Differentiation of dental pulp stem cells into chondrocytes upon culture on porous chitosan-xanthan scaffolds in the presence of kartogenin

Westin, Cecília Buzatto; Trinca, Rafael Bergamo; Zuliani, C.C.; Coimbra, Ibsen Bellini; Moraes, Ângela Maria

doi:10.1016/j.msec.2017.07.005

Cited by 57 publications

(48 citation statements)

References 34 publications

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“…After a differentiation period of 6 weeks, aggrecan expression was reported in human DPSCs by Mata and colleagues [43]. Another factor might be the culture settings as many utilised scaffolds to improve the phenotype of DPSC-derived chondrocytes in vitro, including hydrogels containing poly(ethylene glycol) dimethacrylate (PEGDMA), methacrylated gelatin (GelMA) and hyaluronic acid (HA) [44] and chitosan-based scaffolds [45], but did not always test for aggrecan expression. In addition to differentiation time and culture environment, also hypoxic conditions and the addition of specific carbohydrates or growth factors might improve the expression of cartilage-specific components [24,46,47].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Monaco

Gervois

Beaumont

et al. 2020

Cells

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Osteoarthritis (OA) is a degenerative and inflammatory joint disorder with cartilage loss. Dental pulp stem cells (DPSCs) can undergo chondrogenic differentiation and secrete growth factors associated with tissue repair and immunomodulation. Leukocyte-and platelet-rich fibrin (L-PRF) emerges in regenerative medicine because of its growth factor content and fibrin matrix. This study evaluates the therapeutic application of DPSCs and L-PRF in OA via immunomodulation and cartilage regeneration. Chondrogenic differentiation of DPSCs, with or without L-PRF exudate (ex) and conditioned medium (CM), and of bone marrow-mesenchymal stem cells was compared. These cells showed differential chondrogenesis. L-PRF was unable to increase cartilage-associated components. Immature murine articular chondrocytes (iMACs) were cultured with L-PRF ex, L-PRF CM, or DPSC CM. L-PRF CM had pro-survival and proliferative effects on unstimulated and cytokine-stimulated iMACs. L-PRF CM stimulated the release of IL-6 and PGE2, and increased MMP-13, TIMP-1 and IL-6 mRNA levels in cytokine-stimulated iMACs. DPSC CM increased the survival and proliferation of unstimulated iMACs. In cytokine-stimulated iMACs, DPSC CM increased TIMP-1 gene expression, whereas it inhibited nitrite release in 3D culture. We showed promising effects of DPSCs in an in vitro OA model, as they undergo chondrogenesis in vitro, stimulate the survival of chondrocytes and have immunomodulatory effects.

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Section: Discussionmentioning

confidence: 99%

Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Monaco

Gervois

Beaumont

et al. 2020

Cells

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“…The most common methods used to evaluate the cartilage tissue engineering potential of DPSCs were histology and immunohistochemistry. Histology was used in seven out of the nine articles analyzed, 13,15,27,32,36,38,40 while immunohistochemistry was used in five studies. 13,27,32,37,39 Other methods included real-time polymerase chain reaction (RT-PCR; two studies), western blot (two studies), 13,38 and MTT (cell viability) assay (two studies).…”

Section: Evaluation Methodsmentioning

confidence: 99%

“…15,37 In two articles, only in vitro studies were performed. 39,40 Among the in vitro-only studies, one analyzed the culture and differentiation of DPSCs into chondrocytes using a specific scaffold, 40 and the other assessed the stem cell properties of DPSCs isolated by two different methods: enzymatic digestion of the pulp tissue or the explant method. 39…”

