Differentiation-dependent expression of Adhfe1 in adipogenesis

Kim, Ji Young; Tillison, Kristin; Zhou, Shengli; Lee, Jun Ho; Smas, Cynthia M.

doi:10.1016/j.abb.2007.04.018

Cited by 28 publications

(20 citation statements)

References 89 publications

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“…5A, TNF␣ treatment results in a dramatic decrease in RIFL transcript in 3T3-L1 adipocytes to Ͻ10% of untreated time-matched cultures. As a control for the TNF␣ treatment, we assessed effects of TNF␣ on PPAR␥, SCD1, small adipocyte factor 1 (SMAF1), and CIDEC, transcripts established to be decreased markedly in TNF␣-treated adipocytes (22,24,25,55,57,79). As is apparent in the right four graphs in Fig.…”

Section: E337mentioning

confidence: 99%

Identification of RIFL, a novel adipocyte-enriched insulin target gene with a role in lipid metabolism

Ren

Kim

Smas

2012

American Journal of Physiology-Endocrinology and Metabolism

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392

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Ren G, Kim JY, Smas CM. Identification of RIFL, a novel adipocyte-enriched insulin target gene with a role in lipid metabolism. Am J Physiol Endocrinol Metab 303: E334 -E351, 2012. First published May 8, 2012; doi:10.1152/ajpendo.00084.2012.-To identify new genes that are important in fat metabolism, we utilized the Lexicon-Genentech knockout database of genes encoding transmembrane and secreted factors and whole murine genome transcriptional profiling data that we generated for 3T3-L1 in vitro adipogenesis. Cross-referencing null models evidencing metabolic phenotypes with genes induced in adipogenesis led to identification of a new gene, which we named RIFL (refeeding induced fat and liver). RIFL-null mice have serum triglyceride levels approximately one-third of wild type. RIFL transcript is induced Ͼ100-fold during 3T3-L1 adipogenesis and is also increased markedly during adipogenesis of murine and human primary preadipocytes. siRNA-mediated knockdown of RIFL during 3T3-L1 adipogenesis results in an ϳ35% decrease in adipocyte triglyceride content. Murine RIFL transcript is highly enriched in white and brown adipose tissue and liver. Fractionation of WAT reveals that RIFL transcript is exclusive to adipocytes with a lack of expression in stromal-vascular cells. Nutritional and hormonal studies are consistent with a prolipogenic function for RIFL. There is evidence of an approximately eightfold increase in RIFL transcript level in WAT in ob/ob mice compared with wild-type mice. RIFL transcript level in WAT and liver is increased ϳ80-and 12-fold, respectively, following refeeding of fasted mice. Treatment of 3T3-L1 adipocytes with insulin increases RIFL transcript Յ35-fold, whereas agents that stimulate lipolysis downregulate RIFL. Interestingly, the 198-amino acid RIFL protein is predicted to be secreted and shows ϳ30% overall conservation with the NH 2-terminal half of angiopoietin-like 3, a liver-secreted protein that impacts lipid metabolism. In summary, our data suggest that RIFL is an important new regulator of lipid metabolism.triglyceride; adipogenesis; differentiation WHITE ADIPOCYTES OF WHITE ADIPOSE TISSUE (WAT) are of major importance in lipid metabolism because they are involved in storage and release of triacylglycerol (10,30,31,42,58,81). Adipogenesis is the conversion of fibroblast-like preadipocytes to rounded lipid-filled mature adipocytes (5,35,47,48). Peroxisome proliferator-activated receptor-␥ (PPAR␥), a nuclear hormone receptor family member, functions as a master transcriptional regulator of adipogenesis (29,33,64,72). Multiple additional transcriptional and other signaling mechanisms positively or negatively regulate the process of adipogenesis (15,35,53,69). Because adipocytes are highly specialized for lipid metabolism, genes that are markedly upregulated during adipogenesis are strong candidates as new regulators of lipid metabolism. First established as an in vitro model of adipogenic conversion in the 1970s, the 3T3-L1 cell line has remained the dominant in vitro model of adipogene...

