1998
DOI: 10.1074/jbc.273.48.32071
|View full text |Cite
|
Sign up to set email alerts
|

Differentiating Keratinocytes Express a Novel Cytochrome P450 Enzyme, CYP2B19, Having Arachidonate Monooxygenase Activity

Abstract: The novel cytochrome P450, CYP2B19, is a specific cellular marker of late differentiation in skin keratinocytes. CYP2B19 was discovered in fetal mouse skin where its onset of expression coincides spatially (upper cell layer) and temporally (day 15.5) with the appearance of loricrin-expressing keratinocytes during the stratification stage of fetal epidermis. CYP2B19 is also present postnatally in the differentiated keratinocytes of the epidermis, sebaceous glands, and hair follicles. CYP2B19 mRNA is tightly cou… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

2
44
0

Year Published

1999
1999
2018
2018

Publication Types

Select...
7
2

Relationship

2
7

Authors

Journals

citations
Cited by 60 publications
(46 citation statements)
references
References 42 publications
2
44
0
Order By: Relevance
“…The HETEs can be subdivided into -terminal (-to -4-hydroxy), midchain, and bisallylic hydroxylations (Brash et al, 1995). Family 2 P450s metabolize arachidonate primarily to EETs (Daikh et al, 1994;Wu et al, 1996;Keeney et al, 1998;Luo et al, 1998). CYP2E1 differs in that its metabolism of arachidonic acid generates 90% -and (-1)-HETEs (Rifkind et al, 1995).…”
mentioning
confidence: 99%
“…The HETEs can be subdivided into -terminal (-to -4-hydroxy), midchain, and bisallylic hydroxylations (Brash et al, 1995). Family 2 P450s metabolize arachidonate primarily to EETs (Daikh et al, 1994;Wu et al, 1996;Keeney et al, 1998;Luo et al, 1998). CYP2E1 differs in that its metabolism of arachidonic acid generates 90% -and (-1)-HETEs (Rifkind et al, 1995).…”
mentioning
confidence: 99%
“…Regioisomeric EETs generated from endogenous arachidonic acid are present in mouse epidermal tissue and differentiating epidermal cell cultures, indicating the presence of catalytically active epoxygenases (Keeney et al, 1998;Du et al, 2005). Because existing evidence suggests mouse CYP2B19 is mainly responsible for cellular EET formation in mouse skin (Du et al, 2005), we set out to demonstrate that this keratinocyte-specific epoxygenase in epidermal microsomes is responsible for generating EETs from [1-…”
Section: Discussionmentioning
confidence: 99%
“…In the present study, we aimed to demonstrate that mouse epidermal microsomes generate EETs when reconstituted in the presence of [1- 14 C]arachidonate. To further establish roles for CYP2B19, we aimed to compare the regio-and stereospecificity of EETs formed by mouse epidermal microsomes with those generated by Escherichia coli-expressed CYP2B19 (Keeney et al, 1998). Instead of P450-derived EETs, we identified intermediates in a biosynthetic pathway not previously described in mouse skin that leads to formation of 8,.…”
mentioning
confidence: 99%
“…Although some AA-specific mono-oxygenases have been identified in epidermal keratinocytes [86,87], the overall contribution of CYP in cutaneous eicosanoid production remains to be explored. …”
Section: Cyp-derived Mediatorsmentioning
confidence: 99%