Differentiation of dietary regimene of Iberian swine by means of isotopic analysis of carbon and sulphur in hepatic tissue

González-Martı́n, I.; Pérez, Celia Rico; Méndez, J. Hernández; González, C Sánchez

doi:10.1016/s0309-1740(00)00126-1

Cited by 46 publications

(29 citation statements)

References 15 publications

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“…Mass spectrometry, on the other hand, has been successfully used to test the authenticity and the quality of several products, such as orange juice (Bricout & Koziet, 1987;Koziet et al, 1993), wine (Martin et al, 1988), honey (Brookes et al, 1991;White et al, 1998) and dairy products (Rossmann et al, 2000;Manca et al, 2001). Moreover, that technique has been used to characterize and differentiate the dietary regime of Iberian pigs, allowing their classification according to diet type during finishing (González-Martin et al, 1999) and for the certification of origin and quality of animal products (Hargin, 1996;Monin, 1998;González-Martin et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

The traceability of animal meals in layer diets as detected by stable carbon and nitrogen isotope analyses of eggs

Denadai¹,

Ducatti

Sartori

et al. 2008

Rev. Bras. Cienc. Avic.

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The aim of this study was to trace the inclusion of animal meals in layer diets by analyzing eggs and their fractions (yolk and albumen) using the technique of carbon and nitrogen isotopes. Two-hundred and eighty-eight (288) 73-week-old Shaver White layers, never fed animal ingredients, were randomly distributed in six treatments with six replicates each. The treatments were: control -corn and soybean meal based diet and five other experimental diets including bovine meat and bone meal (MBM); poultry offal meal (POM); feather meal (FM); feather meal and poultry offal meal (OFM), and poultry offal meal, feather meal, and meat and bone meal (MBOFM). The isotopic results were submitted to multivariate analysis of variance. Ellipses were determined through an error matrix (95% confidence) to identify differences between treatments and the control group. In the albumen and yolk of all experimental treatments were significantly different from the control diet (p < 0.05). In summary, the stable isotope technique is able to trace the animal meals included in layer feeds in the final product under these experimental conditions.

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Section: Introductionmentioning

confidence: 99%

The traceability of animal meals in layer diets as detected by stable carbon and nitrogen isotope analyses of eggs

Denadai¹,

Ducatti

Sartori

et al. 2008

Rev. Bras. Cienc. Avic.

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“…Frecuentemente, los recursos disponibles son insuficientes para cubrir la demanda, dando en ocasiones lugar a la alimen tación artificial de algunos de los cerdos. El análisis de las re laciones isotópicas 13 C/ 12 C de los ácidos grasos Palmítico, Es teárico, Oleico y Linoleico mediante GCCIRMS permite diferenciar entre animales criados al estilo tradicional (acaba dos en montanera) y aquellos alimentados con piensos. El va lor de δ 13 C C18:1 = -25.9‰ es un buen indicador, pero la correc ta asignación de una muestra dada requiere el empleo de los cuatro ácidos grasos indicados y su tratamiento estadístico mediante análisis de componentes principales.…”

Section: Resumen Análisis De áCidos Grasos Mediante Gc-c-irms Como Heunclassified

“…C isotope ratios essentially reflect the diet of an organism (although enriched by about 1‰; DeNiro and Epstein, 1978), and its application to the characterization of whole Iberian swine products has been reported before (GonzálezMartín, et al, 2001;GonzálezMartín, et al,1999). The use of formulated feeds with added fats, selected to result in a given fatty acid profile, however, can show up in (at least some of) the results, rendering them at best nonconclusive.…”

Section: Gc-c-irms Analysis Of Fames As a Tool To Ascertain The Diet mentioning

confidence: 99%

“…Compound-Specific Isotopic Characterization techniques (CSIC), however, are more difficult to falsify, since multiple parameters are determined simultaneously on a single sample. GCCIRMS methods (Gas Chromatography Combustion Isotope Ratio Mass Spectrometry) allow for the determination of 13 C/ 12 C ratios in several FAMEs, leading to a "fingerprint" that is characteristic of each individual sample (see Meier Augenstein, 2002, for a review). In this regard, the techniques applied make use of formerly official methods, in the sense that they analyze the chromatographic profile of the swine fat, but go a step beyond by determining its C isotopic signature as well.…”

Section: Gc-c-irms Analysis Of Fames As a Tool To Ascertain The Diet mentioning

confidence: 99%

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GC-C-IRMS analysis of FAMEs as a tool to ascertain the diet of Iberian pigs used for the production of pork products with high added value

Récio¹,

Martín²,

Raposo³

2013

Grasas y Aceites

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Taste, quality and perceived health benefits result in a high demand for both green and mature pork products derived from the Iberian bred swine. These characteristics are directly related to diet and breeding style (free ranging in a particular Mediterranean ecosystem: the Dehesa). Given that current demand is not matched by the available resources, animal feed is increasingly used. GC-C-IRMS analysis of Palmitic, Stearic, Oleic and Linoleic Acid methyl esters allows for differentiating between pork raised in the traditional way and that fattened on animal feed. Although a value of δ¹³C_C18:1 = –25.9‰ is a good discriminator, exploratory analysis of principal canonical components is required to properly assign any unknown sample.

