Differentiation of Genomic Structure by rep-PCR Fingerprinting to Rapidly ClassifyXanthomonas campestrispv.vesicatoria

Louws, Frank J.; Fulbright, D. W.; Stephens, C. T.; Bruijn, Frans J. de

doi:10.1094/phyto-85-528

Cited by 114 publications

(79 citation statements)

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“…Use of the ERIC/REP-PCR was originally reported for genomic fingerprints of phytopathogenic bacteria [8]. In the recent years, ERIC/REP-PCR is also being used do detect genetic variability among fungal pathogens of several crops [9,10].…”

Section: Eric-and Rep-pcrmentioning

confidence: 99%

Identification of Phenotypic and Genotypic Variability among the Isolates of Ramularia areola of Brazilian Cotton

Girotto¹,

Marangoni²,

Matos³

et al. 2013

AJPS

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Genotypic and phenotypic variation among 16 isolates of Ramularia areola of Gossypium hirsutum collected from five different geographical regions of Brazil was studied through virulence spectrum on three cultivars in the glasshouse and through ERIC-and REP-PCR and ITS1-5.8S-ITS2 rDNA analysis. Difference in virulence spectrum and molecular analysis of some isolates was observed. ERIC-and REP-PCR showed similar results and revealed a high level of diversity among the isolates. A unique profile for both ERIC and REP was obtained for most isolates. On the other hand, the ITS rDNA analysis did not show different PCR-RFLP patterns. While some isolates differed among each other considering genotypic and phenotypic reactions, no clear cut evidence was found about the existence of genetic lineages of R. areola in Brazil. Identification of genetic variability among the R. areola isolates originated from different geographic regions would permit screening of Brazilian germplasm and achieve sources with a wide spectrum of resistance. This is the first report of the genotypic and phenotypic variability among the R. areola isolates originated from five cotton growing regions of Brazil.

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Section: Eric-and Rep-pcrmentioning

confidence: 99%

Identification of Phenotypic and Genotypic Variability among the Isolates of Ramularia areola of Brazilian Cotton

Girotto¹,

Marangoni²,

Matos³

et al. 2013

AJPS

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“…The utility of the repetitive BOX and ERIC sequences for characterization of plant-pathogenic bacteria has been widely demonstrated (12,13,16,18). PCR conditions described in other studies did not generate enough variability to discriminate among Xanthomonas strains of the same pathotype.…”

Section: Vol 68 2002 Relationship Among Xanthomonas Strains 1261mentioning

confidence: 99%

“…PCRs based on these repetitive sequences (rep-PCRs) have been used effectively for analysis of several genera and species of bacteria (12,14). For Xanthomonas species, rep-PCRs have been used to assess variation among pathovars and have revealed low levels of intrapathovar diversity (12,13,16,18,23).…”

mentioning

confidence: 99%

Genetic Relationship among Worldwide Strains of Xanthomonas Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR

Cubero

Graham

2002

Appl Environ Microbiol

154

166

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Partial sequence analysis of the ribosomal operon in Xanthomonas axonopodis allowed discrimination among strains causing the A, B, and C types of citrus bacterial canker (CBC) and quantification of the relationship of these organisms with other species and pathovars in the same genus. Sets of primers based on sequence differences in the internally transcribed spacer and on a sequence from the plasmid gene pthA involved in virulence were designed for specific identification of xanthomonads causing CBC diseases. The two sets were validated with a collection of Xanthomonas strains associated with citrus species. The primer set based on ribosomal sequences had a high level of specificity for X. axonopodis pv. citri, whereas the set based on the pthA gene was universal for all types of CBC organisms. Moreover, the relationships among worldwide Xanthomonas strains causing CBC were analyzed by amplification of repetitive sequences (enterobacterial repetitive intergenic consensus and BOX elements). Under specific conditions, pathotypes of these Xanthomonas strains could be discerned, and subgroups of the pathotypes were identified. Subgroups of strains were associated with certain geographic areas of the world, and on this basis the origin of type A strains introduced into Florida could be inferred.Xanthomonas axonopodis pv. citri and X. axonopodis pv. aurantifolii cause citrus bacterial canker (CBC), a serious disease of most citrus species and cultivars in many citrus-producing areas worldwide (1). Distinct forms of CBC based on different pathogenicities of the bacteria have been described. The Asiatic form or A type, caused by X. axonopodis pv. citri, is the most virulent form and affects the widest range of hosts, including Citrus spp. and many closely related rutaceous plants. The CBC B and C types are caused by X. axonopodis pv. aurantifolii. Pathotype B strains affect primarily lemon (Citrus limon (L.) Burm f.), but they also affect some other citrus species and are found only in Argentina and neighboring Uruguay and Paraguay. Pathotype C strains have been isolated in a small area of Sao Paulo State in Brazil and infect Key or Mexican lime (Citrus aurantifolia (Christm.) Swingle) (21). The symptoms induced by the three forms of canker organisms are similar and consist of erumpent and corky lesions surrounded by oily or water-soaked margins on leaf, fruit, and stem tissues (1). A new strain of X. axonopodis pv. citri, designated A*, was identified in southwest Asia and has a host range restricted naturally to Key or Mexican lime; this strain is closely related to type A strains (24). Another strain (type A w ), which behaves similarly, has recently been discovered in Florida; this strain has a restricted host range that includes Key or Mexican lime and alemow (Citrus macrophylla Wester)

