Reappearance of Cucumber mosaic virus Isolates Belonging to Subgroup IB in Tomato Plants in North‐eastern Spain

Abstract

A disease characterized by symptomless leaves and fruit decolouration, loss of consistency and mild deformation on ripening was detected in tomato fields in north-eastern Spain during 2003 and 2004. DAS-ELISA analysis revealed the presence of the Cucumber mosaic virus (CMV) in all diseased plants. CMV isolates were characterized by polyacrylamide gel electrophoresis (PAGE) analysis of double-stranded RNAs (dsRNAs) and nucleotide sequence analysis, and compared with some CMV isolates belonging to different subgroups used as controls. A total of 12 isolates obtained from the infected tomato plants selected for this study gave the same electrophoresis pattern for the three genomic dsRNAs, which was different to the patterns showed by the CMV isolates collected in the same region a few years ago. The identity of the complete nucleotide sequence of one of these CMV isolates and the partial sequence of other five isolates compared to the Tfn strain from Italy and the BAR92/1 isolate from tomato collected in Barcelona in 1992 was higher than 99%, both belonging to subgroup IB of CMV. The CMV isolates of this subgroup found in eastern Spain in previous studies were not detected after 1996. The nucleotide sequences of two isolates that were chosen as representatives of the CMV isolates more frequently detected in previous years revealed that they belonged to the CMV subgroups IA. The origin and the possible causes of reappearance of CMV IB isolates in north-eastern Spain are discussed.

Biological and Molecular Characterization of Two Distinct Tomato Strains of Cucumber mosaic virus Based on Complete RNA-3 Genome and Subgroup Specific Diagnosis

“…CMV has been reported to cause fruit necrosis in tomato in Hungary [22]. In Spain, subgroup-IB CMV causes symptomless leaves and discolouration of fruit [3]. In the present study, for the first time a subgroup-IB CMV causing grey patches on tomato fruit has been described and complete RNA-3 genome sequence has been characterized from India.…”

Resistance of Solanum species to Cucumber mosaic virus subgroup IA and its vector Myzus persicae

“…It is non-persistently transmitted by at least 80 aphid species, and has an extremely broad host range, infecting over 1,300 plant species in more than 500 genera of over 100 families including cereals, fruits, legumes, vegetables, ornamentals, weeds, etc. (Kaper and Waterworth 1981;Edwardson and Christie 1991;Palukaitis et al 1992;Aramburu et al 2007;García-Arenal and Palukaitis 2008).…”

Effect of cucumber mosaic virus infection on morphology, yield and phenolic contents of tomato

“…CMV was first reported in 1916 as the causal agent of plant diseases on cucumber plants as well as on Solanaceae, Fabaceae and Cucurbitaceae including cucumber (Cucumis sativus) and bottle gourd (Lagenaria siceraria) (Doolittle 1916). It is a type species of the genus Cucumovirus, in the family Bromoviridae, which is non-persistently transmitted by at least 75 aphid species and have an extremely broad host range, infecting more than 1000 plant species over 85 families of dicotyledons, monocotyledons, herbage and woody plants, horticultural crops and wild plants (Kaper and Waterworth 1981;Edwardson and Christie 1991;Palukaitis et al 1992;Aramburu et al 2007). CMV has been classified into two major subgroups, I and II, based on symptomatology, serology and sequence homology (Palukaitis et al 1992).…”