Diffusion of Lipid Nanovesicles Bound to a Lipid Membrane Is Associated with the Partial-Slip Boundary Condition

Olsén, Erik; Jõemetsa, Silver; González, A.; Joyce, Paul; Zhdanov, Vladimir P.; Midtvedt, Daniel; Höök, Fredrik

doi:10.1021/acs.nanolett.1c02092

Cited by 5 publications

(2 citation statements)

References 32 publications

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“…A lower diffusion coefficient (approximately half of the value found for the Texas Red lipid) was observed for SAv after anchoring it to the biotin lipid (Figure S6b, Supporting Information). Since the diffusion constant is inversely related to the number of lipids in the SLB, [ 39 ] the decrease of the diffusion constant of 1.6 µm 2 s −1 (for the Texas Red lipid in Figure S6a, Supporting Information) to 0.74 µm 2 s −1 (the average diffusion constant value for SAv in the SAv‐anchored SLB in Figure S6b, Supporting Information) confirms that one SAv molecule binds two biotin lipids. The following binding of biotin‐NTA and chelation of Ni 2+ did not change the membrane mobility, as a diffusion coefficient (0.74 µm 2 s −1 ) similar to that of the preceding SAv‐SLB was obtained (Figure S6c, Supporting Information).…”

Section: Resultsmentioning

confidence: 94%

Recruitment of Receptors and Ligands in a Weakly Multivalent System with Omnipresent Signatures of Superselective Binding

Allegri

Huskens

2023

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For instance, virus internalization by cells occurs in specialized regions where the recruitment of receptors takes place. [11] Typically, influenza A viruses infect their host by entering into cells via receptor-mediated endocytosis, [12,13] during which the distribution of corresponding receptors in the cell membrane is rearranged and co-localized, as a direct result of receptor clustering induced by weak and multiple ligand-receptor interactions. Thus, gaining understanding of the mechanism of receptor clustering induced by weakly multivalent interactions is essential in order to understand its effect in virus infection at the molecular level, and also to allow its application as a design rule in artificial self-assembling systems.In most natural biological systems, multivalent interactions are composed of many, but individually weakly binding, interaction pairs, which contributes to the dynamic character of the interactions. [14][15][16][17][18] We can relate this behavior to the fact that the overall affinity (K ov ) can be orders of magnitude higher than the individual, monovalent affinity (K i ). More precisely, each additional interaction pair contributes to the overall affinity by only a small enhancement factor, [1,19] which indicates at the same time that each interaction pair is dynamically equilibrating between its bound and unbound states, the frequency of which is dictated by the intrinsic dissociation rate constant. In contrast, as an opposing example of a strongly binding system, the individual affinity of biotin-streptavidin (SAv) is very high, [20,21] and as a result, assemblies based on multiple biotin-SAv interactions are viewed to be practically irreversible. [22][23][24] As an example of the dynamic nature of weak interactions, it has been reported that the low-density lipoprotein (LDL) receptors in the cell membrane will return to the cell membrane surface after the receptor-mediated endocytosis of a virus particle, where they again cluster in coated pits, [25,26] indicating that the LDL receptors still keep their mobility and are recycled in the recruitment process. This recycling property accounts for the fact that the receptors during clustering are still dynamic at their binding sites, allowing switching between the diffusive and the clustering states.To provide quantitative insight into these molecular processes, it is necessary to build synthetic model systems that Recruitment of receptors at membrane interfaces is essential in biological recognition and uptake processes. The interactions that induce recruitment are typically weak at the level of individual interaction pairs, but are strong and selective at the level of recruited ensembles. Here, a model system is demonstrated, based on the supported lipid bilayer (SLB) that mimics the recruitment process induced by weakly multivalent interactions. The weak (mm range) histidine-nickel-nitrilotriacetate (His 2 -NiNTA) pair is employed owing to its ease of implementation in both synthetic and biological systems. The recruitment of rece...

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Section: Resultsmentioning

confidence: 94%

Recruitment of Receptors and Ligands in a Weakly Multivalent System with Omnipresent Signatures of Superselective Binding

Allegri

Huskens

2023

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“…Curved membrane mimics offer unique platforms for the investigation of complex biophysical interactions . However, their construction on a solid substrate and the subsequent application in SPR-based methods have been very limited to date. ,− We report a straightforward approach in which the membrane construction process can be traced in real time.…”

Section: Resultsmentioning

confidence: 99%

Curved Membrane Mimics for Quantitative Probing of Protein–Membrane Interactions by Surface Plasmon Resonance

Malinick,

Stuart,

Lambert

et al. 2023

ACS Appl. Mater. Interfaces

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A majority of biomimetic membranes used for current biophysical studies rely on planar structures such as supported lipid bilayer (SLB) and self-assembled monolayers (SAMs). While they have facilitated key information collection, the lack of curvature makes these models less effective for the investigation of curvature-dependent protein binding. Here, we report the development and characterization of curved membrane mimics on a solid substrate with tunable curvature and ease in incorporation of cellular membrane components for the study of protein-membrane interactions. The curved membranes were generated with an underlayer lipid membrane composed of DGS-Ni-NTA and POPC lipids on the substrate, followed by the attachment of histidine-tagged cholera toxin (his-CT) as a capture layer. Lipid vesicles containing different compositions of gangliosides, including GA 1 , GM 1 , GT 1b , and GQ 1b , were anchored to the capture layer, providing fixation of the curved membranes with intact structures. Characterization of the curved membrane was accomplished with surface plasmon resonance (SPR), fluorescence recovery after photobleaching (FRAP), and nano-tracking analysis (NTA). Further optimization of the interface was achieved through principal component analysis (PCA) to understand the effect of ganglioside type, percentage, and vesicle dimensions on their interactions with proteins. In addition, Monte Carlo simulations were employed to predict the distribution of the gangliosides and interaction patterns with single point and multipoint binding models. This work provides a reliable approach to generate robust, component-tuning, and curved membranes for investigating protein interactions more pertinently than what a traditional planar membrane offers.

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Diffusion-limited association of nanoparticles in fluid: Beyond the no-slip boundary conditions

Zhdanov

2022

Colloid and Interface Science Communications

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Diffusion of Lipid Nanovesicles Bound to a Lipid Membrane Is Associated with the Partial-Slip Boundary Condition

Cited by 5 publications

References 32 publications

Recruitment of Receptors and Ligands in a Weakly Multivalent System with Omnipresent Signatures of Superselective Binding

Recruitment of Receptors and Ligands in a Weakly Multivalent System with Omnipresent Signatures of Superselective Binding

Curved Membrane Mimics for Quantitative Probing of Protein–Membrane Interactions by Surface Plasmon Resonance

Diffusion-limited association of nanoparticles in fluid: Beyond the no-slip boundary conditions

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