Differentiation of a cell line of mouse myeloid leukemia

Nagata, Kazuhiro; Takahashi, E.; Saito, Masashi; Ono, Junko; Kuboyama, Morio; Ogasa, Katsuhiro

doi:10.1016/0014-4827(76)90155-5

Cited by 32 publications

(3 citation statements)

References 12 publications

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“…In contrast, an increased synthesis in structural protein, such as the intermediate filament, vimentin, which is thought to be important for maintaining cell morphology, can be detected at 12 hr after incubation with IL-6 and reaches a peak at 48 hr after treatment (28). Similary, some lysosomal enzymes including acid phosphatase and cathepsis were induced after 48 hr of treatment with differentiation inducing factors (19).…”

Section: Discussionmentioning

confidence: 83%

“…Macrophagesproduces a numberof cytokines such as GM-CSFand IL-6 and lysosomal enzymes that are synthesized and trimmed in the ER (10,15,17,19). The ER is well-developed in differentiated macrophages and the ER resident proteins may be necessary for these differentiated functions.…”

Section: Discussionmentioning

confidence: 99%

“…During differentiation, Ml cells acquire phagocytic and locomotive activities, and undergo quantitative and qualitative changes in cytoskeletal proteins such as actin and vimentin (20,21,28). Differentiation is also accompanied by an increase in secretory proteins including many cytokines such as IL-6 (15,17) and GM-CSF (14), and lysosomal enzymes which undergo folding in the endoplasmic reticulum (ER) (10,19). There are manychaperones in the ER which are thought to recognize and stabilize partially folded intermediates of nascent polypeptide during their folding and assembly (6,7).…”

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confidence: 99%

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Expression and Phosphorylation of BiP/GRP78, a Molecular Chaperone in the Endoplasmic Reticulum, during the Differentiation of a Mouse Myeloblastic Cell Line.

Nakai

Kawatani

Ohi

et al. 1995

Cell Struct. Funct.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Expression and Phosphorylation of BiP/GRP78, a Molecular Chaperone in the Endoplasmic Reticulum, during the Differentiation of a Mouse Myeloblastic Cell Line.

Nakai

Kawatani

Ohi

et al. 1995

Cell Struct. Funct.

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In vitro differentiation of Rauscher‐virus‐induced myeloid leukemia cells

Ichikawa

Maeda

Horiuchi

1976

Intl Journal of Cancer

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Three cell lines were established in vitro from myeloid leukemias of C57Bl/6 strain mice which had been inoculated with Rauscher virus at birth. The use of heated conditioned medium from peritoneum proved helpful for the initial growth of leukemic cells. At early in vitro passage levels, cell line R433 differentiated only to macrophages after contact with conditioned medium from peritoneum but it appeared to have lost the ability to differentiate during an extended period of cultivation. Cell line R448 was cytologically promyelocyte. When exposed to conditioned medium, this cell line differentiated only to neutrophilic granulocytes. Cell line R453 maintained the ability to differentiate either to neutrophilic granylocytes or to macrophages. Single cells recloned three times still gave rise to both types of mature cells containing conditioned medium. Occasional cases of "spontaneous" differentiation in clonal lines of R453 also occurred.

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Differentiation of mouse myeloid leukemia cells is inhibited by a factor from non‐differentiating leukemia cells

Okabe

Hayashi

Honma

et al. 1978

Intl Journal of Cancer

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Mouse myeloid leukemia cells (MI) were induced to differentiate by a factor(s) (D-factor) in ascitic fluid. An inhibitory activity (I-activity) for the induction of differentiation was present in conditioned medium and lysate of MI cells resistant to the D-factor. The I-activity was non-dialyzable, heat-labile and protease-sensitive. Most of the activity was recovered in the fraction precipitated with 30-50% saturated ammonium sulfate. The fraction inhibited induction of phagocytic activity, migrating activity and morphological changes in MI cells, which are typical properties of differentiated MI cells. Low levels of I-activity were detected in conditioned medium or lysate of MI cells sensitive to the D-factor. The resistant MI cells were sensitized to the D-factor by treatment with a low concentration (5-10 ng/ml) of actinomycin D. The I-activity in conditioned medium of actinomycin D-treated resistant cells decreased with development of sensitivity to the D-factor. These results suggest that production of the I-activity in the resistant cells is closely associated with resistance of the MI cells to the D-factor.

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Differentiation of a cell line of mouse myeloid leukemia

Cited by 32 publications

References 12 publications

Expression and Phosphorylation of BiP/GRP78, a Molecular Chaperone in the Endoplasmic Reticulum, during the Differentiation of a Mouse Myeloblastic Cell Line.

Expression and Phosphorylation of BiP/GRP78, a Molecular Chaperone in the Endoplasmic Reticulum, during the Differentiation of a Mouse Myeloblastic Cell Line.

In vitro differentiation of Rauscher‐virus‐induced myeloid leukemia cells

Differentiation of mouse myeloid leukemia cells is inhibited by a factor from non‐differentiating leukemia cells

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