Differentiation of the follicle-associated epithelium in ileal Peyer?s patch and production of 50-nm particles are maintained in B-cell-depleted fetal sheep

Lie, Ki; Press, Charles McL.; McCullagh, Peter; McClure, S.J.; Landsverk, Thor

doi:10.1007/s00441-004-0977-4

Cited by 3 publications

(4 citation statements)

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“…The early differentiation of epithelial cells into FAE might be dependent on the early presence of B-cells [89]. However, the FAE in ileal PP of B-cell depleted fetal lambs showed a normal differentiation [60]. FAE and M-cells are very important for phagocytosis and transcytosis of external antigens [57].…”

Section: Follicle-associated Epithelium and Other Accessory Cells Of mentioning

confidence: 99%

“…Follicular dendritic cells that are reactive with 5' nucleotidase and Mg 2+ dependent adenosine triphosphatase are also seen in the ileal PP as well as in other primary lymphoid follicles in peripheral lymph nodes [43,75]. Depletion of Bcells from fetal lambs by anti IgM antibody injection generated PP follicles composed of clusters of FDC's [60,92]. Ileal PP follicles have also been used to generate a number of clones of mesenchymallyderived stromal cells [38,39].…”

Section: Stromal Cells In the Ileal And Jejunal Ppmentioning

confidence: 99%

“…The analysis of other accessory cells found in the ileal PP follicles has involved the use of the B-cell depleted fetal lambs described above [42,60,89,92]. The rudimentary follicles of ileal PP in these animals show strong reactivity for 5' nucleotidase, indicating the presence of follicular dendritic cells.…”

Section: Follicle-associated Epithelium and Other Accessory Cells Of mentioning

confidence: 99%

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The sheep and cattle Peyer’s patch as a site of B-cell development

et al. 2006

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-In sheep and cattle, the ileal Peyer's patch (PP), which extends one-two meters along the terminal small intestine, is a primary lymphoid organ of B-cell development. B-cell diversity in the ileal PP is thought to develop by combinatorial mechanisms, gene conversion and/or point mutation. These species also have jejunal PP that function more like secondary lymphoid tissues concerned with mucosal immune reactions. These two types of PP differ significantly in their histology, ontogeny and the extent of lymphocyte traffic. The prenatal development of follicles in the PP begins first in the jejunum during the middle of gestation and then in the ileum during late gestation. B-cells proliferate rapidly in the ileal PP follicle; up to five percent of these cells survive while the majority dies by apoptosis, perhaps driven by the influence of environmental antigen and/ or self-antigen. The surviving cells migrate from the ileal PP and populate the peripheral B-cell compartment. By adolescence, the ileal PP has involuted but the function of jejunal PP, compatible with a role as secondary lymphoid organ, continues throughout life. In this review, we focus on the development of PP as a site of B-cell repertoire generation, positive and negative B-cell selection, and the differences between ileal PP and jejunal PP.

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Section: Follicle-associated Epithelium and Other Accessory Cells Of mentioning

confidence: 99%

Section: Stromal Cells In the Ileal And Jejunal Ppmentioning

confidence: 99%

Section: Follicle-associated Epithelium and Other Accessory Cells Of mentioning

confidence: 99%

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The sheep and cattle Peyer’s patch as a site of B-cell development

et al. 2006

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“…This indicates that the signals for M cell differentiation vary between different anatomical compartments. In addition, previous studies of B cell-depleted fetal sheep showed that ileal PPs contain differentiated FAE and reticular cells [20,24]. Therefore, we speculate that development of the FAE in primary lymphoid organs is distinct from that of FAE in secondary lymphoid organs.…”

Section: Discussionmentioning

confidence: 59%

C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches

et al. 2013

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The follicle-associated epithelium (FAE) of Peyer's patches (PPs) contains M cells that are important for reducing mucosal immune responses by transporting antigens into the underlying lymphoid tissue. We generated a monoclonal antibody (C6) that reacted with the FAE of calf ileal PPs, and analyzed the characteristics of C6 using immunohistochemistry and Western blotting. FAE of the ileal PP was stained with C6 during both late fetal developmental and postnatal stages. Neither the villous epithelial cell nor intestinal crypt basal cells were stained at any developmental stage. During the prenatal stages, FAE of the jejunal PP was C6-negative. However, a few C6-positive cells were distributed diffusely in some FAE of the jejunal PPs during the postnatal stages. The protein molecular weight of the antigen recognized by C6 was approximately 45 kDa. These data show that C6 is useful for identifying the FAE in ileal PPs and further suggest that differentiation of the FAE in these areas is independent of external antigens.

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