Acute hepatopancreatic necrosis disease (AHPND) (VP AHPND) 2. This highlights how understanding disease etiology and a subsequent elucidation of host response can help to mitigate the effects of an outbreak. Understanding the functions of the shrimp immune system during disease progression is thus expected to create opportunities for the development of effective and efficient management strategies for VP AHPND infection. Likewise, information regarding the molecular mechanisms of AHPND tolerance can lead to platforms for the development of AHPND-resistant shrimp through selective breeding using markers mined from transcriptome analyses at different rearing conditions.

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Differentially expressed transcripts in stomach of Penaeus monodon in response to AHPND infection

Cited by 53 publications

References 75 publications

De novo assembly, characterization, functional annotation and expression patterns of the black tiger shrimp (Penaeus monodon) transcriptome

De novo assembly, characterization, functional annotation and expression patterns of the black tiger shrimp (Penaeus monodon) transcriptome

The Rho signalling pathway mediates the pathogenicity of AHPND‐causing V. parahaemolyticus in shrimp

MicroRNA and mRNA interactions coordinate the immune response in non-lethal heat stressed Litopenaeus vannamei against AHPND-causing Vibrio parahaemolyticus

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