Differentiation of virulent and non‐virulent strains of Newcastle disease virus within 24 hours by polymerase chain reaction

Kant, A.; Koch, G.; Roozelaar, D J van; Balk, F.; Huurne, Agnes ter

doi:10.1080/03079459708419257

Cited by 96 publications

(102 citation statements)

References 18 publications

Supporting

Mentioning

100

Contrasting

Unclassified

Order By: Relevance

“…However, in this research RT-PCR was performed to detect NDV directly from chicken organ samples. According to Kant et al (1997) found direct RT-PCR detection from organ samples containing virulent NDV to be possible, but failed to detect NDV in some tissue samples containing nonvirulent NDV due to the assay sensitivity. Another report by Gohm et al (2000) states that rapid NDV detection is achieved by employing RT-PCR directly on samples from affected birds without prior virus isolation.…”

Section: Resultsmentioning

confidence: 99%

Newcastle Disease Virus Detection from Chicken Organ Samples Using Reverse Transcriptase Polymerase Chain Reaction

Shanmuganathan¹,

Anggoro²,

Wibowo³

2017

Jurnal Sain Veteriner

View full text Add to dashboard Cite

Newcastle disease (ND) is a systemic, viral respiratory disease that is acute and easily transmitted which affects various types of poultry, especially chickens. Diagnosis of ND which generally involves virus isolation and subsequent identification with serological assays has limitations that needs more time. This research was aimed to detect Newcastle Disease virus (NDV) in chickens suspected with ND using the Reverse TranscriptasePolymerase Chain Reaction (RT-PCR) technique. Nine chicken organ samples such as lien, trachea, and lungs were collected from chicken farms diagnosed with ND. The organ samples were processed and the targeted viral RNA was extracted using the RNA extraction kit. Genome amplification was performed with RT-PCR using specific primers to target the F gene. Amplification results produced an amplicon product of 565 base pairs (bp). PCR product samples were then visualised using agar gel electrophoresis and viewed using the unified gel documentation system. Amplification results show nine samples positive for the DNA bands corresponding to the targeted band of the NDV F gene fragment. The results of this research confirm that the RT-PCR method is applicable for NDV detection from chicken organ samples.Keywords: Newcastle Disease virus, organ sample, amplification. AbstrakNewcastle Disease (ND) adalah suatu penyakit pernafasan dan sistemik, yang bersifat akut dan mudah menular, yang disebabkan oleh virus dan menyerang berbagai jenis unggas, terutama ayam. Diagnosis penyakit ND yang pada umumnya dilakukan dengan isolasi virus dan identifikasi serologis memiliki keterbatasan, antara lain diperlukan waktu yang lebih lama. Penelitian ini bertujuan mendeteksi virus Newcastle Disease (NDV) pada ayam yang dicurigai dengan penyakit ND dengan menggunakan teknik Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Sembilan sampel organ ayam yaitu: lien, trakea, dan paru dikoleksi dari peternakan ayam yang didiagnosis penyakit ND. Sampel organ tersebut diproses dan diekstraksi RNA virus target dengan kit ekstraksi RNA. Amplifikasi genom virus dilakukan dengan RT-PCR menggunakan primer spesifik pada target gen F. Hasil amplifikasi menghasilkan produk amplikon sebesar 565 base pairs (bp). Sampel produk PCR kemudian divisualisasikan menggunakan gel elektroforesis dan diamati menggunakan unified gel documentation system. Hasil amplifikasi menunjukkan sembilan sampel positif teramati pita DNA sesuai target fragmen gen F virus ND. Hasil penelitian ini mengkonfirmasi bahwa metode RT-PCR dapat digunakan untuk mendeteksi virus ND dari sampel organ ayam.Kata Kunci: virus Newcastle Disease, sampel organ, amplifikasi.

show abstract

Section: Resultsmentioning

confidence: 99%

Newcastle Disease Virus Detection from Chicken Organ Samples Using Reverse Transcriptase Polymerase Chain Reaction

Shanmuganathan¹,

Anggoro²,

Wibowo³

2017

Jurnal Sain Veteriner

View full text Add to dashboard Cite

show abstract

“…The presence of virulent F protein in the NDV positive isolates in the HI test was evaluated by the reverse transcription polymerase chain reaction method (RT-PCR) using Kant et al primers (16). Two different 1-step RT-PCR reactions, targeting the F gene, were performed using primer pairs A+B and A+C as general primers and virulent pathotype-specific primers, respectively (16) ( Table 1).…”

