Differentiation of bacterial strains by means of surface enhanced FT-Raman spectroscopy

Abstract

The silver nanoparticle colloid was used to obtain surface enhanced Raman spectra of Listeria monocytogenes, Salmonela enterica, and Esherichia coli bacteria. The SERS spectra were captured using for excitation the near-infrared (1064 nm) laser radiation with reduced intensity, which ensured the prevention of the fluorescence background as well as photo-and thermal decomposition of the samples. It was found that the optimal size of silver nanoparticles for the enhancement of the Raman signal in the near-infrared spectral region is ca. 50 nm. The spectral data obtained in this study indicate that relative intensities of SERS spectral bands of bacteria can be used for spectral differentiation of bacteria. In case of Listeria, Salmonela, and Esherichia cells, the intensity ratio of spectral bands of adenine and cysteine can be used as a spectral marker for differentiation of the bacteria.

Elucidation of impact of heavy metal pollution on soil bacterial growth and extracellular polymeric substances flexibility

“…Unlike other techniques used in microbiology, the spectral examination is a relatively simple method for studying microbial surface molecular functional changes occurring during environmental stress conditions (Simonescu 2012 ). The bacterial vibrational spectra provide a wide range of biochemical properties about bacteria in a single spectrum, most importantly characteristics of the cell membrane (Kairyte et al 2012 ; Booyens and Thantsha 2014 ). Interestingly, the toxic metal-resistant single bacteria cell surface molecular IR and Raman spectra enlighten the membrane polarity and metal bio-absorbing macromolecular functional banding flexibility under ecological stress conditions (Lu et al 2011b ).…”