Section: In Vitro Designsmentioning

confidence: 99%

“…Seven of the studies reviewed in our study used different types of scaffolds, 13,15,27,[36][37][38]40 and each study used a different scaffold. The seven types of scaffolds consisted of collagen I/III, 15 alginate 3%, 36 beta-tricalcium phosphate (b-TCP), 37 poly-l-lactic acid/ polyethylene, 13 absorbable gelatin sponges (AGS), 38 chitosanxanthan gum matrices, 40 and fibrous polyglycolic acid (PGA). 27 Among these seven studies with scaffolds, one was an in vitro-only study that used a chitosan-xanthan scaffold to analyze the differentiation of DPSCs into chondrocytes.…”

Section: Scaffoldsmentioning

confidence: 99%

“…27 Among these seven studies with scaffolds, one was an in vitro-only study that used a chitosan-xanthan scaffold to analyze the differentiation of DPSCs into chondrocytes. 40 In the other two studies without the use of scaffolds, 32,39 one set of authors evaluated in vitro the stem cell properties of DPSCs obtained by two different methods of isolation, 39 and the other tested a treatment for systemic osteoarthritis using intravenously administered SHED. 32…”

Section: Scaffoldsmentioning

confidence: 99%

See 2 more Smart Citations

Systematic Review of Human Dental Pulp Stem Cells for Cartilage Regeneration

Fernandes

SantAnna

Frisene

et al. 2020

Tissue Engineering Part B: Reviews

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Background: Symptomatic cartilage lesions and early osteoarthritis produce significant clinical and economic burdens. Cartilage repair can improve the symptoms and delay arthroplasty. The complete healing of damaged cartilage with the consistent reproduction of normal hyaline cartilage has not yet been achieved. The choice of harvesting site might influence the cells' abilities to modulate immunologic and inflammatory responses. Recently, dental pulp has been shown to contain a stem cell niche consisting of dental pulp stem cells (DPSCs) that maintain their self-renewal capacity due to the active environment in the dental pulp of deciduous teeth. Objective: The aim of this study was to critically review the current literature on the potential and limitations of the use of dental pulp-derived mesenchymal stem cells in cell-based therapies for cartilage regeneration. Methods: An electronic, customized search of scientific articles was conducted using the PubMed/MEDLINE and EMBASE databases from their inception to December 2018. The inclusion criteria were applied, and the articles that described the use of DPSC in cartilage treatment were selected for complete evaluation. The articles were classified according to the scaffold used, experimental model, chondrogenic differentiation features, defect location, cartilage evaluation, and results. After the application of the eligibility criteria, a total of nine studies were selected and fully analyzed. Results: A variety of animal models were used, including mice, rats, rabbits, and miniature pigs, to evaluate the quality and safety of human DPSCs in the repair of cartilage defects. Among the articles, two studies focused on preclinical models of cartilage tissue engineering. Five studies implanted DPSCs in other animal sites. Conclusion: The use of DPSCs is a potential new stem cell therapy for articular cartilage repair. The preclinical evidence discussed in this article provides a solid foundation for future clinical trials.

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Progress in Articular Cartilage Tissue Engineering: A Review on Therapeutic Cells and Macromolecular Scaffolds

Zhao

Fan

Chen

et al. 2019

Macromolecular Bioscience

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Repair and regeneration of articular cartilage lesions have always been a major challenge in the medical field due to its peculiar structure (e.g., sparsely distributed chondrocytes, no blood supply, no nerves). Articular cartilage tissue engineering is considered as one promising strategy to achieve reconstruction of cartilage. With this perspective, the articular cartilage tissue engineering has been widely studied. Here, the recent progress of articular cartilage tissue engineering is reviewed. The ad hoc therapeutic cells and growth factors for cartilage regeneration are summarized and discussed. Various types of bio/macromolecular scaffolds together with their pros and cons are also reviewed and elaborated.

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Differentiation of dental pulp stem cells into chondrocytes upon culture on porous chitosan-xanthan scaffolds in the presence of kartogenin

Cited by 57 publications

References 34 publications

Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Systematic Review of Human Dental Pulp Stem Cells for Cartilage Regeneration

Progress in Articular Cartilage Tissue Engineering: A Review on Therapeutic Cells and Macromolecular Scaffolds

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