Differentiation of human mesenchymal stem cells (MSC) to dopaminergic neurons: A comparison between Wharton’s Jelly and olfactory mucosa as sources of MSCs

Conductive hydrogel based on chitosan-aniline pentamer/gelatin/agarose significantly promoted motor neuron-like cells differentiation of human olfactory ecto-mesenchymal stem cells

“…It was demonstrated that the scaffold conductivity plays an important role for neural tissue engineering and could significantly increase neuronal gene expression without any other external stimuli [33]. In this study, OE-MSCs were used as a new meso-ectodermal cell source for motor neuron-like cell differentiation and we assume that OE-MSCs, which are 100% nestin-positive [63,64], have better tendency to differentiate into neuron cells compared to MSCs. OE-MSCs were seeded in the presence of neural inductive molecules on scaffolds and TCP plates for 2 weeks and the characterization of differentiated cells performed via Real-time PCR, immunostaining, and flow cytometry.…”

Olfactory Mucosa Mesenchymal Stem Cells Ameliorate Cerebral Ischemic/Reperfusion Injury Through Modulation of UBIAD1 Expression

“…Third, OM-MSCs derived from the neural crest, which maintains in a condition of embryo-like development. OM-MSCs have higher proliferation and capacity of differentiation into dopaminergic neurons (Alizadeh et al, 2019a), which serves as an available candidate for the treatment of neurological diseases. Fourth, the human olfactory mucosa is a kind of permanently self-renewing tissue, which has various cells sustaining its normal function and regeneration (Lindsay et al, 2010).…”

Human Mesenchymal Stromal Cells Unveil an Unexpected Differentiation Potential toward the Dopaminergic Neuronal Lineage

“…Among adult stem cells, some results were observed using cells that have a neuroectodermal origin, such as neural progenitor cells and olfactory ectomesenchymal stem cells. However, these cells present important limitations related to their collection, availability, and differentiative potential: indeed, although some DA markers become detectable after the differentiation process, they do not acquire the morphology of primary DA neurons, lacking the typical long and branched neurites [20][21][22]. Analyzing the landscape of human The immunofluorescence data were also confirmed by Western blot analysis, which evidenced a comparable expression of the three DA markers between the hFM-MSCs-derived DA neuron-like cells and the hiPSCs-derived DA neurons (used as positive controls) ( Figure S2).…”

“…Among adult stem cells, some results were observed using cells that have a neuroectodermal origin, such as neural progenitor cells and olfactory ectomesenchymal stem cells. However, these cells present important limitations related to their collection, availability, and differentiative potential: indeed, although some DA markers become detectable after the differentiation process, they do not acquire the morphology of primary DA neurons, lacking the typical long and branched neurites [20][21][22]. Analyzing the landscape of human perinatal stem cells, it has been reported that c-KIT + /OCT4 + human amniotic fluid stem cells exposed to differentiative conditions acquired a neuron-like phenotype and, transplanted into a rat model, survived up to 6 months [23]; despite these encouraging findings, Donaldson et al reported that human amniotic fluid stem cells were not able to differentiate into DA neurons in vitro [24].…”