Differentiation of Sterol-Requiring from Sterol-Nonrequiring Mycoplasmas by Amphotericin B

Rottem, S.

doi:10.1128/aem.23.3.659-660.1972

Cited by 8 publications

(1 citation statement)

References 6 publications

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“…Supporting information useful for mollicute classifications at all taxonomic levels may be obtained from tests other than those mandated herein. For example, sensitivities to rifampicin (Gadeau et al, 1986;Pellegrin et al, 1990;Gaurivaud et al, 1996), digitonin (Freundt et al, 1973;Tully, 1983c) or amphotericin B (Rottem, 1972) characterize some taxa. Tests that detect a-D-glucosidase (Williams & Wittler, 1971;, benzyl viologen reduction (Pollack et al, 1996a) or adsorption of erythrocytes to colonies on solid medium (Manchee & Taylor-Robinson, 1968;Aluotto et al, 1970;) are of interest in some species.…”

Section: (X) Additional Testsmentioning

confidence: 99%

Revised minimal standards for description of new species of the class Mollicutes (division Tenericutes)

Brown

Whitcomb

Bradbury

2007

International Journal of Systematic and Evolutionary Microbiology

138

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Minimal standards for novel species of the class Mollicutes (trivial term, mollicutes), last published in 1995, require revision. The International Committee on Systematics of Prokaryotes Subcommittee on the Taxonomy of Mollicutes proposes herein revised standards that reflect recent advances in molecular systematics and the species concept for prokaryotes. The mandatory requirements are: (i) deposition of the type strain into two recognized culture collections, preferably located in different countries; (ii) deposition of the 16S rRNA gene sequence into a public database, and a phylogenetic analysis of the relationships among the 16S rRNA gene sequences of the novel species and its neighbours; (iii) deposition of antiserum against the type strain into a recognized collection; (iv) demonstration, by using the combination of 16S rRNA gene sequence analyses, serological analyses and supplementary phenotypic data, that the type strain differs significantly from all previously named species; and (v) assignment to an order, a family and a genus in the class, with an appropriate specific epithet. The 16S rRNA gene sequence provides the primary basis for assignment to hierarchical rank, and may also constitute evidence of species novelty, but serological and supplementary phenotypic data must be presented to substantiate this. Serological methods have been documented to be congruent with DNA-DNA hybridization data and with 16S rRNA gene placements. The novel species must be tested serologically to the greatest extent that the investigators deem feasible against all neighbouring species whose 16S rRNA gene sequences show .0.94 similarity. The investigator is responsible for justifying which characters are most meaningful for assignment to the part of the mollicute phylogenetic tree in which a novel species is located, and for providing the means by which novel species can be identified by other investigators.

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