Differentiation of type 2 diabetes mellitus with different complications by proteomic analysis of plasma low abundance proteins

Yeh, Shu-Hui; Chang, Wenxiu; Chuang, Hai‐Hua; Huang, Hui-Cheng; Liu, Rue‐Tsuan; Yang, Kuender D.

doi:10.1186/s40200-016-0246-6

Cited by 16 publications

(14 citation statements)

References 24 publications

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“…7 The stomach has emerged as a significant target for therapeutic interventions related to obesity. 11 In our study, L1CAM gene expression as significantly higher in the gut mucosa of OB and OD group subjects compared with the control group. It places a spotlight on the gut-brain axis as a control system regulating energy metabolism.…”

Section: Discussionsupporting

confidence: 49%

Key pathway and gene alterations in the gastric mucosa associated with obesity and obesity‐related diabetes

Wen

Qian

Zhang

et al. 2018

J of Cellular Biochemistry

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Background and Objective The stomach plays an important role in obesity and obesity‐related diabetes; yet, little is known about key pathways in the gastric mucosa associated with obesity and diabetes. Methods We performed gene microarray and real time‐polymerase chain reaction (RT‐PCR) on gut mucosa samples from control subjects (CON), patients with simple obesity (OB), and patients with obesity and comorbid diabetes (OD) (n = 3 per group). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to predict the functional significance of differentially expressed genes. Results In total, 262 genes were upregulated and 265 genes were downregulated in the OB group whereas 1756 genes were upregulated and 1053 genes were downregulated in the OD group compared with the CON group. Of these, 23 were co‐regulated in both comparisons. Seven differentially expressed genes were validated by RT‐PCR (NRIP3, L1CAM, TPO, P2RY1, OR8A1, ADAMTS19, and ASIC3). A functional analysis revealed that genes differentially expressed between the OB or OD and CON groups played crucial roles in metabolic, T cell, and G‐protein coupled receptor biological processes, and primarily participated in the PI3K‐Akt and AGE‐RAGE signaling pathways. Conclusions Obesity and obesity‐related diabetes are associated with important gene expression and pathway alterations in the stomach.

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Section: Discussionsupporting

confidence: 49%

Key pathway and gene alterations in the gastric mucosa associated with obesity and obesity‐related diabetes

Wen

Qian

Zhang

et al. 2018

J of Cellular Biochemistry

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“…Our experimental data as well as and other recent data in the scientific literature [15][16][17][18][19][20] could stimulate development of new high sensitive diagnostic/prognostic proteomic biomarkers -included in the test systems and algorithms for early prediction, prognosis and treatment of T2DM and its main complications as retinopathy, nephropathy, neuropathy, angiopathy, etc. Using proteomic biotechnology for assessment of levels high sensitive proteomic biomarkers in the blood plasma of diabetic patients [16] as well as experimental animal models of disease [17], the metabolic pathway, called proteomic, could be elucidated in T2DM [18]. Thus, specific proteomic biomarkers/indicators could support not only diagnosis of diabetes, but also differential diagnosis of T2DM complications [18][19][20].…”

Section: Proteomic Assays Of Blood Plasma In Patients With Diabetes Mmentioning

confidence: 75%

“…Using proteomic biotechnology for assessment of levels high sensitive proteomic biomarkers in the blood plasma of diabetic patients [16] as well as experimental animal models of disease [17], the metabolic pathway, called proteomic, could be elucidated in T2DM [18]. Thus, specific proteomic biomarkers/indicators could support not only diagnosis of diabetes, but also differential diagnosis of T2DM complications [18][19][20]. In this sense, further utilizing of T2DM proteomic biomarkers in clinical practice could help for successful diagnosis, treatment and stratification of diabetic patients.…”

Section: Proteomic Assays Of Blood Plasma In Patients With Diabetes Mmentioning

confidence: 99%

Protein spectrum of blood plasma in patients with diabetes mellitus type II

Ионова

Ланцова

Tkach

et al. 2018

JCB

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BACKGROUND: The cumulating long-term changes in stable macromolecules, including non-enzymatic glycosylation of proteins and oxidative stress, are main fundamental reasons for development of late complications in patients with diabetes mellitus type II. OBJECTIVE: To evaluate some high-sensitive and specific proteins as early diagnostic tool and prognostic biomarkers of late complications of diabetes mellitus type 2 (T2DM). METHODS: Proteomic studies of blood plasma in patients with T2DM were performed through one-and two-dimensional electrophoresis of proteins in polyacrylamide gel, in the presence of sodium dodecyl sulfate-according to Laemmli (SDS PAGE). RESULTS: The spectrum of 70 proteins was proposed as tools in clinical/laboratory diagnostics and as independent prognostic biomarkers-predicting early development of disease complications. CONCLUSIONS: The identified proteomic biomarkers in blood plasma of patients with T2DM could serve as drug targets for early prevention and successful treatment of disease complications.

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“… 60 HCK belongs to the Src family kinase involved in regulating endothelial cell proliferation, migration, and angiogenesis, 61 and it is differentially expressed between DM and normal subjects, as well as between healthy controls and patients with diabetic peripheral neuropathy. 62 , 63 However, its role in DR progression has not yet been elucidated. HLA-DRB1, for its part, is a vision-related gene that is differentially expressed in proliferative DR lesions, 64 while its polymorphisms are known risk factors for DM and its complications.…”

Section: Discussionmentioning

confidence: 99%