Differentiation potential of germ line stem cells derived from the postnatal mouse ovary

Pacchiarotti, Jason; Maki, Chad B.; Ramos, Thomas; Marh, Joel; Howerton, Kyle; Wong, Jadelind; Pham, Jane; Anorve, Sandra; Chow, Yung-Chiong; Izadyar, F.

doi:10.1016/j.diff.2010.01.001

Cited by 154 publications

(192 citation statements)

References 29 publications

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“…The isolation of fresh FGSCs in mouse and in human ovaries relies on the binding to live cells of an antibody raised against the C-terminus of Ddx4, or Vasa homologue (Zou et al 2009, Pacchiarotti et al 2010. Although the presence of plasma membrane Ddx4 remains questionable until a clear image of the plasmalemma distribution of the protein is obtained and an attempt to isolate FGSCs from Ddx4 null mice is performed, immunopurification of FGSCs has been reproduced independently in many laboratories worldwide.…”

Section: Fgscs Could Originate From Undifferentiated Pgcs or A Subpopmentioning

confidence: 99%

Seeking the origin of female germline stem cells in the mammalian ovary

Felici

Barrios

2013

Reproduction

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The function of female germline stem cells (FGSCs, also called oogonial stem cells) in the adult mammalian ovary is currently debated in the scientific community. As the evidence to support or discard the possible crucial role of this new class of germ cells in mammals has been extensively discussed, in this review, we wonder which could be their origin. We will assume that FGSCs are present in the post-natal ovaries and speculate as to what origin and characteristics such cells could have. We believe that the definition of these features might shed light on future experimental approaches that could clarify the ongoing debate.Reproduction (2013) 146 R125-R130

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Section: Fgscs Could Originate From Undifferentiated Pgcs or A Subpopmentioning

confidence: 99%

Seeking the origin of female germline stem cells in the mammalian ovary

Felici

Barrios

2013

Reproduction

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“…multifluorescent tracing | oogonial stem cells | female germline stem cells I t has been generally accepted for more than half a century that in most mammalian species oocytes cannot renew themselves in postnatal or adult life, and that the number of oocytes is already fixed in fetal or neonatal ovaries (1,2). This assumption, however, has been challenged over the past decade (3)(4)(5)(6)(7).…”

mentioning

confidence: 99%

“…As various laboratories in the field have great interest in this topic but have been unable to find functional roles for the isolated cells (6,12), the key question is whether the cells purified using the DDX4 antibody are meiotically competent and can differentiate into functional oocytes. It is also of great importance to validate the conclusions drawn by Zou et al (5) and White et al (4) with an experimental approach other than an antibody-based cell purification.…”

mentioning

confidence: 99%

Experimental evidence showing that no mitotically active female germline progenitors exist in postnatal mouse ovaries

Zhang

Zheng

Shen

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

194

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It has been generally accepted for more than half a century that, in most mammalian species, oocytes cannot renew themselves in postnatal or adult life, and that the number of oocytes is already fixed in fetal or neonatal ovaries. This assumption, however, has been challenged over the past decade. In this study, we have taken an endogenous genetic approach to this question and generated a multiple fluorescent Rosa26 rbw/+ ;Ddx4-Cre germline reporter mouse model for in vivo and in vitro tracing of the development of female germline cell lineage. Through live cell imaging and de novo folliculogenesis experiments, we show that the Ddx4-expressing cells from postnatal mouse ovaries did not enter mitosis, nor did they contribute to oocytes during de novo folliculogenesis. Our results provide evidence that supports the traditional view that no postnatal follicular renewal occurs in mammals, and no mitotically active Ddx4-expressing female germline progenitors exist in postnatal mouse ovaries.

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“…Several studies have pointed toward the presence of mitotically-active germline stem cells in the mammalian ovary (Johnson et al, 2004;Pacchiarotti et al, 2010;Parte et al, 2011;Zou et al, 2009). Many groups have conducted experiments which have isolated stem cells capable of sustaining oocyte and follicle production in vitro.…”

Section: The Significance Of Human Agingmentioning

confidence: 99%