Differentiation of two morphologically similar Amazonian Aniba species by mass spectrometry leaf fingerprinting

Galaverna, Renan; Sampaio, Paulo de Tarso Barbosa; Barata, Lauro Euclides Soares; Eberlin, Marcos N.; Fidelis, Carlos H.V.

doi:10.1039/c4ay02598a

Cited by 17 publications

(15 citation statements)

References 39 publications

(25 reference statements)

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“…In negative mode, this compound presented 3 pseudomolecular ions [M+Cl] − at m/z 487 and 489, [M+COOH] − at m/z 497. The compound showed characteristic MS 2 fragments of (epi)catechin (Galaverna, Sampaio, Barata, Eberlin, & Fidelis, ; Kang, Price, Ashton, Tapsell, & Johnson, ), so it was tentatively identified as (+)‐(epi)catechin‐ O ‐hexoside.…”

Section: Resultsmentioning

confidence: 99%

UHPLC/MS Identifying Potent α‐glucosidase Inhibitors of Grape Pomace via Enzyme Immobilized Method

Zhu

Sun

Yang

et al. 2018

Journal of Food Science

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α -Glucosidases have been a major target in controlling and managing postprandial blood glucose and therefore diabetes treatment. This study aims to further identify and purify active compounds from the most active ethyl acetate fraction collected previously in Tinta Cão grape pomace extract (TCEE) using a newly developed and highly effective immobilization method, including obtaining compounds previously shown to inhibit the enzyme. Purification used crosslinked chitosan beads with α-glucosidases bound to polymer, which acted as immobilized enzyme vehicle to collect inhibitors. Compounds absorbed into the beads were eluded using methanol, where collected fraction was subjected to UHPLC-MS analysis to identify active compounds. Results presented 5 major compounds: viniferifuran (amurensin H), p-coumaroyl-6-O-D-glucopyranoside, p-coumaroyl-6-O-hexoside, (epi)catechin-hexoside, 10-carboxyl-pyranopeonidin 3-O-(6″-O-p-coumaroyl)-glucoside. These findings indicated the particular molecules can be utilized as potent α-glucosidases inhibitors, and may be further tested for postprandial glucose control.

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Section: Resultsmentioning

confidence: 99%

UHPLC/MS Identifying Potent α‐glucosidase Inhibitors of Grape Pomace via Enzyme Immobilized Method

Zhu

Sun

Yang

et al. 2018

Journal of Food Science

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“…To evaluate the culture establishment procedure on samples of S. pulcherrimum twenty-seven compounds, listed in Table S8, were used as markers. Of this total, fourteen were identified as tannins through data compared with those described in the literature [63,67] or with spectra libraries (GNPS, METLIN, and HMDB). After comparing the samples in natura with the samples collected at each step of the in vitro culture establishment procedure of S. pulcherrimum on the LC-MS directed metabolic study, significant changes were observed.…”

Section: Stryphnodendron Pulcherrimum (Willd) Hochrmentioning

confidence: 99%

Secure and Sustainable Sourcing of Plant Tissues for the Exhaustive Exploration of Their Chemodiversity

et al. 2020

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The main challenge of plant chemical diversity exploration is how to develop tools to study exhaustively plant tissues. Their sustainable sourcing is a limitation as bioguided strategies and dereplication need quite large amounts of plant material. We examine if alternative solutions could overcome these difficulties by obtaining a secure, sustainable, and scalable source of tissues able to biosynthesize an array of metabolites. As this approach would be as independent of the botanical origin as possible, we chose eight plant species from different families. We applied a four steps culture establishment procedure, monitoring targeted compounds through mass spectrometry-based analytical methods. We also characterized the capacities of leaf explants in culture to produce diverse secondary metabolites. In vitro cultures were successfully established for six species with leaf explants still producing a diversity of compounds after the culture establishment procedure. Furthermore, explants from leaves of axenic plantlets were also analyzed. The detection of marker compounds was confirmed after six days in culture for all tested species. Our results show that the first stage of this approach aiming at easing exploration of plant chemodiversity was completed, and leaf tissues could offer an interesting alternative providing a constant source of natural compounds.

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“…Among the few studies on Pará state samples, A. rosiodora from Emílio Goeldi Museum campus (city of Belém), the flavonoid pinocembrin was the major compound identified in the leaves, using mass spectra [ 9 ]. Analysis of seedling’s leaves from A. rosiodora collected in the Ducke Forest Reserve (Manaus, Amazonas) indicated the benzophenones cotoin and hydroxycotoin as key compounds for these seedlings [ 11 ]. Cotoin is found in high amounts in the A. rosiodora woods [ 24 ], but is not common in leaves.…”

Section: Discussionmentioning

confidence: 99%

“…Due to extreme difficulties in distinguishing specimens based on morphology [ 7 ], it is not known whether the phytochemical variants align with the taxonomic varieties. The majority of the studies focused on the chemical and genetic variability in plants from the Amazon State [ 11 , 12 ]. As a further complication, rosewood is frequently misidentified as other species, such as Aniba parviflora (Meisn.)…”

Section: Introductionmentioning

confidence: 99%

Chemical and Genotypic Variations in Aniba rosiodora from the Brazilian Amazon Forest

et al. 2020

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Aniba rosiodora has been exploited since the end of the nineteenth century for its essential oil, a valuable ingredient in the perfumery industry. This species occurs mainly in Northern South America, and the morphological similarity among different Aniba species often leads to misidentification, which impacts the consistency of products obtained from these plants. Hence, we compared the profiles of volatile organic compounds (essential oils) and non-volatile organic compounds (methanolic extracts) of two populations of A. rosiodora from the RESEX and FLONA conservation units, which are separated by the Tapajós River in Western Pará State. The phytochemical profile indicated a substantial difference between the two populations: samples from RESEX present α-phellandrene (22.8%) and linalool (39.6%) in their essential oil composition, while samples from FLONA contain mainly linalool (83.7%). The comparison between phytochemical profiles and phylogenetic data indicates a clear difference, implying genetic distinction between these populations.

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Differentiation of two morphologically similar Amazonian Aniba species by mass spectrometry leaf fingerprinting

Abstract: Leaves from the morphologically similar Aniba rosaeodora and Aniba parviflora were used to obtain ESI and VL-EASI-MS fingerprinting spectra. The method differentiated rapidly and unequivocally the two species.

Cited by 17 publications

References 39 publications

UHPLC/MS Identifying Potent α‐glucosidase Inhibitors of Grape Pomace via Enzyme Immobilized Method

UHPLC/MS Identifying Potent α‐glucosidase Inhibitors of Grape Pomace via Enzyme Immobilized Method

Secure and Sustainable Sourcing of Plant Tissues for the Exhaustive Exploration of Their Chemodiversity

Chemical and Genotypic Variations in Aniba rosiodora from the Brazilian Amazon Forest

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