Optical remote sensing only provides information about the very thin surface layer of soil. Rainfall splash alters soil surface properties and its spectral reflectance. We analyzed the impact of rainfall on the success of soil organic matter (SOM) content (% by mass) detection and mapping based on optical remote sensing data. The subject of the study was the arable soils of a test field located in the Tula region (Russia), their spectral reflectance, and Sentinel-2 data. Our research demonstrated that rainfall negatively affects the accuracy of SOM predictions based on Sentinel-2 data. Depending on the average precipitation per day, the R2cv of models varied from 0.67 to 0.72, RMSEcv from 0.64 to 1.1% and RPIQ from 1.4 to 2.3. The incorporation of information on the soil surface state in the model resulted in an increase in accuracy of SOM content detection based on Sentinel-2 data: the R2cv of the models increased up to 0.78 to 0.84, the RMSEcv decreased to 0.61 to 0.71%, and the RPIQ increased to 2.1 to 2.4. Further studies are necessary to identify how the SOM content and composition of the soil surface change under the influence of rainfall for other soils, and to determine the relationships between rainfall-induced SOM changes and soil surface spectral reflectance.