Differing Affinities of Gold Nanostars and Nanospheres toward HeLa and HepG2 Cells: Implications for Cancer Therapy

Kashani, Ahmad Sohrabi; Bădilescu, Simona; Piekny, Alisa; Packirisamy, Muthukumaran

doi:10.1021/acsanm.0c00244

Cited by 10 publications

(9 citation statements)

References 46 publications

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“…We applied the same strategy to quantify the uptake of particles in different regions of cells. Considering the size range of each type of particle (measured by DLS), we used ImageJ to count the average number of three different types of NPs in two different cell lines 12 . Student’s t -test was used to compare statistical data, and differences were considered significant at p < 0.05.…”

Section: Methodsmentioning

confidence: 99%

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Using intracellular plasmonics to characterize nanomorphology in human cells

2020

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Determining the characteristics and localization of nanoparticles inside cells is crucial for nanomedicine design for cancer therapy. Hyperspectral imaging is a fast, straightforward, reliable, and accurate method to study the interactions of nanoparticles and intracellular components. With a hyperspectral image, we could collect spectral information consisting of thousands of pixels in a short time. Using hyperspectral images, in this work, we developed a label-free technique to detect nanoparticles in different regions of the cell. This technique is based on plasmonic shifts taking place during the interaction of nanoparticles with the surrounding medium. The unique optical properties of gold nanoparticles, localized surface plasmon resonance bands, are influenced by their microenvironment. The LSPR properties of nanoparticles, hence, could provide information on regions in which nanoparticles are distributed. To examine the potential of this technique for intracellular detection, we used three different types of gold nanoparticles: nanospheres, nanostars and Swarna Bhasma (SB), an Indian Ayurvedic/Sidha medicine, in A549 (human non-small cell lung cancer) and HepG2 (human hepatocellular carcinoma) cells. All three types of particles exhibited broader and longer bands once they were inside cells; however, their plasmonic shifts could change depending on the size and morphology of particles. This technique, along with dark-field images, revealed the uniform distribution of nanospheres in cells and could provide more accurate information on their intracellular microenvironment compared to the other particles. The region-dependent optical responses of nanoparticles in cells highlight the potential application of this technique for subcellular diagnosis when particles with proper size and morphology are chosen to reflect the microenvironment effects properly.

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Section: Methodsmentioning

confidence: 99%

“…Raman spectroscopy (RS) and infrared absorption (IR) have also been used to detect and localize NPs at the subcellular level 12 . The low spatial resolution of the IR technique has limited the application of this technique for intracellular diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Using intracellular plasmonics to characterize nanomorphology in human cells

2020

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“…Different affinities to different subcellular elements may also be an influential factor in the level of impact on mechanobiological properties. Our previous study observed that gold NPs have different affinities to cytoskeletal components. , This morphology-dependent behavior of NPs may influence NBI and, subsequently, their impacts on cellular elasticity. However, a further detailed study is required to investigate the role of NP morphology in their interaction with intracellular proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Zetasizer Nano ZS90 (Malvern Instruments, UK) was utilized to characterize the size of gold NPs. The Brookhaven Zeta Plus electrophoresis instrument was used to characterize the zeta potential of particles under ambient temperature as described previously …”

Section: Methodsmentioning

confidence: 99%

Gold Nano-Bio-Interaction to Modulate Mechanobiological Responses for Cancer Therapy Applications

Kashani

Larocque

Piekny

et al. 2022

ACS Appl. Bio Mater.

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In the present study, we investigate the mechanobiological responses of human lung cancer that may occur through their interactions with two different types of gold nanoparticles: nanostars and nanospheres. Hyperspectral images of nanoparticle-treated cells revealed different spatial distributions of nanoparticles in cells depending on their morphology, with nanospheres being more uniformly distributed in cells than nanostars. Gold nanospheres were also found to be more effective in mechanobiological modulations. They significantly suppressed the migratory ability of cells under different incubation times while lowering the bulk stiffness and adhesion of cells. This in vitro study suggests the potential applications of gold nanoparticles to manage cell migration. Nano-bio-interactions appeared to impact the cytoskeletal organization of cells and consequently alter the mechanical properties of cells, which could influence the cellular functions of cells. According to the results and migratory index model, it is thought that nanoparticle-treated cells experience mechanical changes in their body, which largely reduces their migratory potentials. These findings provide a better understanding of nano-bio-interaction in terms of cell mechanics and highlight the importance of mechanobiological responses in designing gold nanoparticles for cancer therapy.

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“…They also observed that MT networks are damaged and shrunken upon interaction with gold NPs compared to control cells. NPs might have different affinities to different subcellular structures depending on their physicochemical properties [152,153]. Wen et al [154] found that silver NPs tend to bind actin rather than tubules under electrostatic interactions.…”

Section: Impacts Of Nanoparticles On Structural Elements and Morphology Of Cellsmentioning

confidence: 99%

Cancer-Nano-Interaction: From Cellular Uptake to Mechanobiological Responses

Kashani

Packirisamy

2021

IJMS

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With the advancement of nanotechnology, the nano-bio-interaction field has emerged. It is essential to enhance our understanding of nano-bio-interaction in different aspects to design nanomedicines and improve their efficacy for therapeutic and diagnostic applications. Many researchers have extensively studied the toxicological responses of cancer cells to nano-bio-interaction, while their mechanobiological responses have been less investigated. The mechanobiological properties of cells such as elasticity and adhesion play vital roles in cellular functions and cancer progression. Many studies have noticed the impacts of cellular uptake on the structural organization of cells and, in return, the mechanobiology of human cells. Mechanobiological changes induced by the interactions of nanomaterials and cells could alter cellular functions and influence cancer progression. Hence, in addition to biological responses, the possible mechanobiological responses of treated cells should be monitored as a standard methodology to evaluate the efficiency of nanomedicines. Studying the cancer-nano-interaction in the context of cell mechanics takes our knowledge one step closer to designing safe and intelligent nanomedicines. In this review, we briefly discuss how the characteristic properties of nanoparticles influence cellular uptake. Then, we provide insight into the mechanobiological responses that may occur during the nano-bio-interactions, and finally, the important measurement techniques for the mechanobiological characterizations of cells are summarized and compared. Understanding the unknown mechanobiological responses to nano-bio-interaction will help with developing the application of nanoparticles to modulate cell mechanics for controlling cancer progression.

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Differing Affinities of Gold Nanostars and Nanospheres toward HeLa and HepG2 Cells: Implications for Cancer Therapy

Cited by 10 publications

References 46 publications

Using intracellular plasmonics to characterize nanomorphology in human cells

Using intracellular plasmonics to characterize nanomorphology in human cells

Gold Nano-Bio-Interaction to Modulate Mechanobiological Responses for Cancer Therapy Applications

Cancer-Nano-Interaction: From Cellular Uptake to Mechanobiological Responses

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