Differentiation of Human Monocytes In Vitro: A Model of Macrophage Ontogeny

Andreesen, Reinhard; Kreutz, Marina

doi:10.1007/978-3-662-03011-0_2

Cited by 5 publications

(4 citation statements)

References 64 publications

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“…Monocytes from the bone marrow enter the peripheral blood, where they circulate for 2–3 days [ 7 ], before passing into the tissues and converting into macrophages—present in the connective tissue (histiocytes), liver (Kupffer cells), spleen, lungs (alveolar macrophages) and central nervous system (microglial cells) [ 8 ]. The percentage of monocytes does not exceed 10% of the total number of white blood cells.…”

Section: Introductionmentioning

confidence: 99%

Phage-Phagocyte Interactions and Their Implications for Phage Application as Therapeutics

Jończyk‐Matysiak

Weber‐Dąbrowska

Owczarek

et al. 2017

Viruses

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Phagocytes are the main component of innate immunity. They remove pathogens and particles from organisms using their bactericidal tools in the form of both reactive oxygen species and degrading enzymes—contained in granules—that are potentially toxic proteins. Therefore, it is important to investigate the possible interactions between phages and immune cells and avoid any phage side effects on them. Recent progress in knowledge concerning the influence of phages on phagocytes is also important as such interactions may shape the immune response. In this review we have summarized the current knowledge on phage interactions with phagocytes described so far and their potential implications for phage therapy. The data suggesting that phage do not downregulate important phagocyte functions are especially relevant for the concept of phage therapy.

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Section: Introductionmentioning

confidence: 99%

Phage-Phagocyte Interactions and Their Implications for Phage Application as Therapeutics

Jończyk‐Matysiak

Weber‐Dąbrowska

Owczarek

et al. 2017

Viruses

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“…Further, GG leachates did not activate the monocytes, as no TNFα and IL6 were released. This suggests that there are insufficient pyrogen residues within the GG material for the activation of monocytes [ 52 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

Gellan Gum Is a Suitable Biomaterial for Manual and Bioprinted Setup of Long-Term Stable, Functional 3D-Adipose Tissue Models

Albrecht

Dolderer

Nellinger

et al. 2022

Gels

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Due to its wide-ranging endocrine functions, adipose tissue influences the whole body’s metabolism. Engineering long-term stable and functional human adipose tissue is still challenging due to the limited availability of suitable biomaterials and adequate cell maturation. We used gellan gum (GG) to create manual and bioprinted adipose tissue models because of its similarities to the native extracellular matrix and its easily tunable properties. Gellan gum itself was neither toxic nor monocyte activating. The resulting hydrogels exhibited suitable viscoelastic properties for soft tissues and were stable for 98 days in vitro. Encapsulated human primary adipose-derived stem cells (ASCs) were adipogenically differentiated for 14 days and matured for an additional 84 days. Live-dead staining showed that encapsulated cells stayed viable until day 98, while intracellular lipid staining showed an increase over time and a differentiation rate of 76% between days 28 and 56. After 4 weeks of culture, adipocytes had a univacuolar morphology, expressed perilipin A, and secreted up to 73% more leptin. After bioprinting establishment, we demonstrated that the cells in printed hydrogels had high cell viability and exhibited an adipogenic phenotype and function. In summary, GG-based adipose tissue models show long-term stability and allow ASCs maturation into functional, univacuolar adipocytes.

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“…Differentiation from MO to MAC was concomitant with an increased TNF‐α and M‐CSF secretion ( Andreesen & Kreutz, 1994). In this study MO‐derived MAC after rhuGM‐CSF treatment did not reach the same secretory competence when compared to pretreatment values, suggesting activation of the circulating MO, which is known to impair MO to MAC differentiation ( Brugger et al , 1991 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of granulocyte‐macrophage colony‐stimulating factor treatment on phenotype, cytokine release and cytotoxicity of circulating blood monocytes and monocyte‐derived macrophages

et al. 1998

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We studied phenotype, function and differentiation of mononuclear phagocytes in 11 cancer patients treated subcutaneously with 10O microg/kg recombinant human (rhu) GM-CSF for 7 d. The rhuGM-CSF treatment induced (1) a 5.9-fold increase in the number of blood monocytes (MO), (2) a decrease of CD14bright/CD16bright cells with a diminution of the mean fluorescence intensity (MFI) of CD14, and (3) a decrease of MO cellular cytotoxicity. In patients' sera, tumour necrosis factor (TNF)-alpha, interleukin (IL)-10, IL-12, neopterin, macrophage-colony-stimulating factor (M-CSF), and IL-1 receptor antagonist (IL-1RA) increased, whereas GM-CSF and granulocyte-colony-stimulating factor (G-CSF) decreased after an initial peak. In whole blood samples the lipopolysaccharide (LPS)-stimulated release of TNF-alpha, IL-6 and IL-1RA increased initially, whereas IL-1beta, IL-10 and IL-12 decreased. During differentiation from MO to macrophages (MAC), interferon (IFN)-gamma-stimulated tumour cytotoxicity increased, but both MO and MAC were less cytotoxic upon rhuGM-CSF treatment. The differentiation-associated increase of LPS-induced TNF-alpha, IL-1RA and IL-10 secretion was reduced by the rhuGM-CSF treatment, and the expression of CD14 on MAC as well as the proportion of CD14+/CD16+, CD14+/MAX.1+ and CD14+/CD71+ cells in 7-d cultured MAC declined. We interpret these findings as (1) an increase of immature MO upon rhuGM-CSF therapy, (2) a priming effect on the LPS-induced proinflammatory cytokine repertoire of MO, and (3) an impact of rhuGM-CSF on the capacity of MO to differentiate to MAC in vitro.

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Differentiation of Human Monocytes In Vitro: A Model of Macrophage Ontogeny

Cited by 5 publications

References 64 publications

Phage-Phagocyte Interactions and Their Implications for Phage Application as Therapeutics

Phage-Phagocyte Interactions and Their Implications for Phage Application as Therapeutics

Gellan Gum Is a Suitable Biomaterial for Manual and Bioprinted Setup of Long-Term Stable, Functional 3D-Adipose Tissue Models

Effect of granulocyte‐macrophage colony‐stimulating factor treatment on phenotype, cytokine release and cytotoxicity of circulating blood monocytes and monocyte‐derived macrophages

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