The traceability of animal meals in layer diets as detected by stable carbon and nitrogen isotope analyses of eggs

Denadai, Juliana Célia; Ducatti, Carlos; Sartori, José Roberto; Pezzato, Antônio Celso; Móri, Cleusa; Gottmann, Rosana; Mituo, Mariela Akie Okino; Bordinhon, André Moreira

doi:10.1590/s1516-635x2008000300010

Cited by 11 publications

(15 citation statements)

References 24 publications

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“…Both d 13 C and d 15 N isotope enrichment of the breast muscle was detected in birds that were fed the diets containing POM sometime during the rearing period. This 13 C and 15 N enrichment when poultry were fed animal by-product meals was also observed by Oliveira (2010), Carrijo et al (2006), Móri et al (2007Móri et al ( , 2008, Denadai et al (2008) and Gottmann et al (2008).…”

Section: Resultssupporting

confidence: 81%

“…Carbon isotope ratios ( 13 C/ 12 C) combined with nitrogen isotope ratios ( 15 N/ 14 N) in the final product allow tracing the inclusion of animal by-product meals in broiler (Carrijo et al, 2006;Gottmann et al, 2008;Oliveira, 2010), layer (Denadai et al, 2008), and quail (Móri et al, 2007) feeds. However, this technique had not been employed yet in studies with poultry reared for long periods, where theoretically animal by-products could be included and withdrawn from the feeds without being detected by this method.…”

Section: The Use Of Carbon and Nitrogen Stable Isotopes For The Detecmentioning

confidence: 99%

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The use of carbon and nitrogen stable isotopes for the detection of poultry offal meal in meat-type quail feeds

Sernagiotto¹,

Ducatti

Sartori

et al. 2013

Rev. Bras. Cienc. Avic.

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The objective of the present study was to trace the inclusion of poultry offal meal (POM) in the diet of meat-type quails reared for a long period using the technique of stable isotopes. A number of 320 quails were randomly distributed into eight treatments: vegetable diet (T1), and a diet containing 8% POM were fed until the end of the experimental period (T2) or replaced by the vegetable diet on day 42 (T3), 56 (T4), 70 (T5), 84 (T6), 98 (T7), and 112 (T8). Breast muscle samples were collected from four birds randomly selected per treatment every 14 days. The obtained isotope results were submitted to multivariate analysis of variance (MANOVA) with the aid of the GLM procedure of statistical SAS program. Treatments were different from T1 when birds were sacrificed at least two weeks after the diet was changed. T2 results were different from T1 in all evaluated periods. It was concluded that it is possible to trace poultry offal meal inclusion in a strictly vegetable diet after the diet was changed for at least 14 days.

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Section: Resultssupporting

confidence: 81%

Section: The Use Of Carbon and Nitrogen Stable Isotopes For The Detecmentioning

confidence: 99%

The use of carbon and nitrogen stable isotopes for the detection of poultry offal meal in meat-type quail feeds

Sernagiotto¹,

Ducatti

Sartori

et al. 2013

Rev. Bras. Cienc. Avic.

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“…In order to carry out the isotopic analysis on the experimental diets and feces, the samples were thawed out, dried in a forced draft dryer at 56 1C over a period of 48 h and cryogenically ground (cryogenic grinder model Spex-6750 freezer/mill, Metuchen, EUA), to À196 1C, according to the methodology described by Carrijo et al (2006), Denadai et al (2008) and Mó ri et al (2007). During grinding, approximately 2.0 g of the sample was collected in a poly-carbon flask together with a magnetic bar, which was duly closed and immersed in liquid nitrogen.…”

Section: Methodsmentioning

confidence: 99%

Stable isotopes for determining carbon turnover in sheep feces and blood

et al. 2012

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a b s t r a c tThe objective of this work was to evaluate carbon turnover and half-life in feces and blood from sheep fed on C 3 and C 4 plant diets, using the stable isotope technique. Eight Santa Ines sheep were randomly distributed between two treatments: the first consisted of animals that were fed alfalfa hay, and the second consisted of animals that were fed corn silage only. Function of time was used to measure carbon turnover rate. At steady state, the half life for carbon isotopic enrichment between food and feces is 1.2 and 1.0 days for alfalfa hay and corn silage treatments, respectively. For blood data collection the time was insufficient to reach the isotope equilibrium level, indicating a slow carbon exchange between diet and blood. It is concluded that blood has a slow turnover, indicating the isotope signal for the former diets. Feces, by contrast, have a quick turnover, indicative of their recent diets.