Section: Molecular Pathogenicity Assessmentmentioning

confidence: 99%

Pathotypic characterization of the Newcastle disease virus isolated from commercial poultry in northwest Iran

Ahmadi¹,

Pourbakhsh²,

Ahmadi³

et al. 2014

Turk J Vet Anim Sci

View full text Add to dashboard Cite

Despite vaccination, outbreaks of Newcastle disease in commercial poultry flocks in northwest Iran have been regularly reported in recent years. This research was proposed in order to characterize, through a molecular approach, the genetic nature of strains of Newcastle disease virus isolated from commercial poultry flocks in northwest Iran. Ten Newcastle disease virus isolates were obtained from various districts of northwest Iran in 2010. The isolates were screened with F gene-targeting reverse transcription polymerase chain reaction (RT-PCR). The amplified products of virulent strains were sequenced. The deduced amino acid sequence of the fusion protein cleavage site indicated the presence of motif 112 R-R-Q-R-R↓F 117 , typical for velogenic strains of NDV. These results confirmed that virulent NDVs are circulating in the region, causing economic losses within the poultry industry.

show abstract

“…The frequency of detection of field virus is likely to be dependent, in part, on whether birds have previously received APV vaccine; replication of field virus is likely to be to a lower level in vaccinated birds, because of immunity induced by the vaccine, thereby reducing the window of opportunity for detection. Kant et al (1997) have devised a pathotype-specifi c RT-PCR for NDV, the specificity being based on sequences of that part of the F protein gene that encodes the peptide that connects the F2 and F1 subunits, and at which cleavage occurs during the maturation of the F protein. It is the sequence of that peptide that determines in which tissues the F protein will be cleaved; pathogenicity is often a direct consequence of this.…”

Section: Avian Pneumovirusmentioning

confidence: 99%

Innovation and discovery: The application of nucleic acid-based technology to avian virus detection and characterization

Cavanagh¹

2001

Avian Pathology

View full text Add to dashboard Cite

Polymerase chain reaction (PCR)-based approaches to the detection, differentiation and characterization of avian pathogens continue to be developed and refined. The PCRs, or reverse transcriptasePCRs, may be general, designed to detect all or most variants of a pathogen, or to be serotype, genotype or pathotype specific. Progress is being made with respect to making nucleic acid approaches more suitable for use in diagnostic laboratories. Robotic workstations are now available for extraction of nucleic acid from many samples in a short time, for routine diagnosis. Following general PCR, the DNA products are commonly analyzed by restriction endonuclease mapping (restriction fragment length polymorphism), using a small number of restriction endonucleases, based on a large body of sequence data. Increasingly, however, nucleotide sequencing is being used to analyze the DNA product, in part due to the expanding use of non-radioactive sequencing methods that are safe and enable high throughout. In this review, I highlight some recent developments with many avian viruses: Newcastle disease virus; circoviruses in canary and pigeon; infectious bursal disease virus (Gumboro disease virus); avian adenoviruses, including Angara disease/infectious hydropericardium virus, haemorrhagic enteritis virus of turkeys, and egg drop syndrome virus; avian herpesviruses, including infectious laryngotracheitis virus, duck plague virus, psittacine herpesvirus (Pacheco's parrot disease virus), Marek's disease virus and herpesvirus of turkeys; avian leukosis virus (associated with lymphoid leukosis or myeloid leukosis, and egg transmission); avian pneumoviruses (turkey rhinotracheitis virus); avian coronaviruses, including infectious bronchitis virus, turkey coronavirus and pheasant coronavirus; astrovirus, in the context of poult enteritis and mortality syndrome, and avian nephritis virus; and avian encephalomyelitis virus, a picornavirus related to hepatitis A virus.

show abstract

Differentiation of virulent and non‐virulent strains of Newcastle disease virus within 24 hours by polymerase chain reaction

Cited by 96 publications

References 18 publications

Newcastle Disease Virus Detection from Chicken Organ Samples Using Reverse Transcriptase Polymerase Chain Reaction

Newcastle Disease Virus Detection from Chicken Organ Samples Using Reverse Transcriptase Polymerase Chain Reaction

Pathotypic characterization of the Newcastle disease virus isolated from commercial poultry in northwest Iran

Innovation and discovery: The application of nucleic acid-based technology to avian virus detection and characterization

Contact Info

Product

Resources

About