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“…A diferenciação das espécies que formam o complexo da mancha bacteriana pode ser realizada através de ensaios bioquímicos (atividade amilolítica), patogenicidade em tomate e pimentão, mas com maior eficiência e rapidez por meio de técnicas moleculares, rep-PCR ou PCR com iniciadores específicos (Louws et al, 1995;Bouzar et al, 1999;Cuppels et al, 2006;Koenraadt et al, 2009;Pereira et al, 2011). A técnica de rep-PCR com o iniciador BOX apresenta vantagens por ser rápida, de fácil execução e altamente discriminatória para espécies de Xanthomonas.…”

unclassified

“…Já foi relatada alta diversidade dentro do grupo B de Xanthomonas causador da mancha bacteriana, aqui tratado como X. vesicatoria, a partir de perfis gerados por rep-PCR (Louws et al, 1995). Nenhum isolado foi identificado como X. euvesicatoria.…”

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Ocorrência e caracterização do complexo de espécies causadoras da mancha bacteriana do tomateiro no Alto Vale do Rio do Peixe, SC

Costa¹,

Araújo²,

Becker³

et al. 2012

Trop. plant pathol.

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RESUMOO objetivo deste trabalho foi identificar isolados de Xanthomonas causadores da mancha bacteriana do tomateiro para consumo in natura, provenientes da região do Alto Vale do Rio do Peixe, SC, Brasil, bem como caracterizá-los quanto à sensibilidade in vitro ao cobre. As espécies foram determinadas por similaridades de perfis genômicos gerados por BOX-PCR e a sensibilidade ao cobre foi estabelecida utilizando-se o meio CYE suplementado com sulfato de cobre nas concentrações de 50, 100 e 200 μg/mL. Do total de 44 isolados, 80% foram identificados como X. gardneri, 11% como X. perforans e 9% como X. vesicatoria. De acordo com a reação ao cobre, os isolados foram agrupados em quatro classes (S, sensíveis; MS, moderadamente sensíveis; MI, moderadamente insensíveis; I, insensíveis). Quanto à sensibilidade ao cobre, 98% de todos os isolados foram sensíveis a 200 μg/mL, sugerindo que aparentemente, a dosagem recomendada de produtos à base de cobre em campo, 10 vezes maior que a dosagem limítrofe utilizada nos testes in vitro, ainda é eficiente para as diferentes espécies da bactéria. Palavras-chave: Xanthomonas euvesicatoria, X. gardneri, X. perforans, X. vesicatoria, BOX-PCR, cobre, controle químico. ABSTRACT Occurrence and characterization of the species complex causing tomato bacterial spot in "Alto Vale do Rio do Peixe", SC, BrazilThe aim of this study was to identify at the species level Xanthomonas strains causing tomato bacterial spot in the region of "Alto Vale do Rio do Peixe", state of Santa Catarina, Brazil, as well as to determine their in vitro sensitivity to copper. Species were determined by similarity analysis of genomic profiles generated by BOX-PCR and sensitivity to copper was established using the CYE medium supplemented with copper sulfate at concentrations of 50, 100 and 200 μg/mL. Of the 44 isolates, 80% were identified as X. gardneri, 11% as X. perforans and 9% as X. vesicatoria. According to the response to copper, the isolates were divided into four classes (S, sensitive; MS, moderately sensitive; MI, moderately insensitive; I, insensitive). Regarding the sensitivity to copper, 98% of all isolates were sensitive at 200 μg/mL, suggesting that the recommended dosage of copper-based products registered for the crop may still provide effective control of the different bacterial species.

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Differentiation of Genomic Structure by rep-PCR Fingerprinting to Rapidly ClassifyXanthomonas campestrispv.vesicatoria

Cited by 114 publications

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Identification of Phenotypic and Genotypic Variability among the Isolates of <i>Ramularia areola</i> of Brazilian Cotton

Identification of Phenotypic and Genotypic Variability among the Isolates of <i>Ramularia areola</i> of Brazilian Cotton

Genetic Relationship among Worldwide Strains of Xanthomonas Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR

Ocorrência e caracterização do complexo de espécies causadoras da mancha bacteriana do tomateiro no Alto Vale do Rio do Peixe, SC

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Differentiation of Genomic Structure by rep-PCR Fingerprinting to Rapidly ClassifyXanthomonas campestrispv.vesicatoria

Cited by 114 publications

References 0 publications

Identification of Phenotypic and Genotypic Variability among the Isolates of &lt;i&gt;Ramularia areola&lt;/i&gt; of Brazilian Cotton

Identification of Phenotypic and Genotypic Variability among the Isolates of &lt;i&gt;Ramularia areola&lt;/i&gt; of Brazilian Cotton

Genetic Relationship among Worldwide Strains of Xanthomonas Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR

Ocorrência e caracterização do complexo de espécies causadoras da mancha bacteriana do tomateiro no Alto Vale do Rio do Peixe, SC

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Identification of Phenotypic and Genotypic Variability among the Isolates of <i>Ramularia areola</i> of Brazilian Cotton

Identification of Phenotypic and Genotypic Variability among the Isolates of <i>Ramularia areola</i> of Brazilian Cotton