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Section: E337mentioning

confidence: 99%

Identification of RIFL, a novel adipocyte-enriched insulin target gene with a role in lipid metabolism

Ren

Kim

Smas

2012

American Journal of Physiology-Endocrinology and Metabolism

Self Cite

278

392

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“…ADHFE1 is an iron-sulfur enzyme and a member of the metaldependent alcohol dehydrogenase family and may have an important function in fatty acid metabolism (17,53). Its expression is likely linked to iron metabolism (16).…”

Section: Discussionmentioning

confidence: 99%

ADHFE1 is a breast cancer oncogene and induces metabolic reprogramming

Mishra

Tang

Putluri

et al. 2017

Journal of Clinical Investigation

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“…This enzyme has the highest affinity for ethanol and represents a metabolic barrier [first pass metabolism for dietary ethanols (27,72)]. There is also significant enrichment of message coding for the novel iron containing alcohol dehydrogenase (Adhfe1), which was recently cloned from human fetal brain (10) and has been found to play a role in adipocyte function (39). There is also slight gastric mucosal enrichment of messages coding for a variety of aldehyde dehydrogenases (Aldh1a1, 1a2, 3a1, and 6a1), confirming previous data (1).…”

Section: Expression Of Proteins Involved In Detoxification Of Food Comentioning

confidence: 99%

Selective gene expression by rat gastric corpus epithelium

Goebel

Stengel

Lambrecht

et al. 2011

Physiological Genomics

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The gastrointestinal (GI) tract is divided into several segments that have distinct functional properties, largely absorptive. The gastric corpus is the only segment thought of as largely secretory. Microarray hybridization of the gastric corpus mucosal epithelial cells was used to compare gene expression with other segments of the columnar GI tract followed by statistical data subtraction to identify genes selectively expressed by the rat gastric corpus mucosa. This provides a means of identifying less obvious specific functions of the corpus in addition to its secretionrelated genes. For example, important properties found by this GI tract comparative transcriptome reflect the energy demand of acid secretion, a role in lipid metabolism, the large variety of resident neuroendocrine cells, responses to damaging agents and transcription factors defining differentiation of its epithelium. In terms of overlap of gastric corpus genes with the rest of the GI tract, the distal small bowel appears to express many of the gastric corpus genes in contrast to proximal small and large bowel. This differential map of gene expression by the gastric corpus epithelium will allow a more detailed description of major properties of the gastric corpus and may lead to the discovery of gastric corpus cell differentiation genes and those mis-regulated in gastric carcinomas.transcriptome; stomach THE FUNCTION OF AN ORGAN DEPENDS on the genes and protein products expressed and their regulation. Much has been learnt by classical physiological or biochemical approaches, but the complexity of biology suggests that many functions may be overlooked by conventional approaches. If a complete and specific gene expression profile were available, a more realistic understanding of a specific organ's capabilities would ensue.The gastric corpus epithelium is the most complicated region of the gastrointestinal (GI) tract in terms of secretion, endocrine function, and differentiation due to its large variety of cell types. In this article, we present the specific transcriptome of the rat gastric corpus mucosa as obtained by whole rat genome microarray hybridization compared with three other mucosal segments of the columnar GI tract (proximal and distal small bowel, and colon). This is followed by statistical data subtraction to identify genes selectively expressed by the rat gastric corpus mucosa, but not by the other segments. Genes identified with this method most likely reflect functional importance in the stomach when considered in the context of relative rather than absolute expression levels. For example, many receptors are expressed at relatively low levels but their increased expression relative to other regions of the GI tract indicates significant function in the gastric secretory epithelium. The success of this method has been previously shown during transcriptomal analysis of purified suspensions of individual cell types, such as parietal and enterochromaffin-like (ECL) cells compared with the primary mucosal digest of the gastric corpus m...

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Differentiation-dependent expression of Adhfe1 in adipogenesis

Cited by 28 publications

References 89 publications

Identification of RIFL, a novel adipocyte-enriched insulin target gene with a role in lipid metabolism

Identification of RIFL, a novel adipocyte-enriched insulin target gene with a role in lipid metabolism

ADHFE1 is a breast cancer oncogene and induces metabolic reprogramming

Selective gene expression by rat gastric corpus epithelium

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