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“…The difference of δ 13 C values between these plants is large enough so that the relative amounts of C 3 and C 4 plants eaten by an animal can be determined from the δ 13 C value of its carbon (DeNiro and Epstein, 1978), enabling analysis of turnover of stable isotopes in animal tissues (Tieszen et al, 1983;Hobson andClarck, 1992, 1993;Hobson, 1995;Hobson and Bairlein, 2003;Ayliffe et al, 2004;Buchheister and Latour, 2010;Oppel and Powell, 2010). More recently, this technique has been used in traceability studies of AOI in poultry feed via tissue analysis (Carrijo et al, 2006;Oliveira et al, 2010;Denadai et al, 2008;Sernagiotto et al, 2013) or identify the origin of a food (Rock, 2012). Furthermore, this technique has been used to evaluate the effect of natural additives, alternative to APE, on the structure of the intestinal mucosa.…”

Section: Introductionmentioning

confidence: 99%

Estimation of the metabolic rate by assessing carbon-13 turnover in broiler tissues using the stable isotope technique

et al. 2018

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A B S T R A C TThe aim of this study was to estimate the metabolic rate of broiler tissues by assessing carbon turnover in different growth phases by using the stable isotope technique and by evaluating the natural changes in Carbon-13 ( 13 C) between C3 and C4 cycle plants. Six-hundred 1-day-old broiler chicks were divided into six groups and subjected to dietary changes containing different isotopic values at 7, 14, 21, 28, and 35 days of age. Birds of Group 1 received diets composed predominantly of C 3 cycle plants shortly after accommodation, while birds of other groups remained predominantly fed with C 4 diet. The diet of Group 2 was mainly replaced by C 3 diet on the 7th day of age. Group 3 started to receive C 3 diet on the 14th day of age, whereas the diet of Group 4 was replaced on the 21st, Group 5 on the 28th. In Group 6, the substitution occurred on the 35th day of age. Samples of the pectoral muscle, keel, tibia, legs muscle, intestinal mucosa, blood, plasma, and feathers were collected. Carbon isotope ratios were determined by mass-spectrometry of isotope ratios. To measure the speed of carbon substitution after a set interval (turnover), a time exponential function was employed and obtained by exponential equations of the first order calculated with Minitab ® 16 software. The carbon half-life varied from 1.78 to 8.20 days for the pectoral muscle, from 1.91 to 12.24 days for the keel, from 2.32 to 10.71 days for the tibia, from 1.87 to 9.43 days for the leg muscle, from 0.8 to 1.58 days for the intestinal mucosa, from 0.64 to 1.71 days for the plasma, from 2.61 to 11.07 days for blood, and from 1.84 to 28.41 days for the feathers during the total growth phase. With the exception of tibia and blood, all other tissues presented a high metabolic rate in the first week of the broilers' life. The metabolic rate of tissues is faster in younger animals, and the metabolism slows down as the broilers get older. The metabolic rates in the pectoral muscle, keel, tibia, and feathers decreased with age, whereas those of the plasma and intestinal mucosa were high in all evaluated periods; thus, these tissues can be used to study traceability of feed at all stages of the broilers' life.

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The traceability of animal meals in layer diets as detected by stable carbon and nitrogen isotope analyses of eggs

Cited by 11 publications

References 24 publications

The use of carbon and nitrogen stable isotopes for the detection of poultry offal meal in meat-type quail feeds

The use of carbon and nitrogen stable isotopes for the detection of poultry offal meal in meat-type quail feeds

Stable isotopes for determining carbon turnover in sheep feces and blood

Estimation of the metabolic rate by assessing carbon-13 turnover in broiler tissues using the stable isotope technique

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