Differentiation of B and T mouse lymphocytes in cell suspension and smears

Padnos, Morton

doi:10.1038/259218a0

Cited by 10 publications

(4 citation statements)

References 9 publications

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“…has been made by both light and transmission microscopy [10,15,16]. Obviously, and given the importance of the subject, further work should be carried out.…”

Section: Resultsmentioning

confidence: 99%

Morphological Differences in Human Peripheral Blood Lymphocytes

Renau‐Piqueras¹

1978

Acta Haematol

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A morphological study using freeze-etching and scanning electron microscopy was carried out on lymphocytes from human peripheral blood. Freeze-etching electron microscopy of lymphocytes fractured at the level of the membrane shows the presence of two groups of cells according to their surface morphology: lymphocytes with a smooth surface and lymphocytes with a villous surface. Cells with surface features identical to the two types described by freeze-etching were also observed by scanning electron microscopy. Some lymphocytes with smooth surface showing uropods were also observed.

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“…has been made by both light and transmission microscopy [10,15,16]. Obviously, and given the importance of the subject, further work should be carried out.…”

Section: Resultsmentioning

confidence: 99%

Morphological Differences in Human Peripheral Blood Lymphocytes

Renau‐Piqueras¹

1978

Acta Haematol

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“…Some observers may see the shading as an elevation while others see it as a depression or one observer may see it either way at different times. None the less, the image intensity approximates the first derivative of the optical path difference of a transparent object or the geometric contour of an opaque object (Hoffman & Gross, 1975a, 1976.…”

Section: Performancementioning

confidence: 99%

“…When the slit is removed the modulation contrast objective can be used for bright field, dark field, polarization and fluorescence modes. The transmission modulation contrast system can be used in combination with reflected fluorescence techniques (Padnos, 1976).…”

Section: Componentsmentioning

confidence: 99%

The modulation contrast microscope: principles and performance

Hoffman

1977

Journal of Microscopy

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SUMMARY The modulation contrast microscope produces an image of high contrast and resolution. The image has a three‐dimensional appearance wherein a rounded object appears dark on one side, bright on the other with grey in between against a grey background. The performance features are optical sectioning, directionality, high resolution and control of contrast and coherence. A bright field microscope is converted to the modulation contrast microscope by adding the modulator, a special amplitude filter, in the objective. A slit aperture part of which is polarized is placed before the condenser. Below this is a rotatable polarizer. The modulator processes light from opposite gradients oppositely, that is brighter for one and darker for the other; thereby preserving the sign. The diffraction theory has been extended to show that gradient image intensity is the intensity of the zero order and when modified by the modulator creates a high contrast image. The modulation contrast microscope is simple and easy to adjust. It is useful in reflected and transmitted light systems, with plastic and glass vessels as well as in combination with fluorescence systems and polarization techniques. There is virtually no limit to the type of specimen that can be studied.

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“…Comparative studies by Padnos [1976] on the differentiation of B and T mouse lymphocytes have proved that the air-dried smear method is equivalent to the cell suspension technique.…”

Section: Methodsmentioning

confidence: 99%

Proportions of Mouse Erythrocyte Rosette-Forming Lymphocytes, Immunoglobulin-Bearing Cells and E Rosettes in Patients with Lymphoproliferative Diseases

Hunyadi¹,

Husz²,

Kenderessy³

et al. 1977

Acta Haematol

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The proportions of mouse erythrocyte rosette-forming (MERF) lymphocytes, immunoglobulin (Ig)-bearing cells and E rosettes were examined in the peripheral blood of patients with lymphoproliferative diseases. The proportion of MERF lymphocytes was considerably increased in chronic lymphocytic leukaemia. The correlation between the proportions of MERF and Ig-bearing cells, characteristic of healthy individuals, is not observed in the lymphoproliferative diseases. It is suggested that in a part of the patients with these diseases, the study of the proportion of MERF cells provides more accurate information on the proportion of lymphocytes of bursal origin than does counting of the Ig-bearing cells

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Differentiation of B and T mouse lymphocytes in cell suspension and smears

Cited by 10 publications

References 9 publications

Morphological Differences in Human Peripheral Blood Lymphocytes

Morphological Differences in Human Peripheral Blood Lymphocytes

The modulation contrast microscope: principles and performance

Proportions of Mouse Erythrocyte Rosette-Forming Lymphocytes, Immunoglobulin-Bearing Cells and E Rosettes in Patients with Lymphoproliferative Diseases

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