Differentiation of Mouse Primordial Germ Cells into Functional Oocytes In Vitro

Morohaku, Kanako; Hirao, Yuji; Obata, Yayoi

doi:10.1007/s10439-017-1815-7

Cited by 11 publications

(8 citation statements)

References 109 publications

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“…Using a stereomicroscope, we dissected the embryos and collected the gonads attached to the mesonephros, which were removed from the gonad using tungsten needles just before the seeding of the gonad. Our culture method for the ovary was based on the protocol described previously by Morahaku et al 26 , 72 . However, in order to perform in-depth cytological, in situ immunofluorescence, and mitochondrial activity studies, we modified the method of the culture procedure depending on the process to be evaluated—follicular progression, immunofluorescence, or mitochondrial activity.…”

Section: Methodsmentioning

confidence: 99%

The antiandrogenic vinclozolin induces differentiation delay of germ cells and changes in energy metabolism in 3D cultures of fetal ovaries

González-Sanz

Barreñada

Rial

et al. 2020

Sci Rep

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Vinclozolin is a pesticide with antiandrogenic activity as an endocrine disruptor compound. Its effects upon the progression of primordial follicles were assessed in cultures of mouse fetal ovaries from the onset of meiotic differentiation of germ cells (13.5 days post coitum) and from both in vivo exposed mice and in vitro exposed ovaries. Exposure of ovaries to vinclozolin—at in vitro dosages ranging from 10 to 200 μM and in 3D ex vivo culture following in vivo exposure to 50 mg/kg bw/day—showed delays in meiocyte differentiation and in follicle growth, even at the lowest in vitro dose exposure. Immunofluorescent analysis showed the presence of the proteins MSY2 and NOBOX in the primary follicles but no difference in the level of protein signals or in the number of follicles in relation to treatment. However, assessing the cytological differentiation of germ cells by detecting the synaptonemal complex protein SYCP3, the exposure to vinclozolin delayed meiotic differentiation from both in vitro- and in vivo-exposed ovaries. These effects were concomitant with changes in the energy metabolism, detected as a relative increase of glycolytic metabolism in live-cell metabolic assays in exposed ovaries.

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Section: Methodsmentioning

confidence: 99%

The antiandrogenic vinclozolin induces differentiation delay of germ cells and changes in energy metabolism in 3D cultures of fetal ovaries

González-Sanz

Barreñada

Rial

et al. 2020

Sci Rep

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“…The following issues need to be resolved ( i ) hES cells need to be derived by somatic cell nuclear transfer for providing biological parenthood, ( ii ) iPS cells have inherent drawbacks including high chances that they may harbour genomic as well as mitochondrial mutations and also at times they may remain partially reprogrammed and retain the epigenetic marks of the somatic cells from which they are derived, ( iii ) methods need to be evolved to convert hES/iPS cells into primordial germ cells-like cells (PGCLCs) since PGCs are natural precursors for gametes, and ( iv ) hES cells are in a ‘primed’ state and need to be converted to ‘naïve’ state to improve differentiation into gametes. This may expand their differentiation/regenerative ability 17 18 25 26 27 28 .…”

Section: Available Options For Fertility Preservation Before Oncothermentioning

confidence: 99%

Stem cells survive oncotherapy & can regenerate non-functional gonads

Bhartiya¹

2018

Indian Journal of Medical Research

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A large proportion of patients who survive cancer are rendered infertile as an unwanted side effect of oncotherapy. Currently accepted approaches for fertility preservation involve banking eggs/sperm/embryos or ovarian/testicular tissue before oncotherapy for future use. Such approaches are invasive, expensive, technically challenging and depend on assisted reproductive technologies (ART). Establishing a gonadal tissue bank (for cancer patients) is also fraught with ethical, legal and safety issues. Most importantly, patients who find it difficult to meet expenses towards cancer treatment will find it difficult to meet expenses towards gonadal tissue banking and ART to achieve parenthood later on. In this review an alternative strategy to regenerate non-functional gonads in cancer survivors by targeting endogenous stem cells that survive oncotherapy is discussed. A novel population of pluripotent stem cells termed very small embryonic-like stem cells (VSELs), developmentally equivalent to late migratory primordial germ cells, exists in adult gonads and survives oncotherapy due to their quiescent nature. However, the stem-cell niche gets compromised by oncotherapy. Transplanting niche cells (Sertoli or mesenchymal cells) can regenerate the non-functional gonads. This approach is safe, has resulted in the birth of fertile offspring in mice and could restore gonadal function early in life to support proper growth and later serve as a source of gametes. This newly emerging understanding on stem cells biology can obviate the need to bank gonadal tissue and fertility may also be restored in existing cancer survivors who were earlier deprived of gonadal tissue banking before oncotherapy.

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“…However, there have been no subsequent reports on the production of porcine chimeras. Most iPSC lines fulfilled the criteria of pluripotency, but the cells could not contribute to chimeras or generate cloned piglets [56][57][58][59][60] .…”

Section: Derivation Of Pluripotent Stem Cells From Primordial Germ Cellsmentioning

confidence: 99%

Porcine pluripotent stem cells: progress, challenges and prospects

Han¹,

Miao²,

Hua³

et al. 2019

Front. Agr. Sci. Eng.

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Pluripotent stem cells (PSCs) are characterized by their capacity for high self-renewal and multiple differentiation potential and include embryonic stem cells, embryonic germ cells and induced PSCs. PSCs provide a very suitable model for the studies of human diseases, drugs screening, regenerative medicine and developmental biology research. Pigs are considered as an ideal model for preclinical development of human xenotransplantation, therapeutic approaches and regenerative medicine because of their size and physiological similarity to humans. However, lack of knowledge about the derivation, characterization and pluripotency mechanisms of porcine PSCs hinders progress in these biotechnologies. In this review, we discuss the latest progress on porcine PSCs generation, evaluation criteria for pluripotency, the scientific and technical questions arising from these studies. We also introduce our perspectives on porcine PSC research, in the hope of providing new ideas for generating naive porcine PSCs and animal breeding.

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Differentiation of Mouse Primordial Germ Cells into Functional Oocytes In Vitro

Cited by 11 publications

References 109 publications

The antiandrogenic vinclozolin induces differentiation delay of germ cells and changes in energy metabolism in 3D cultures of fetal ovaries

The antiandrogenic vinclozolin induces differentiation delay of germ cells and changes in energy metabolism in 3D cultures of fetal ovaries

Stem cells survive oncotherapy & can regenerate non-functional gonads

Porcine pluripotent stem cells: progress, challenges and prospects

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