1999
DOI: 10.1016/s0264-410x(99)00148-6
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Differentiation of convalescent animals from those vaccinated against foot-and-mouth disease by a peptide ELISA

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Cited by 95 publications
(60 citation statements)
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“…Ceditest FMDV-NS (CediDiagnostics B.V., Lelystad, The Netherlands) is a blocking ELISA based on baculovirus-expressed 3ABC antigen [24,25]. UBI FMD NS EIA (United Biochemical Inc., Hauppauge, NY, U.S.A.) is an indirect ELISA based on synthetic 3B peptide [23]. CHEKIT FMD-3ABC po (IDEXX Laboratories Inc., Westbrook, ME, U.S.A.) is an indirect ELISA based on E. coli-expressed 3ABC polypeptide [12].…”
Section: Serological Testing By Elisasmentioning
confidence: 99%
See 1 more Smart Citation
“…Ceditest FMDV-NS (CediDiagnostics B.V., Lelystad, The Netherlands) is a blocking ELISA based on baculovirus-expressed 3ABC antigen [24,25]. UBI FMD NS EIA (United Biochemical Inc., Hauppauge, NY, U.S.A.) is an indirect ELISA based on synthetic 3B peptide [23]. CHEKIT FMD-3ABC po (IDEXX Laboratories Inc., Westbrook, ME, U.S.A.) is an indirect ELISA based on E. coli-expressed 3ABC polypeptide [12].…”
Section: Serological Testing By Elisasmentioning
confidence: 99%
“…During the last decade, the use of immuno-enzymatic tests to detect anti-NSP antibodies and thus to assess virus circulation in susceptible populations has been studied extensively. Methods such as the latex beads agglutination test [26], enzyme-linked immunoelectrotransfer blot assay [2], enzyme-linked immunosorbent assay [2,3,5,8,9,10,12,16,17,19,[23][24][25] and multiplexed Luminex assay [9] have been employed for this purpose. These assays all need to be performed in the laboratory with different instrument and technical requirements, which restricts the use of these methods in the field and hence prolongs the time between diagnosis and response to this rapidly transmitted disease.…”
mentioning
confidence: 99%
“…It is reported that some peptides located in 2C, 2B, and 3B can be used to distinguish FMDV-infected from vaccinated animals, and enzymelinked immunosorbent assay (ELISA) methods based on these peptides were successfully developed. 5,9,13 Although these assays provide accurate and sensitive results, they require specialized equipment and technical expertise. For this reason, the authors have developed a simple and rapid assay (immunochromatographic test strip) based on these peptides for the detection of anti-FMDV NSP antibodies and compared the new assay with the available diagnostic peptide ELISAs.…”
mentioning
confidence: 99%
“…9 In addition, 1 group of workers have produced a recombinant form of 3AB1 associated with Glutathione S-transferase in a prokaryotic expression system, but the protein yield was very low in concentration. 10 The goal of this study was to produce a pure and highly concentrated preparation of recombinant 3AB1 for use in an FMDV-differential antibody enzyme-linked immunosorbent assay (ELISA).…”
mentioning
confidence: 99%