Differentiation of human dermal fibroblasts towards endothelial cells

Junker, Johan P.E.; Lönnqvist, Susanna; Rakar, Jonathan; Karlsson, Lisa; Grenegård, Magnus; Kratz, Gunnar

doi:10.1016/j.diff.2013.01.005

Cited by 12 publications

(12 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…19 Human fibroblasts are abundant in the dermis and can be easily obtained from minimally invasive skin biopsies. 20,21 Considering their location within the dermis, fibroblasts are divided into papillary (superficial dermis) and reticular (deep dermis), exhibiting different characteristics in terms of cell morphology, production of ECM and growth factors, among others. 22,23 Although no specific markers distinguish both types of fibroblasts, differences in gene expression patterns exist, with reticular fibroblasts exhibiting an increased expression of genes involved in cell motility and contraction, including calponin-1 and transglutaminase-2 (TG2), whereas papillary fibroblasts characteristically express genes involved in the immune response, such as netrin-1 and podoplanin (PDPN).…”

mentioning

confidence: 99%

Fibroblast-Endothelial Partners for Vascularization Strategies in Tissue Engineering

Costa‐Almeida

Gómez-Lázaro

Ramalho

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

Cell-based approaches have emerged as a promising therapy to achieve successful vascularization in tissue engineering. Since fibroblasts activation and migration is required for physiological events relying on angiogenesis, we hypothesize herein that different fibroblasts exhibit distinct capacity to promote capillary-like structures assembly, by mature and progenitor endothelial cells (ECs). Outgrowth endothelial cells (OECs) were isolated from human umbilical cord blood samples and characterized by immunofluorescence and imaging flow cytometry for endothelial markers. Coculture systems were established using either human umbilical vein ECs (HUVECs) or OECs with fibroblasts, being evaluated at 7, 14, and 21 days of culture. Two types of human dermal fibroblasts (HDF) were used, namely neonatal human foreskin fibroblasts-1 (HFF-1) and juvenile HDF. OECs expressed EC markers and formed capillary-like structures. HFF-1 exhibited higher expression of transglutaminase-2, while HDF exhibited a higher expression of a-smooth muscle actin (a-SMA) and podoplanin, which were not observed for HFF-1. Formation of capillary-like structures was only observed in cocultures with HDF and not with HFF-1. No significant differences were found between HDF and OECs or HUVECs cocultures. These findings suggest that HDF is a preferential cell source for promoting vascularization, either using mature or progenitor ECs, probably due to their higher expression of a-SMA and podoplanin, and increased synthesis of extracellular matrix. This work opens new research possibilities regarding the use of specific fibroblast populations cocultured with ECs, as efficient partners for vascular development in regenerative medicine strategies.

show abstract

mentioning

confidence: 99%

Fibroblast-Endothelial Partners for Vascularization Strategies in Tissue Engineering

Costa‐Almeida

Gómez-Lázaro

Ramalho

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…The effects of ADSCs on the biological functions of fibroblasts and the secretion of ECMs through secretion have been studied and confirmed [ 51 ]. Fibroblasts and ADSCs have the ability to differentiate into adipocytes and hemangioendothelial cells and support their growth [ [52] , [53] , [54] , [55] , [56] ]. Our study demonstrated that when ADSCs were co-cultured with fibroblasts, adipogenic differentiation and hemangioendothelial cells were enhanced compared to when the two types of cells were cultured separately.…”

Section: Discussionmentioning

confidence: 99%

Study on promoting the regeneration of grafted fat by cell-assisted lipotransfer

Dong

2023

Regenerative Therapy

View full text Add to dashboard Cite

“…The introduction of cDNAs or CRISPR/Cas9 systems for gene editing can be achieved by either viral systems or non-viral/non-integration systems (85). In the context of vascular MPS, several groups have shown success in transdifferentiating human fibroblast cells to ECs (80,82,83,86,87) and SMCs (88) or ECs to SMCs (89).…”

Section: Cell Sourcementioning

confidence: 99%

Vascular microphysiological systems to model diseases

Zhang

Truskey

2020

Cell Gene Therapy Insights

View full text Add to dashboard Cite

Human vascular microphysiological systems (MPS) represent promising three-dimensional in vitro models of normal and diseased vascular tissue. These systems build upon advances in tissue engineering, microfluidics, and stem cell differentiation and replicate key functional units of organs and tissues. Vascular models have been developed for the microvasculature as well as medium-size arterioles. Key functions of the vascular system have been reproduced and stem cells offer the potential to model genetic diseases and population variation in genes that may increase individual risk for cardiovascular disease. Such systems can be used to evaluate new therapeutics options.

show abstract

Differentiation of human dermal fibroblasts towards endothelial cells

Cited by 12 publications

References 60 publications

Fibroblast-Endothelial Partners for Vascularization Strategies in Tissue Engineering

Fibroblast-Endothelial Partners for Vascularization Strategies in Tissue Engineering

Study on promoting the regeneration of grafted fat by cell-assisted lipotransfer

Vascular microphysiological systems to model diseases

Contact Info

Product

Resources

About