Differentiation of skeletal muscle cells in culture.

Inestrosa, Nibaldo C.

doi:10.1247/csf.7.91

Cited by 18 publications

(5 citation statements)

References 138 publications

(95 reference statements)

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“…In agreement with this interpretation, the slower Ca uptake response of myoblasts to the sterol as compared with differentiated skeletal muscle (Fig. 6) may be related to the fact that functionally competent Ca channels appear at a later stage of development of the cells (100,101). Evidence suggesting an effect of 1,25-(OH) 2 D on Ca fluxes through Ca channels in tissues other than muscle has been obtained (102)(103)(104).…”

Section: Genome-independent Effects Of 125-(oh) 2 Dsupporting

confidence: 67%

Role of Vitamin D in Skeletal Muscle Function*

1986

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Section: Genome-independent Effects Of 125-(oh) 2 Dsupporting

confidence: 67%

Role of Vitamin D in Skeletal Muscle Function*

1986

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“…, 15 The greatest maturation of these newly formed fibres occurs later, around one week after plating 16 . It is possible to determine each phase of cell differentiation based on the transcription factors being expressed, 13 cell morphology, 16 , 17 and the expression of muscle-specific proteins on the cytoskeleton 18 . Importantly, primary muscle cell culture is a mixed culture composed of fibroblasts and muscle cells.…”

Section: Discussionmentioning

confidence: 99%

Generation of Yellow Fever virus vaccine in skeletal muscle cells of chicken embryos

Souza

Manso

Oliveira

et al. 2019

Mem. Inst. Oswaldo Cruz

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BACKGROUND The Yellow Fever (YF) vaccine is produced by the inoculation of embryonated chicken eggs with YF17DD virus on the ninth day of development. Full embryos are collected on the twelfth day of development for vaccine formulation. Skeletal muscle tissue is the main site where biosynthesis of viral particles occurs.OBJECTIVES This study aimed to analyse the experimental infection of skeletal muscle cells of chicken embryos by the 17DD Yellow Fever virus (YFV) in vivo and in vitro.METHODS Chicken embryos infected with YF17DD virus were analysed by immunofluorescence using confocal and super-resolution microscopes. Primary cultures of skeletal muscle cells of non-infected chicken embryos were evaluated for susceptibility and permissiveness to YF17DD virus using different protocols. This evaluation was performed based on morphological, viral titration, molecular biology, and colorimetric techniques.FINDINGS The present work phenotypically characterises embryonic chicken skeletal muscle cells as myogenic precursors expressing the Pax7 transcription factor in some cases. We demonstrated that these cells are susceptible to in vitro infection at different multiplicities of infection (MOIs), reproducing the same infection pattern observed in vivo. Furthermore, myogenic precursors and myoblasts are preferred infection targets, but establishment of infection does not depend on the presence of these cells. The peak of viral production occurred at 48 hpi, with decay occurring 72 hpi, when the cytopathic effect can be observed.MAIN CONCLUSIONS In conclusion, the primary culture of chicken skeletal muscle cells is a good model for studying muscle cells infected with YF17DD virus. This culture system displays satisfactory emulation of the in vitro phenomenon observed, contributing to our understanding of virus infection dynamics and leading to the development of alternative methods of vaccine production.

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“…Terminal differentiation of skeletal myoblasts, as evidenced by formation of multinuclear myotubes, involves density-dependent arrest of DNA synthesis, cellcell recognition and fusion of opposing plasma membranes [3][4][5][6][7]. While various researchers have implicated the cell surface in the events of myoblast differentiation, the actual mechanisms which mediate the transcellular surface interactions leading to membrane fusion remain to be resolved.…”

Section: Introductionmentioning

confidence: 99%

Glycosphingolipids during skeletal muscle cell differentiation: Comparison of normal and fusion-defective myoblasts

Cambron

Leskawa

1994

Mol Cell Biochem

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The regulation of glycosphingolipid (GSL) synthesis in culture by fusion-competent (E63) myoblasts and fusion-defective (fu-1) cells was examined. Upon reaching confluency E63 cells fused to form multinucleated myotubes and demonstrated many characteristics of developing skeletal muscle including induction of creatine kinase activity and a shift in creatine kinase isozymes to the MM isoform. The fu-1 cells displayed none of these characteristics, despite the fact that both cells were cloned from the same parental myoblast line (rat L8). There was a transient increase in the synthesis of total neutral GSLs by E63 cells at the time of membrane fusion. In contrast, neutral GSL synthesis by fu-1 cells gradually decreased with time in culture. The major GSLs synthesized by both cell types were lactosylceramide and ganglioside GM3, with more complex structures being observed with prolonged time in culture. Several glycosyltransferase activities were assayed at varying times in culture. Generally, the changes in activities fell into three groups. One group was maximally activated at the end of the culture period (GalT-3, GalNAcT-1 and GalT-6). Another group was maximally activated during the time of active membrane fusion (GlcT and SAT-1). A third group was maximally activated at the time of cell contact and the beginning of membrane fusion (GlcNAcT-1 and GalT-2). In terms of the times of maximal activation there were few differences between E63 and fu-1 cells, with one notable exception. The activity of GalT-2 (lactosylceramide synthase) in E63 cells increased dramatically upon contact and the beginning of membrane fusion, whereas there were no changes in GalT-2 activity in fu-1 cells during time in culture. These results support our hypothesis that membrane glycosphingolipids play an important role in the differentiation of skeletal muscle cells.

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Differentiation of skeletal muscle cells in culture.

Cited by 18 publications

References 138 publications

Role of Vitamin D in Skeletal Muscle Function*

Role of Vitamin D in Skeletal Muscle Function*

Generation of Yellow Fever virus vaccine in skeletal muscle cells of chicken embryos

Glycosphingolipids during skeletal muscle cell differentiation: Comparison of normal and fusion-defective myoblasts

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