Digested protein isolate from fresh and stored Carioca beans reduced markers of atherosclerosis in oxidized LDL-induced THP-1 macrophages

Alves, Natália Elizabeth Galdino; Vasconcelos, Christiane Mileib; Bassinello, P. Z.; Mejía, Elvira González de; Verediano, Thaísa Agrizzi

doi:10.1016/j.jff.2016.03.027

Cited by 12 publications

(9 citation statements)

References 51 publications

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“…The present study evaluated the protective effect of common bean protein hydrolysate on the vascular endothelium of BALB/c mice fed an atherogenic diet. Common beans and their protein hydrolysate were regarded as source of phenolic compounds and bioactive peptides with antihyperlipidemic, anti-inflammatory and antihypertensive effects [5,6,31,32].…”

Section: Discussionmentioning

confidence: 99%

“…MDA concentration in APH group was lower than in AD group, indicating that treatment could attenuate oxidative stress. It occurs possibly due to the action of bean protein hydrolysate, phytochemicals and bioactive peptides with antioxidant and anti-inflammatory action, which may neutralize free radicals and prevent lipid peroxidation caused by the atherogenic diet [5,6,43,44]. The total antioxidant capacity (TAC) in APH group serum was similar to the AD group.…”

Section: Discussionmentioning

confidence: 99%

“…The proposed regulatory mechanism suggests that ACE inhibition may favor the expression of e-NOS, which in turn stimulates the synthesis and release of nitric oxide in vascular endothelium and contributes to vasodilatory action [7,8]. Other authors also found bioactive peptides inhibiting ACE in legumes and proposed their action in regulatory pathway of atherosclerosis [5,6,51,52], but without in vivo evidence.…”

Section: Discussionmentioning

confidence: 99%

“…The protein hydrolysate (PH) was obtained by a simulated gastrointestinal digestion process according to Alves et al [5] using pepsin and pancreatin [13]. Peptides were previously identified and characterized by Alves et al [6] using size exclusion chromatography and high-performance liquid chromatography-electrospray-ionization-mass spectrometry (HPLCeESIeMS). In the aforementioned study, the bioactive peptide sequences were confirmed using UniProt database from BLASTÒ tool (http://www.blast.ncbi.nlm.…”

Section: Sample Materialsmentioning

confidence: 99%

“…Common bean (Phaseolus vulgaris L.) is a widely consumed legume in Brazil and other countries [4]. Its protein hydrolysate has demonstrated in vitro antihyperlipidemic, anti-inflammatory and antihypertensive properties [5,6], being related to the sequences of antioxidant bioactive peptides and blood pressure regulators. Some of these peptides are able to block the action of angiotensin-converting enzyme (ACE), preventing the conversion of angiotensin I to angiotensin II, a potent vasoconstrictor.…”

Section: Introductionmentioning

confidence: 99%

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Common bean protein hydrolysate modulates lipid metabolism and prevents endothelial dysfunction in BALB/c mice fed an atherogenic diet

Gomes

Lima

Alves

et al. 2020

Nutrition, Metabolism and Cardiovascular Diseases

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Background and aims: Common beans (Phaseolus vulgaris L.) protein hydrolysate is a source of bioactive peptides with known health benefits. The aim of this study was to evaluate the effect of common bean protein hydrolysate on lipid metabolism and endothelial function in male adult BALB/c mice fed an atherogenic diet for nine weeks. Methods and results: Male adult mice were divided into three experimental groups (n Z 12) and fed with normal control diet; atherogenic diet and atherogenic diet added with bean protein hydrolysate (700 mg/kg/day) for nine weeks. Food intake, weight gain, lipid profile, Atherogenic Index of Plasma, inflammation biomarkers and endothelial function were evaluated. APH group presented reduced feed intake, weight gain, lipid profile, tumor necrosis factor-a, angiotensin II (94% and 79%, respectively) and increased endothelial nitric oxide synthase (62%). Conclusions: Protein hydrolysate showed hypocholesterolemic activity preventing inflammation and dysfunction of vascular endothelium, in addition to decreasing oxidative stress, indicating an adjuvant effect on reducing atherogenic risk.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Sample Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Common bean protein hydrolysate modulates lipid metabolism and prevents endothelial dysfunction in BALB/c mice fed an atherogenic diet

Gomes

Lima

Alves

et al. 2020

Nutrition, Metabolism and Cardiovascular Diseases

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Benefits of Carioca Beans ( Phaseolus vulgaris )

Bento¹,

Gomes²,

Bassinello³

et al. 2022

Molecular Mechanisms of Functional Food

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Dry bean proteins: Extraction methods, functionality, and application in products for human consumption

et al. 2021

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Background and objectives Legume proteins, such as those from dried beans, are extracted by methods that can be divided into aqueous or dry fractionation processes. Several techniques have been studied to improve protein extraction in legumes and their functional properties. This article provides relevant information on available extraction methods, their advantages and disadvantages, and the functionality and application of dried bean protein in the manufacture of food products. Findings It is possible to obtain yields of up to 93% in dry bean protein extraction by the aqueous fractionation method, resulting in concentrates with a protein content above 70%. The combination of other treatments (thermal or not), applied before or after extraction, can improve the extraction yield and the functional properties of proteins, respectively. Dry fractionation allows yields of up to 30%, which can be improved with electrostatic separation. Bean proteins have equal or superior performance to soybeans in terms of emulsifying properties, high hydrophobicity, and foaming. Conclusions Dry fractionation is a more sustainable method of protein extraction, as it uses few resources and preserves the native properties of proteins. Aqueous fractionation guarantees a higher protein yield. Dried beans are a raw material with enormous potential for protein extraction and application of its protein in the manufacture of vegetable‐based food products with added value and high nutritional and health benefits, such as vegetable meats, for example, which are part of the trend of this decade. Significance and novelty This review provides insights into the different bean protein extraction methods and their combinations and how the functional properties can be influenced by them, providing the reader with an available resource to select the most appropriate extraction technique(s) for its application in food.

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Digested protein isolate from fresh and stored Carioca beans reduced markers of atherosclerosis in oxidized LDL-induced THP-1 macrophages

Cited by 12 publications

References 51 publications

Common bean protein hydrolysate modulates lipid metabolism and prevents endothelial dysfunction in BALB/c mice fed an atherogenic diet

Common bean protein hydrolysate modulates lipid metabolism and prevents endothelial dysfunction in BALB/c mice fed an atherogenic diet

Benefits of Carioca Beans ( Phaseolus vulgaris )

Dry bean proteins: Extraction methods, functionality, and application in products for human consumption

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