Differentiation potential of neural stem cells derived from fetal sheep

Abstract

Neural stem cells (NSCs) are multipotent stem cells that can differentiate into many cell types in vitro. In this study, we isolated and established an NSC line from fetal Ovis aries. Based on the results of immunofluorescence staining, NSCs expressed Nestin, Pax6 and MAP2. Moreover, a reverse transcription–polymerase chain reaction assay was used to biologically characterize the cell line. NSCs were induced to differentiate into neurogenic cells in vitro. They expressed MAP2, glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP). In this study, we successfully isolated and cultivated NSCs from the hippocampal tissue of fetal sheep. NSCs not only displayed a self-renewal capacity but also had the potential to differentiate into neurons and glial cells. This study provided valuable experimental data for NSC transplant research.

Isolation and characterization of neural stem cells from ovine foetal cerebral cortex

“…The PDT was significantly prolonged, cell transparency and viability decreased beginning at passage10 (Fig 3). The 'S' shaped curve obtained shows similarity with the proliferation rate observed by Li et al (2017); Kumar et al (2013) and Agarwal et al(2014).…”

“…In primary cultures, initial adherent colonies were observed within 2 to 3 days (Fig 1A), the cells grew more rapidly and reached 70-80% confluence on 6 to 7 days of culture. The cells were developing into multiple neurites extending from the cell body whose projections intertwined into a network after a week (Fig1B) as illustrated by Li et al (2017). Morphologically similar cells isolated from goat foetal brain were maintained for several passages by Agarwal et al (2014).…”

“…The NSCs isolated from human and mouse CNS have been shown to exhibit all these markers (Osterberg and Roussa 2009). Apart from these markers, NSCs have been reported to express other NSC markers Sox2, Pax6 (Agarwal et al, 2014), GFAP (Kumar et al, 2013), Notch1, MAP (Li et al, 2017). The neural stem cells isolated from embryonic day 98 foetal goat brain transfected with green fluorescent protein gene expressed neuron specific markers (Zheng et al, 2014).…”

“…Kumar et al (2013) reported that the neural stem cells can be isolated and expanded rapidly from buffalo foetal brain in adherent culture and expressed neuronal markers like β tubulin III and GFAP. Li et al (2017) successfully isolated and cultivated NSCs from the hippocampal tissue of foetal sheep and observed the expression of GFAP, s-100β, MAP-2, TUBB3, MBP and MOG genes by RT-PCR.…”

Establishment and evaluation of the goose embryo epithelial (GEE) cell line as a new model for propagation of avian viruses

“…TD = t (lg2 / lgNt—lgN0) (TD and t represent cell doubling time and duration of cell culture, respectively. While, N 0 represent cell number after inoculation and N 1 represent cell number after culturing for t hours [ 15 ]. The Ni was 2000 in each passage and NF was calculated based on number of the harvested cells at 7th day of each passage.…”