El sabor, la calidad y los efectos saludables de los derivados del cerdo ibérico dan lugar a una elevada demanda de los mismos, tanto en fresco como curados. Las características mencionadas responden a una dieta y crianza específicas (libres en la Dehesa, alimentándose de lo que ésta produce). Frecuentemente, los recursos disponibles son insuficientes para cubrir la demanda, dando en ocasiones lugar a la alimentación artificial de algunos de los cerdos. El análisis de las relaciones isotópicas ¹³C/¹²C de los ácidos grasos Palmítico, Esteárico, Oleico y Linoleico mediante GC-C-IRMS permite diferenciar entre animales criados al estilo tradicional (acabados en montanera) y aquellos alimentados con piensos. El valor de δ¹³C_C18:1 = –25.9‰ es un buen indicador, pero la correcta asignación de una muestra dada requiere el empleo de los cuatro ácidos grasos indicados y su tratamiento estadístico mediante análisis de componentes principales

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“…Porém, a espectrometria de massas, por meio da análise da razão isotópica do carbono ( 13 C/ 12 C), tem sido usada com sucesso para testar a autenticidade, a qualidade e a origem geográfica de vários produtos, tais como: sucos de frutas (Bricout & Koziet, 1987;Koziet et al, 1993), vinhos (Martin et al, 1988), méis (Brookes et al, 1991;White et al, 1998) e produtos lácteos (Rossmann et al, 2000;Manca et al, 2001). A razão isotópica do carbono, em associação à do nitrogênio ( 15 N/ 14 N), permitiu a certificação da origem geográfica e tipos de alimentação de ovinos (Piasentier et al, 2003), a caracterização e diferenciação do regime dietético de suínos ibéricos (González-Martin et al, 1999) e a rastreabilidade de produtos de origem animal na dieta de frangos de corte (Oliveira, 2005;Carrijo et al, 2006;Gottmann et al, 2008) e de codornas (Móri et al, 2007), no produto final.…”

Section: Introductionunclassified

Rastreabilidade da farinha de carne e ossos bovinos em ovos de poedeiras alimentadas com ingredientes alternativos

Denadai

Ducatti²,

Sartori

et al. 2009

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi detectar traços de farinha de carne e ossos bovinos, em ovos de poedeiras alimentadas com dietas comerciais com inclusão de ingredientes vegetais alternativos e leveduras. A detecção foi feita pela técnica dos isótopos estáveis do carbono e do nitrogênio. Foram utilizadas 384 poedeiras, distribuídas aleatoriamente em oito tratamentos. Os tratamentos consistiram de uma dieta-controle -à base de milho e farelo de soja -e sete dietas com inclusão de farinha de carne e ossos bovinos, acrescidas ou não de outros ingredientes (farelo de trigo, quirera de arroz, farelo de algodão, glúten de milho, levedura de cana e levedura de cerveja). No 35 o dia, foram tomados aleatoriamente 24 ovos por tratamento: 12 para análise de ovo e 12 para análise de gema e albúmen, em separado. Após análise isotópica de carbono e nitrogênio, os resultados foram submetidos à análise multivariada de variância. As médias dos pares isotópicos dos ovos, gema e albúmen, em todos os tratamentos, diferiram daquelas do tratamento-controle. A técnica dos isótopos estáveis permite detectar, nos ovos, gema e albúmen, a farinha de carne e ossos bovinos utilizada na dieta de poedeiras, mesmo com a inclusão de outros ingredientes vegetais e leveduras.Termos para indexação: Gallus gallus, carbono-13, certificação, encefalopatia espongiforme bovina, isótopos estáveis, nitrogênio-15. Traceability of bovine meat and bone meal in eggs from laying hens fed with alternative ingredientsAbstract -The objective of this work was to detect traces of the bovine meat and bones meal in eggs of laying hens fed on commercial diets including alternative plant ingredients and yeast. The bovine meal detection was performed through carbon and nitrogen stable isotope technique. Three hundred eighty-four laying hens were randomly distributed in eight treatments. Treatments consisted of one control diet -a corn-and-soybean based meal -and seven diets with bovine meat and bone meal, including or not other ingredients (wheat bran, rice, cottonseed meal, corn gluten meal, sugarcane yeast and brewer yeast). At the 35 th day, 24 eggs per treatment were randomly collected, 12 for egg analyses and 12 for yolk and albumen analyses. After isotopic analysis of carbon and nitrogen, the results were submitted to multivariate analysis of variance. The average isotopic pairs of egg, yolk and albumen of all treatments differed from those of the control. The stable isotope technique allowed detection of bovine meat and bone meal in eggs, yolk and albumen, produced by laying hens fed on diets with inclusion of plant ingredients and yeast.

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Differentiation of dietary regimene of Iberian swine by means of isotopic analysis of carbon and sulphur in hepatic tissue

Cited by 46 publications

References 15 publications

The traceability of animal meals in layer diets as detected by stable carbon and nitrogen isotope analyses of eggs

The traceability of animal meals in layer diets as detected by stable carbon and nitrogen isotope analyses of eggs

GC-C-IRMS analysis of FAMEs as a tool to ascertain the diet of Iberian pigs used for the production of pork products with high added value

Rastreabilidade da farinha de carne e ossos bovinos em ovos de poedeiras alimentadas com ingredientes alternativos

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