Differentiation of citrus bacterial canker strains in Korea by host range, rep-PCR fingerprinting and 16S rDNA analysis

Lee, Yong Hoon; Lee, Seungdon; Lee, Dong Hee; Ji, Sang Hye; Chang, Hyun Young; Heu, Sunggi; Hyun, Jae-Wook; Ra, Dong-Soo; Park, Eun Woo

doi:10.1007/s10658-007-9242-5

Cited by 11 publications

(9 citation statements)

References 11 publications

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“…Moreover, the analysis of 16S rDNA sequences did not give any discrimination between Saudi strains. Our results corroborate with the previous research that showed 16S rDNA sequences of different pathotypes of Xanthomonas causing citrus canker disease (A, B and C) were Xcc with a similarity of 99% (Lee et al, 2008). Fox et al (1992) reported that the 16S rDNA gene was considered unsuitable for discriminating and identifying closely related strains due to the high levels of sequence similarity in this region.…”

Section: Discussionsupporting

confidence: 91%

Distribution and pathotype identification of Xanthomonas citri subsp. citri recovered from south-western region of Saudi Arabia

Al-Saleh¹,

Widyawan²,

Saleh³

et al. 2014

Afr. J. Microbiol. Res.

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We investigated the distribution of Xanthomonas citri subsp. citri (Xcc) pathotypes in southwestern region of Saudi Arabia. A total of 76 Xanthomonas like strains were isolated from different citrus species showing bacterial canker symptoms from citrus commercial farms, backyard orchards and nurseries. These strains were subjected to biochemical, molecular and pathogenicity tests on leaves of grapefruit (Citrus paradisi). Based on symptoms induced on leaves of grapefruit, these strains were divided into two groups. There were 21 strains that induced typical erumpent canker lesions with watersoaked margin, belonging to Xcc type A. On the other hand, strains that produced flat necrotic lesions with water-soaked margin on grapefruit leaves belonged to Xcc A* type (55 strains). The physiological and biochemical tests, ImmuneStrip ® assays and 16S rDNA analysis confirmed the identity of the two Xcc pathotypes: A and A*. Our results indicate that, the two pathogenic variants co-exist in southwestern region of Saudi Arabia and this could further favour the generation of new genetic variants through recombination and horizontal genetic exchange. The generation of new aggressive pathotypes may threaten the citrus plantation in Saudi Arabia.

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Section: Discussionsupporting

confidence: 91%

Distribution and pathotype identification of Xanthomonas citri subsp. citri recovered from south-western region of Saudi Arabia

Al-Saleh¹,

Widyawan²,

Saleh³

et al. 2014

Afr. J. Microbiol. Res.

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“…Earlier studies considered primarily the usefulness of PFGE, AFLP, or rep-PCR markers in terms of their abilities to determine genetic relationships between Xanthomonas pathovars that are pathogenic to citrus and X. citri pv. citri pathological variants (16,19,39,44,68).…”

Section: Discussionmentioning

confidence: 99%

From Local Surveys to Global Surveillance: Three High-Throughput Genotyping Methods for Epidemiological Monitoring of Xanthomonas citri pv. citri Pathotypes

Ngoc

Vernière

Jarne

et al. 2009

Appl Environ Microbiol

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Asiatic citrus canker is a major disease worldwide, and its causal agent, Xanthomonas citri pv. citri, is listed as a quarantine organism in many countries. Analysis of the molecular epidemiology of this bacterium is hindered by a lack of molecular typing techniques suitable for surveillance and outbreak investigation. We report a comparative evaluation of three typing techniques, amplified fragment length polymorphism (AFLP) analysis, insertion sequence ligation-mediated PCR (IS-LM-PCR) typing, and multilocus variable-number tandem-repeat analysis (MLVA), with 234 strains originating from Asia, the likely center of origin of the pathogen, and reference strains of pathotypes A, A*, and A w , which differ in host range. The typing techniques were congruent in describing the diversity of this strain collection, suggesting that the evolution pattern of the bacterium may be clonal. Based on a hierarchical analysis of molecular variance, the AFLP method best described the genetic variation found among pathotypes whereas MLVA best described the variation found among individual strains from the same countries or groups of neighboring countries. IS-LM-PCR data suggested that the transposition of insertion sequences in the genome of X. citri pv. citri occurs rarely enough not to disturb the phylogenetic signal. This technique may be useful for the global surveillance of nonepidemiologically related strains. Although pathological characteristics of strains could be most often predicted from genotyping data, we report the occurrence in the Indian peninsula of strains genetically related to pathotype A* strains but with a host range similar to that of pathotype A, which makes the classification of this bacterium even more complicated.

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“…The rep-PCR dendrogram consisted of two main clusters with 51% genetic similarity among the rest of the isolates. In previous studies, this amount of genetic diversity was seen only amongst different species (formerly different strains) of CBC inducing bacteria (Cuberto and Graham 2002;Lee et al 2007). The other cluster was divided into five groups, containing the strains from two other closer states being surveyed.…”

Section: Resultsmentioning

confidence: 87%

“…Only the reproducible bands ranging from 0.2 to 3 kb were scored. Since combined analysis of rep-PCR primers showed a significant increase in differentiation capacity in other works (Trindade et al 2005;Lee et al 2007), combined data generated from two sets of primers were combined and then subjected to analysis. To estimate the genetic relationship among the strains, the similarity coefficient for pairs of isolates was calculated with Dice's coefficient index (Dice 1945).…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Xanthomonas Citri Subsp. Citri, Causal Agent of Citrus Canker

Arshadi¹,

Sijam²,

Awang³

2013

Journal of Plant Protection Research

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A total of 25 samples of canker disease from different part of West Malaysia were isolated from three different hosts. After various diagnostic tests, the samples were identified as Xanthomonas citri subsp. citri (Xcc), and were also pathogenic to four tested citrus species. Molecular characterization using rep-PCR fingerprinting was carried out on the isolates. Cluster analysis using the combined banding patterns of ERIC and BOX-PCR clearly divided the isolates into different clusters according to their geographical origin, but not to their host species. A relatively high amount of genetic diversity was observed among isolates, as a group of isolates from a more restricted part of Malaysia separated from the rest with relatively low similarity, indicating that there might be distinct pathotypes of the bacterium present in Malaysia.

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Differentiation of citrus bacterial canker strains in Korea by host range, rep-PCR fingerprinting and 16S rDNA analysis

Cited by 11 publications

References 11 publications

Distribution and pathotype identification of Xanthomonas citri subsp. citri recovered from south-western region of Saudi Arabia

Distribution and pathotype identification of Xanthomonas citri subsp. citri recovered from south-western region of Saudi Arabia

From Local Surveys to Global Surveillance: Three High-Throughput Genotyping Methods for Epidemiological Monitoring of Xanthomonas citri pv. citri Pathotypes

Genetic Diversity of Xanthomonas Citri Subsp. Citri, Causal Agent of Citrus Canker

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