Differentiation of Trypanosoma rangeli: high production of infective trypomastigote forms in vitro

Koerich, Leonardo B.; Emmanuelle-Machado, Priscilla; Santos, Kelly de Oliveira; Grisard, Edmundo C.; Steindel, Mário

doi:10.1007/s004360100501

Cited by 21 publications

(5 citation statements)

References 14 publications

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“…Other confounding factors in experimental design in later studies include insects that were periodically anesthetized for colony maintenance and problems related to infection route. Two studies reported augmented negative effects in inoculated insects (Watkins, unpublished doctoral dissertation, Koerich et al ) that were not observed in orally infected insects in the same experiment, suggesting problems stemming from the parasite injection procedure. Additionally, many studies that employed the oral infection technique did not take into account the quantity of blood consumed by each insect in the infective meal (Grewal , Tobie , Gómez , Añez , Añez et al ).…”

Section: Experimental Design and Presentation Of Resultsmentioning

confidence: 96%

What is the ‘true’ effect ofTrypanosoma rangelion its triatomine bug vector?

Peterson

Graham

2016

Journal of Vector Ecology

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The phrase, "T. rangeli is pathogenic to its insect vector, " is commonly found in peer-reviewed publications on the matter, such that it has become the orthodox view of this interaction. In a literature survey, we identified over 20 papers with almost the exact phrase and several others alluding to it. The idea is of particular importance in triatomine population dynamics and the study of vector-borne T. cruzi transmission, as it could mean that triatomines infected with T. rangeli have lower fitness than uninfected insects. Trypanosoma rangeli pathogenicity was first observed in a series of studies carried out over fifty years ago using the triatomine species Rhodnius prolixus. However, there are few studies of the effect of T. rangeli on its other vector species, and several of the studies were carried out with R. prolixus under non-physiological conditions. Here, we re-evaluate the published studies that led to the conclusion that T. rangeli is pathogenic to its vector, to determine whether or not this indeed is the "true" effect of T. rangeli on its triatomine vector.

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Section: Experimental Design and Presentation Of Resultsmentioning

confidence: 96%

What is the ‘true’ effect ofTrypanosoma rangelion its triatomine bug vector?

Peterson

Graham

2016

Journal of Vector Ecology

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“…Light microscopy was performed on Giemsa-stained smears using M1011, M1014, and M1015 parasites from culture with NNN and Schneider medium supplemented with 10% fetal bovine serum by three, 10, and 17 days. Additionally, epimastigote forms of the M1011 isolate were transferred at 1 × 10 6 parasites/mL into Roswell Park Memorial Institute (RPMI) medium with 5% fetal calf serum at pH 8.0 for differentiation and acquisition of trypomastigote forms, following a protocol described by Koerich et al [ 48 ]. The percentage of trypomastigote forms was determined by counting 100 randomly selected forms on the slides.…”

Section: Methodsmentioning

confidence: 99%

Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats

et al. 2020

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This study describes the morphological, biochemical, and molecular differences among Trypanosoma dionisii isolates from hemocultures of hematophagous (Desmodus rotundus; n = 2) and insectivorous (Lonchorhina aurita; n = 1) bats from the Atlantic Rainforest of Rio de Janeiro, Brazil. Fusiform epimastigotes from the hematophagous isolates were elongated, whereas those of the insectivorous isolate were stumpy, reflected in statistically evident differences in the cell body and flagellum lengths. In the hemocultures, a higher percentage of trypomastigote forms (60%) was observed in the hematophagous bat isolates than that in the isolate from the insectivorous bat (4%), which demonstrated globular morphology. Three molecular DNA regions were analyzed: V7V8 (18S rDNA), glycosomal glyceraldehyde 3-phosphate dehydrogenase gene, and mitochondrial cytochrome b gene. The samples were also subjected to multilocus enzyme electrophoresis and random amplified polymorphic DNA analysis. All isolates were identified as T. dionisii by phylogenetic analysis. These sequences were clustered into two separate subgroups with high bootstrap values according to the feeding habits of the bats from which the parasites were isolated. However, other T. dionisii samples from bats with different feeding habits were found in the same branch. These results support the separation of the three isolates into two subgroups, demonstrating that different subpopulations of T. dionisii circulate among bats.

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“…The trypomastigotes forms were obtained by cultivating epimastigotes in DMEM medium, pH 8 . 0 at 28 xC, according to Koerich et al (2002).…”

Section: Parasitesmentioning

confidence: 99%

Characterization of D-glucose transport in Trypanosoma rangeli

et al. 2006

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Like in other trypanosomatids D-glucose is a crucial source of energy to Trypanosoma rangeli, a non-pathogenic parasite that in Central and South America infects triatomine vectors and different mammalian species, including humans. In several trypanosome species, D-glucose transporters were already described and cloned. In this study, we characterized the D-glucose transport activity present in 2 life-stage forms of T. rangeli (epimastigotes and trypomastigotes) using D-[U-14C]glucose as substrate. Our results indicate that T. rangeli transports D-glucose with high affinity in both epimastigote (Km 30 microM) and trypomastigotes (Km 80 microM) life-forms. Both transport activities were inhibited by Cytochalasin B and Phloretin, indicating that probably D-glucose uptake in T. rangeli is mediated by facilitated diffusion of the sugar. Significant differences were observed between epimastigotes and trypomastigotes in relation to their affinity for D-glucose analogues, and the predicted amino acid sequence of a putative D-glucose transporter from T. rangeli (TrHT1) showed a larger identity with the T. cruzi D-glucose transporter encoded by the TcrHT1 gene than with other transporters already characterized in trypanosomatids.

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Differentiation of Trypanosoma rangeli: high production of infective trypomastigote forms in vitro

Cited by 21 publications

References 14 publications

What is the ‘true’ effect ofTrypanosoma rangelion its triatomine bug vector?

What is the ‘true’ effect ofTrypanosoma rangelion its triatomine bug vector?

Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats

Characterization of D-glucose transport in Trypanosoma rangeli

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