Differentiation And Identification Of Different Genotypes Of Sugar Beet (Beta Vulgaris L.) Using Dna Markers

Klyachenko, O.; Prysiazhniuk, Larysa

doi:10.31548/dopovidi2016.04.003

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…To obtain temperature-resistant sugar beet lines, we used dense primary callus from leaf explants of the original sugar beet genotypes obtained by us in previous studies. According to our genetic cluster analysis, the genotypes under study were divided into two groups (Kliachenko and Prysiazhniuk, 2014; Kliachenko and Prysiazhniuk, 2016). The fi rst group included variety Yaltushkivskyi Odnonasinnyi 64 and hybrids Yaltushkivskyi Odnonasinnyi 64, Ukrainskyi ChS 72, Ivanivskyi ChS 33 and Katiusha.…”

Section: Resultsmentioning

confidence: 99%

Obtaining Temperature-Resistant Sugar Beet Lines (<i>Beta vulgaris</i> L.)

Klyachenko¹,

Prysiazhniuk²,

Bokiy³

et al. 2023

Ecol. Eng. Environ. Technol.

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This study aimed to investigate the sugar beet genotypes for resistance to hyperthermia and obtain temperatureresistant lines. Nine hybrids and one variety of sugar beet were screened. Cotyledonary leaves and hypocotyls isolated from aseptic seedlings were used for induction of callus and subsequent subcultivation. To create hyperthermic conditions, the callus lines were maintained in thermostats at temperatures of +27 °C, +41 °C, +45 °C, and +47 °C. The effect of high temperatures on the callus tissue was assessed by the specific callus diameter index. The free proline was detected with chromatography. As result of callus tissue exposed to temperatures of +41 °C, 45 °C and 47 °C, on the 9th day of cultivation at high temperatures, significant differences were observed in the size and colouration of the callus tissues. At a moderate temperature (+41 °C), the growth of the callus mass was somewhat higher compared to the control. At a high temperature (+45 °C), the intensity of the growth processes decreased and ceased at a temperature of +47 °C. After transfer and subsequent cultivation of callus tissues in regeneration medium MSR -Murashige and Skoog medium for regeneration, all genotypes demonstrated the formation of morphological structures that initiated the formation of regenerated plants. The number of regenerated plants largely fluctuated over temperatures and almost was not related to genotypes. Consequently, the temperature-resistant lines obtained as a result of extreme heat treatment, differ in terms of the specific diameter of callus.

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Section: Resultsmentioning

confidence: 99%

Obtaining Temperature-Resistant Sugar Beet Lines (<i>Beta vulgaris</i> L.)

Klyachenko¹,

Prysiazhniuk²,

Bokiy³

et al. 2023

Ecol. Eng. Environ. Technol.

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“…DNA was isolated from 100 mg of green leaves of 5-day sprouts using CTAB and dissolved in TE buffer (Velikov, 2013;Roik et al, 2007). The authors used four RAPD markers (GEN 2-80-7: GCAGGTCGCG; GEN 1-80-5: ACCCCAGCCG; GEN 1-70-9/2: TGCAGCACCG; GEN 4-70-2: GGACCGACTG) (Smulders et al, 2010;Lučić et al, 2011;Klyachenko and Prysiazhniuk, 2016). The size of the alleles obtained by electrophoretic spectra was analysed in pairs of nucleotides using computer program TotalLab v.2.01.…”

Section: Dna Isolation and Pcrmentioning

confidence: 99%

Evaluation of Genetic Distances Correlations Among Sugar Beet Genotypes (Beta Vulgaris L.)

Klyachenko¹,

Prysiazhniuk²

2019

AgricultForest

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A search and a combination of different statistical methods for determining the similarity and difference between genotypes is a hot topic for breeding. In the formation of adaptive potential and appropriate protective mechanisms for the implementation of the adaptive potential of sugar beet, terpene compounds and saponins are involved. To determine polymorphism based on DNA of sugar beet materials under investigation, RAPD analysis was performed. A cluster analysis of the hybrids affinity was carried out in terms of the qualitative biochemical state of the system and DNA polymorphism. As a result, it was found that hybrids that demonstrated high activity of the secondary metabolism and, consequently, showed different ability to form a high adaptive potential in the conditions of long-term cultivation in vitro, formed separate clusters. With the aid of RAPD markers, the authors also determined two clusters that were formed on the basis of genotype affinity. In order to determine the correlations between genetic distances obtained by RAPD markers and terpene compounds, the Mantel test (linear correlation for Pearson) was performed. The values of coefficients r = 0.517 were obtained at α = 0.05. However, according to the data interpretation, the calculated value of p (0.088) was higher than the significance level of α = 0.05, which indicates the absence of correlations between the matrices under investigation. Thus, the revealed genotypic features of the secondary metabolism and genetic distances are additional characteristics for evaluation of the ecological plasticity of sugar beet plants, which is important in the breeding process.

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Complex selection in vitro of sugar beet (Beta vulgaris L.) for droughtproof and solismity

Klyachenko¹

2018

Biosystems

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Differentiation And Identification Of Different Genotypes Of Sugar Beet (Beta Vulgaris L.) Using Dna Markers

Cited by 3 publications

References 8 publications

Obtaining Temperature-Resistant Sugar Beet Lines (<i>Beta vulgaris</i> L.)

Obtaining Temperature-Resistant Sugar Beet Lines (<i>Beta vulgaris</i> L.)

Evaluation of Genetic Distances Correlations Among Sugar Beet Genotypes (Beta Vulgaris L.)

Complex selection in vitro of sugar beet (Beta vulgaris L.) for droughtproof and solismity

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