Differentiation of feline coronavirus type I and II infections by virus neutralization test

Shiba, N; Maeda, Ken; Kato, Hirotomo; Mochizuki, Masahito; Iwata, Hiroyuki

doi:10.1016/j.vetmic.2007.04.031

Cited by 50 publications

(52 citation statements)

References 21 publications

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“…The prevalence of type I and II FCoVs has been surveyed in many countries using serological and molecular assays (Addie et al, 2003;Benetka et al, 2004;Hohdatsu et al, 1992;Kummrow et al, 2005;Shiba et al, 2007;Vennema, 1999). In concordance with others findings, our results indicate that the majority of FCoV infection in Taiwan is due to type I viruses (88.7%).…”

Section: Discussionsupporting

confidence: 90%

“…Type II virus is more likely to cause acute infections that result in either FIP or recovery without persistence. This distinction might explain why, in the epidemiological studies from various countries, the prevalence of type II virus is consistently lower than that of type I virus (Addie et al, 2003;Benetka et al, 2004;Hohdatsu et al, 1992;Kummrow et al, 2005;Shiba et al, 2007;Vennema, 1999).…”

Section: Discussionmentioning

confidence: 97%

“…USA (Vennema, 1999), UK (Addie et al, 2003), Japan (Hohdatsu et al, 1992;Shiba et al, 2007), Austria (Benetka et al, 2004), and Switzerland (Kummrow et al, 2005). Regardless of which assays were used, the majority of infections were attributed to type I FCoV.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity and correlation with feline infectious peritonitis of feline coronavirus type I and II: A 5-year study in Taiwan

Lin

Wang

et al. 2009

Veterinary Microbiology

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The outcomes of feline coronavirus (FCoV) infection vary greatly from asymptomatic or mild enteric infection to fatal feline infectious peritonitis (FIP). On the basis of in vitro neutralization tests, FCoVs can be divided into two serotypes. To explore the correlation between different types of FCoV and FIP, clinical specimens collected from 363 naturally infected cats during 2003-2007 were analyzed. Amplification of a portion of the S gene from the FCoV was performed and a total of 222 cases were differentiated. Among them, 197 (88.7%) cats were type I-positive, 13 (5.9%) were type II-positive, and 12 (5.4%) were positive for both types. Irrespective of the predominance of type I FCoV infection in Taiwan, type II FCoV demonstrated a significantly higher correlation with FIP (p<0.01). Analysis of partial S gene sequences of the local type I and II FCoVs strains revealed that type I viruses were more genetically divergent (6.2-11.7%) than type II viruses (0.6-3.2%) within the 5-year study period. The higher genetic diversity of type I FCoVs might be due to the larger infected cat population and to the long period of viral persistence in asymptomatic cats in comparison to type II viruses.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 97%

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Genetic diversity and correlation with feline infectious peritonitis of feline coronavirus type I and II: A 5-year study in Taiwan

Lin

Wang

et al. 2009

Veterinary Microbiology

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“…FIPV is serologically classified into types I and II, but both types induce FIP in cats. These 2 types do not serologically cross-react with each other (Pedersen et al, 1984;Shiba et al, 2007). Since type II FIPV more actively replicates in cell lines and is more pathogenic for cats, previous studies on FIPV and anti-FIPV agents were performed using type II FIPV; however, studies have not yet been conducted to search for effective antiviral agents against type I FIPV.…”

Section: Discussionmentioning

confidence: 99%

Identification of the peptide derived from S1 domain that inhibits type I and type II feline infectious peritonitis virus infection

et al. 2015

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Feline infectious peritonitis virus (FIPV) can cause a lethal disease in cats, feline infectious peritonitis (FIP). A therapeutic drug that is effective against FIP has not yet been developed. Peptides based on viral protein amino acid sequences have recently been attracting attention as new antiviral drugs. In the present study, we synthesized 30 overlapping peptides based on the amino acid sequence of the S1 domain of the type I FIPV strain KU-2 S protein, and investigated their inhibitory effects on FIPV infection. To evaluate the inhibitory effects on type I FIPV infection of these peptides, we investigated a method to increase the infection efficiency of poorly replicative type I FIPV. The efficiency of type I FIPV infection was increased by diluting the virus with medium containing a polycation. Of the 30 peptides, I-S1-8 (S461-S480), I-S1-9 (S471-S490), I-S1-10 (S481-S500), I-S1-16 (S541-S560), and I-S1-22 (S601-S620) significantly decreased the infectivity of FIPV strain KU-2 while I-S1-9 and I-S1-16 exhibited marked inhibitory effects on FIPV infection. The inhibitory effects on FIPV infection of these 2 peptides on other type I and type II FIPV strains, feline herpesvirus (FHV), and feline calicivirus (FCV) were also examined. These 2 peptides specifically inhibited type I and type II FIPV, but did FHV or FCV infection. In conclusion, the possibility of peptides derived from the S protein of type I FIPV strain KU-2 as anti-FIPV agents effective not only for type I, but also type II FIPV was demonstrated in vitro.

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“…The viruses were then added to FCWF-4 cells showing 70% confluency in a 96-well plate, and incubated for 48 h, until the development of cytopathic effect. Neutralising activity was determined by end-point dilution (Shiba et al, 2007).…”

Section: Virus Neutralisation Assaymentioning

confidence: 99%

Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats

Bálint

Farsang

Szeredi

et al. 2014

Veterinary Microbiology

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Feline infectious peritonitis virus (FIPV) is a major pathogen of Felidae. Despite the extensive efforts taken in the past decades, development of the "ideal" live attenuated FIPV vaccine was not successful yet. In the present study, we provide data of immunisation experiments with a recombinant FCoV pair differing only in the truncation (PBFIPV-DF-2) or completion (PBFIPV-DF-2-R3i) of their ORF3abc regions. In our previous in vivo studies, these viruses proved to show the characters of low virulent or avirulent FCoV phenotypes, respectively. Therefore, we hypothesised the ability of these viruses, as possible vaccine candidates, in conferring protection in specific pathogen free (SPF) Domestic Shorthair as well as in conventional purebred British Shorthair cats. In SPF cats, after two oronasal and two intramuscular vaccinations with two weeks intervals, both vaccine candidates provided 100% protection against lethal homologous challenge with the highly virulent FIPV DF-2 strain. In contrast, the conventional purebred British Shorthair cats did not develop protection when they were immunised with the same vaccination regimes. In these groups 100% of the PBFIPV-DF-2-R3i immunised animals developed antibody-dependent enhancement (ADE). Prolonged survival was observed in 40% of the animals, while 60% showed fulminant disease course. Genetic and more probably immunological differences between the SPF and non-SPF purebred kittens can explain the different outcome of the vaccination experiment. Our data highlight the diverse immune responses between SPF and conventional cats and suggest a decisive role of previous infection by heterologous causative agents in the outcome of the vaccination against FIP.

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Differentiation of feline coronavirus type I and II infections by virus neutralization test

Cited by 50 publications

References 21 publications

Genetic diversity and correlation with feline infectious peritonitis of feline coronavirus type I and II: A 5-year study in Taiwan

Genetic diversity and correlation with feline infectious peritonitis of feline coronavirus type I and II: A 5-year study in Taiwan

Identification of the peptide derived from S1 domain that inhibits type I and type II feline infectious peritonitis virus infection

Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats

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