Differentiation of Biologically Distinct Cucumber Mosaic Virus Isolates by PAGE of Double-Stranded RNA

Pares, R. D.; Gillings, Michael R.; Gunn, L. V.

doi:10.1159/000150259

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…The electrophoresis pattern of purified dsRNA was indistinguishable from RNAs 1, 2 and 3 of the recent isolates, and the differences in relation to the CMV isolates used as controls, were real and reproducible, as reported by other authors (Pares et al., 1992). The satRNA was only detected in the recent CMV isolates after the second passage by mechanical inoculation through NX plants, under greenhouse conditions.…”

Section: Discussionsupporting

confidence: 85%

“…These CMV isolates could have been maintained as undetected haplotypes in mixed infection with prevalent haplotypes (Bonnet et al., 2005) or infecting until now alternative hosts as little focus of infection. This hypothesis seems to be opposite to the idea that a particular CMV type for a given host and geographical region appear to remain constant over time, as reported Pares et al. (1992) This could be specially true in this case, where a clear association of this particular CMV genotype with tomato plants has been demonstrated (Bonnet et al., 2005).…”

Section: Discussioncontrasting

confidence: 83%

“…3 Neighbour joining phylogenetic tree based on nucleotide sequences of a 382-bp fragment corresponding to the CP region of the RNA 3 from 15 CMV isolates belonging to IA, IB and II groups. Bootstrap values exceeding 80 are shown seems to be opposite to the idea that a particular CMV type for a given host and geographical region appear to remain constant over time, as reported Pares et al (1992) This could be specially true in this case, where a clear association of this particular CMV genotype with tomato plants has been demonstrated (Bonnet et al, 2005). However, the durability of CMV isolates over a long period of time, dependant on various factors, such as distinctive selection pressures by the aphid vector (Perry and Francki, 1992) or hosts competition, sometimes affected by the evolution of satRNAs.…”

Section: Discussionmentioning

confidence: 55%

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Reappearance of Cucumber mosaic virus Isolates Belonging to Subgroup IB in Tomato Plants in North‐eastern Spain

Aramburu¹,

Galipienso²,

López³

2007

Journal of Phytopathology

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A disease characterized by symptomless leaves and fruit decolouration, loss of consistency and mild deformation on ripening was detected in tomato fields in north-eastern Spain during 2003 and 2004. DAS-ELISA analysis revealed the presence of the Cucumber mosaic virus (CMV) in all diseased plants. CMV isolates were characterized by polyacrylamide gel electrophoresis (PAGE) analysis of double-stranded RNAs (dsRNAs) and nucleotide sequence analysis, and compared with some CMV isolates belonging to different subgroups used as controls. A total of 12 isolates obtained from the infected tomato plants selected for this study gave the same electrophoresis pattern for the three genomic dsRNAs, which was different to the patterns showed by the CMV isolates collected in the same region a few years ago. The identity of the complete nucleotide sequence of one of these CMV isolates and the partial sequence of other five isolates compared to the Tfn strain from Italy and the BAR92/1 isolate from tomato collected in Barcelona in 1992 was higher than 99%, both belonging to subgroup IB of CMV. The CMV isolates of this subgroup found in eastern Spain in previous studies were not detected after 1996. The nucleotide sequences of two isolates that were chosen as representatives of the CMV isolates more frequently detected in previous years revealed that they belonged to the CMV subgroups IA. The origin and the possible causes of reappearance of CMV IB isolates in north-eastern Spain are discussed.

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Section: Discussionsupporting

confidence: 85%

Section: Discussioncontrasting

confidence: 83%

Section: Discussionmentioning

confidence: 55%

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Reappearance of Cucumber mosaic virus Isolates Belonging to Subgroup IB in Tomato Plants in North‐eastern Spain

Aramburu¹,

Galipienso²,

López³

2007

Journal of Phytopathology

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“…The groups are named in different ways based on the procedures used: DTL and ToRS, U and N, I and II, WT and S, etc. [4,[7][8][9][10] Studies on CMV strain variation have been done by serology [11,12], dsRNA analysis [13], PCR-RFLP [14,15] and microarray [16]. According to phylogenetic analyses based on nucleotide (nt) sequences of various parts of the CMV genome, the virus strains and isolates form three subgroups known as IA, TB and II [5,17].…”

Section: Introductionmentioning

confidence: 99%

Detection, Differentiation and Phylogenetic Analysis of Cucumber Mosaic Virus Isolates from Cucurbits in the Northwest Region of Iran

2006

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One hundred and twenty three cucurbit samples with one or more symptoms of leaf mosaic, leaf distortion, fruit mosaic, stunting, mottling and yellowing were collected from several locations in the northwest region of Iran. Screening by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with a cucumber mosaic virus (CMV) polyclonal antibody, produced positive reactions from 13 samples. However, none of these positive samples reacted with a CMV subgroup-II (S-II)-specific monoclonal antibody in a triple antibody sandwich (TAS)-ELSIA. When total RNA from the CMV-infected samples was subjected to reverse transcription polymerase chain reaction (RT-PCR) with a pair of primers corresponding to the flanking regions of the virus coat protein (CP) gene, an expected DNA fragment of about 872 bp was amplified from 10 of the 13 isolates. This fragment covered the CP open reading frame (ORF) plus 92 and 123 bp of the 5' and 3' flanking regions, respectively. Restriction analysis with MspI (HpaII) was done on 9 of the PCR products and revealed a previously described CMV subgroup I (S-I) specific profile (537 and 335 bp fragments) for the isolates B13, B23, B5, SH5, SH17, S342 and S337, and an additional fragment, suggestive of combined profiles, was present for B13, SH5 and S342. Two other isolates, SH12 and B7 had a CMV S-II MspI profile (four visible fragments and a predicted non-visible 28-bp fragment on 2% agarose). Also, BsuRI (HaeIII) did not cut the PCR products characteristic of the CMV S-I specific MspI profile, whereas for the S-II isolates, BsuRI gave two fragments with sizes of approximately 559 and 313 bp. Nucleotide (nt) sequences of clones from the isolates B13, B23, SH5, SH17, S337 and SH12 were determined and aligned with those of previously published CMV strains and isolates. Consensus parsimonious trees constructed on the basis of the whole amplified region (841 nt excluding the primer sequences), CP ORF (nt or deduced amino acid data), or either of the flanking regions confirmed the RFLP data so that B13, B23, S337, SH5 and SH17 were placed in the CMV S-IA subclade, and SH12 in the S-II Clade. These analyses showed that both CMV S-I and S-II variants occur in the northwest region of Iran although S-I variants appeared to have a higher incidence.

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Isolation and Partial Characterisation of Double-Stranded RNA-Containing Viruses of Orchid Mycorrhizal Fungi

1998

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Differentiation of Biologically Distinct Cucumber Mosaic Virus Isolates by PAGE of Double-Stranded RNA

Cited by 7 publications

References 12 publications

Reappearance of Cucumber mosaic virus Isolates Belonging to Subgroup IB in Tomato Plants in North‐eastern Spain

Reappearance of Cucumber mosaic virus Isolates Belonging to Subgroup IB in Tomato Plants in North‐eastern Spain

Detection, Differentiation and Phylogenetic Analysis of Cucumber Mosaic Virus Isolates from Cucurbits in the Northwest Region of Iran

Isolation and Partial Characterisation of Double-Stranded RNA-Containing Viruses of Orchid Mycorrhizal Fungi

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