Differentiation between Candida species isolated from diabetic foot by fatty acid methyl ester analysis using gas chromatography

Missoni, Eduard; Rade, Desanka; Neđeral, Sandra; Kalenić, Smilja; Kern, Josipa; Babic, Verica Vazic

doi:10.1016/j.jchromb.2005.06.002

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…In this study, the mixed bacterial-fungal infection (14 %) was significantly higher than the fungal infection (4 %). The results of this study were compatible with the findings of Missoni et al [36] which reported a higher percentage of mixed bacterial-fungal infections (68 %) and twice the percentage of pure fungal infection (32 %). The conformation of C. dubliniensis from C. albicans was by using tobacco agar which correlated with the result of Khan et al [18].…”

Section: Discussionsupporting

confidence: 93%

“…So species identification of Candida was essential for successful management [37]. Moreover, candidal infection in diabetic foot was a rare entity and was previously described in five reports by Heald et al [34]; Missoni et al [36]; Yener et al [38]; Chellan et al [8] and Nithyalakshmi et al [9]. Since, our reports confirmed the prevalence of Candida albicans and non albicans in diabetic foot infections along with antifungal susceptibility pattern.…”

Section: Discussionsupporting

confidence: 81%

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Prevalence of yeast in diabetic foot infections

Sugandhi

Prasanth

2016

Int J Diabetes Dev Ctries

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Diabetic foot infections are a serious threat which cause a long term of hospitalization of diabetes patients and lead to amputations. Hence, prompt diagnosis is an important criterion in the treatment of these infections. This study was carried out to determine the prevalence of yeast in diabetic foot infections. The pus samples were collected from patients presenting with diabetic foot ulcer and processed for microbiological investigation. The patients investigated comprised of 82 males and 78 females. Most of the patients were grade I ulcers followed by grade II ulcers. Out of 160 samples, 138 samples were culture positive, in which yeast isolate Candida glabrata was the predominant isolate followed by Candida albicans, Candida dubliniensis, Candida krusei and Candida tropicalis. C. albicans and C. dubliniensis were differentiated by using tobacco agar due to the presence of hyphal fringes. The methylene blue sabouraud dextrose agar and corn meal agar were also used to differentiate C. albicans from non albicans. The antifungal susceptibility pattern showed 29 % of C. albicans was sensitive to Amphotericin B and Clotrimazole, while 75 % of C. glabrata was sensitive to Amphotericin B, Ketoconazole and Itraconazole. C. dublinensis and C. albicans showed the highest percentage of resistant to Ketoconazole, Fluconazole, Nystatin and Itraconazole. Thus, the results indicating that effective alternate drug of choice are required and the proper selection of antifungal agents will play an important role for the treatment of fungal foot infections. This study will also add substantial knowledge of yeast as one of the pathogenic organisms in diabetic foot infections.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 81%

Prevalence of yeast in diabetic foot infections

Sugandhi

Prasanth

2016

Int J Diabetes Dev Ctries

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“…PA possesses antimicrobial activity against numerous pathogens such as Streptococcus mutants, Streptococcus gordonii, Streptococcus sanguis, C. albicans, Aggregatibacter actinomycetemcomitans (Huang et al, 2011) but fails in Propionibacterium acnes (Yang et al, 2009). Palmitic acid is one of the major components in cellular fatty acids of Candida species such as C. parapsilosis, C. albicans, C. tropicalis, and C. famata (Missoni et al, 2005). Also, PA is a product of Fatty acid Synthase (FAS) complex and is crucial for subsequent desaturation of fatty acid in C. albicans (Nguyen et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Palmitic Acid Inhibits the Virulence Factors of Candida tropicalis: Biofilms, Cell Surface Hydrophobicity, Ergosterol Biosynthesis, and Enzymatic Activity

et al. 2020

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Biofilm is the fortitude of Candida species infections which eventually causes candidiasis in human. C. tropicalis is one of the predominant Candida species commonly found in systemic infections, next to C. albicans. In Candida species, biofilm maturity initiates irreversible surface attachment of cells and barricades the penetration of conventional antifungals. Hence, the current study investigated the antifungal and antivirulence potency of palmitic acid (PA) against C. tropicalis mature biofilm and its associated virulence factors. In vitro results revealed an effective inhibition of biofilm in PA-treated C. tropicalis, compared to C. albicans and C. glabrata. Also, PA reduced C. tropicalis mature biofilm at various time points. Further, PA treatment triggered apoptosis in C. tropicalis through ROS mediated mitochondrial dysfunction as demonstrated by confocal microscopic observation of PI, DAPI and DCFDA staining. PA regulated other virulence factors such as cell surface hydrophobicity, ergosterol biosynthesis, protease and lipase after 48 h of treatment. Downregulation of ERG11 (Lanosterol 14-alpha demethylase) was contributed to the reduction of ergosterol in PA-treated C. tropicalis. However, enhanced hyphal growth was observed in PA-treated C. tropicalis through upregulation HWP1 (Hyphal wall protein) and EFG1 (Enhanced filamentous growth). This study highlighted the antibiofilm and antivirulence potency of PA against C. tropicalis. Hence, PA could be applied synergistically with other antifungal agents to increase the efficacy for regulating NCAC infections.

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Current awareness on yeast

2006

Yeast

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (3 weeks journals ‐ search completed 7th. Dec. 2004)

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Differentiation between Candida species isolated from diabetic foot by fatty acid methyl ester analysis using gas chromatography

Cited by 6 publications

References 14 publications

Prevalence of yeast in diabetic foot infections

Prevalence of yeast in diabetic foot infections

Palmitic Acid Inhibits the Virulence Factors of Candida tropicalis: Biofilms, Cell Surface Hydrophobicity, Ergosterol Biosynthesis, and Enzymatic Activity

Current awareness on yeast

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