Differentiation and Diagnosis of <i>Pseudocercosporella herpotrichoides</i> (Fron) Deighton with Genomic DNA Probes

Frei, Ursula; Wenzel, G.

doi:10.1111/j.1439-0434.1993.tb01421.x

Cited by 11 publications

(4 citation statements)

References 19 publications

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“…1991, 1993; Thomas et al. 1992; Frei and Wenzel 1993; Poupard et al. 1995; Takeuchi and Kuninaga 1996), and random amplified polymorphic DNAs [RAPDs, (Nicholson and Rezanoor 1994; Nicholson et al.…”

Section: Molecular Genetic Studies Of the Pathogensmentioning

confidence: 99%

“…During the 1990s, several genetic marker systems were used to study genetic diversity in eyespot pathogens. These were isozymes (Julian and Lucas 1990;Priestley et al 1992), restriction fragment length polymorphisms (RFLP) and other DNA-hybridizationbased marker systems (Nicholson et al 1991Thomas et al 1992;Frei and Wenzel 1993;Poupard et al 1995;Takeuchi and Kuninaga 1996), and random amplified polymorphic DNAs [RAPDs, (Nicholson and Rezanoor 1994;Papaikonomou and Lucas 1994;Vanova et al 2000)]. These studies mainly targeted identification of molecular fragments enabling discrimination of the two Oculimacula species.…”

Section: Molecular Genetic Studies Of the Pathogensmentioning

confidence: 99%

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Advances and Prospects in Wheat Eyespot Research: Contributions from Genetics and Molecular Tools

Wei

Muranty

Zhang

2011

Journal of Phytopathology

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Eyespot disease caused by the soil-borne facultative fungi Oculimacula yallundae and O. acuformis is the major component of the stem-base disease complex of wheat in temperate regions of the world with a cool and wet climate. In this review, we focus on results of genetic studies concerning both partners of the host-pathogen interaction. This comprises analyses of genetic diversity of the pathogen and identification of particular genes within it, evaluation and screening methods for host resistance, resistance sources and genetics of these resistances, breeding of resistant cultivars in wheat, and application of genetic markers in tagging and tracking of eyespot resistance genes. We also attempt to foresee some of the key issues and developments that may occur in future. The identification of markers tightly linked to eyespot resistance genes is the important research focus opening the door to marker-assisted selection of resistant varieties.

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Section: Molecular Genetic Studies Of the Pathogensmentioning

confidence: 99%

Section: Molecular Genetic Studies Of the Pathogensmentioning

confidence: 99%

Advances and Prospects in Wheat Eyespot Research: Contributions from Genetics and Molecular Tools

Wei

Muranty

Zhang

2011

Journal of Phytopathology

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“…Previously published techniques for the detection and discrimination of the two species include isozyme polymorphism (Julian and Lucas, 1990); Southern blot hybridization using specific DNA probes (Thomas et al., 1992; Frei and Wenzel, 1993); serological methods (Unger and Wolf, 1988; Lind, 1990; Priestly and Dewey, 1993); restriction fragment length polymorphism (RFLP; Nicholson et al., 1991) and polymerase chain reaction (PCR) amplification of the ribosomal internal transcribed spacer (ITS) region (Poupard et al., 1993; Gac et al., 1996). None of these techniques is quantitative; Southern blot hybridization may not be sufficiently sensitive to detect Oculimacula spp.…”

Section: Introductionmentioning

confidence: 99%

Using Real‐time PCR to Discriminate and Quantify the Closely Related Wheat Pathogens Oculimacula yallundae and Oculimacula acuformis

Walsh¹,

Korimbocus²,

Boonham³

et al. 2005

Journal of Phytopathology

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Oculimacula yallundae and O. acuformis are the causal agents of eyespot disease of wheat and other cereals. The two fungi react differently to the application of fungicides, but they cannot be distinguished visually and their occurrence is often masked by other less damaging pathogens. Current methods to detect and distinguish Oculimacula species are impractical when testing large numbers of samples. A real-time polymerase chain reaction (PCR) assay, suitable for largescale testing, was developed and used for quantitative detection and discrimination of O. yallundae and O. acuformis. As the available DNA sequences differ by only a small number of conserved nucleotide polymorphisms, three different methods were investigated to achieve the desired specificity. A combination of mutagenically separated PCR and a shortened primer gave the best specificity for O. yallundae and O. acuformis, respectively. Although the comparison illustrated the practicalities of each method, it was not possible to devise a fixed set of rules for the design of primers to discriminate two closely related sequences; it is assumed that sequence context is an overriding and difficult to predict factor, and that a range of empirical approaches may need to be taken to reach the specificity required.

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“…Molecular markers have been reported to give clear discrimination of W-and R-type isolates [Julian and Lucas, 1990;Nicholson et al, 1991;Priestley et al, 1992;Thomas et al, 1992;Frei and Wenzel, 1993;Nicholson et al, 1993;Nicholson and Rezanoor, 1994], but the techniques used were labour-intensive (analysis of isozyme polymorphism and detection of DNA restriction fragment length polymorphisms by Southern blot hybridization) or suffered from lack of reproducibility of the pattems (random amplification of polymorphic DNA (RAPD)). A rapid and reproducible method is the amplification of DNA sequences specific to each type of the fungus using the polymerase chain reaction (PCR).…”

Section: Introductionmentioning

confidence: 99%

Comparative study of morphological, cultural and molecular markers for the characterization ofPseudocercosporella herpotrichoides isolates

Gac¹,

Montfort²,

Cavelier³

et al. 1996

Eur J Plant Pathol

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Nirenberg's classification system and the polymerase chain reaction (PCR) combined with restriction enzyme digestion of an amplified ribosomal DNA fragment, were compared for the characterization of sixty isolates of Pseudocercosporella herpotrichoides, from various geographical areas and with differing fungicide sensitivity. With Nirenberg's system, it was possible to identify most isolates as P. herpotrichoides var. herpotrichoides or P. herpotrichoides var. acuformis. However, identification was slow and sometimes inconclusive as overlap occurred between the two varieties for all criteria examined. Molecular markers identified two distinct types among the isolates tested and generally good correlation was found between the PCR-based assay and Nirenberg's system, but the molecular assay was more accurate and faster.

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Differentiation and Diagnosis of Pseudocercosporella herpotrichoides (Fron) Deighton with Genomic DNA Probes

Cited by 11 publications

References 19 publications

Advances and Prospects in Wheat Eyespot Research: Contributions from Genetics and Molecular Tools

Advances and Prospects in Wheat Eyespot Research: Contributions from Genetics and Molecular Tools

Using Real‐time PCR to Discriminate and Quantify the Closely Related Wheat Pathogens Oculimacula yallundae and Oculimacula acuformis

Comparative study of morphological, cultural and molecular markers for the characterization ofPseudocercosporella herpotrichoides isolates

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