Digestibility of fucosylated glycosaminoglycan from sea cucumber and its effects on digestive enzymes under simulated salivary and gastrointestinal conditions

Zhao, Longyan; Qin, Yujing; Guan, Ruowei; Zheng, Wenqi; Liu, Ji‐Kai; Zhao, Jinhua

doi:10.1016/j.carbpol.2018.01.029

Cited by 43 publications

(42 citation statements)

References 53 publications

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“…In addition, giant grouper larvae fed maize starch or maltose showed significantly higher intestinal lipase specific activity than fish fed cellulose (Lu et al, ). However, in contrast to our findings, intestinal digestive enzyme activities were inhibited under simulated conditions after administration of polysaccharides, including pectic polysaccharides (Espinal‐Ruiz, Parada‐Alfonso, Restrepo‐Sánchez, & Narváez‐Cuenca, ) and fucosylated glycosaminoglycan (Zhao et al, ). The activity of digestive enzymes reflects the ability of fish to utilize different nutrients (Hidalgo, Urea, & Sanz, ).…”

Section: Discussioncontrasting

confidence: 99%

“…However, in contrast to our findings, intestinal digestive enzyme activities were inhibited under simulated conditions after administration of polysaccharides, including pectic polysaccharides (Espinal-Ruiz, Parada-Alfonso, Restrepo-Sánchez, & Narváez-Cuenca, 2014) and fucosylated glycosaminoglycan(Zhao et al, 2018). Several studies have described positive effects of dietary polysaccharides on the activities of fish digestive enzymes.…”

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confidence: 99%

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Effects of dietary Radix Rehmanniae Preparata polysaccharides on the digestive enzymes, morphology, microbial communities and mucosal barrier function of the intestine of Luciobarbus capito

Shan

Feng

et al. 2020

Aquac Res

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In this study, we assessed the effects of Radix Rehmanniae Preparata polysaccharides (RRPP) on the digestive enzymes, morphology, microbial communities and mucosal barrier function of the intestine of Luciobarbus capito. Fish were fed different amounts of RRPP (0, 0.05, 0.1, 0.2 and 0.4%) for 60 days. The results revealed that intestinal amylase and trypsin activities significantly increased after the 0.2% RRPP treatment (p < .05). Increases in intestinal fold height, wall thickness and fold density were observed in Luciobarbus capito fed an RRPP‐supplemented diet, and the best effect was observed in the proximal intestine. Sequencing of the bacterial 16S rRNA V4 region showed that dietary RRPP reduced intestinal microbial community richness and species diversity. Fusobacteria, Proteobacteria, Firmicutes and Verrucomicrobia were the dominant bacterial phyla in the intestine of Luciobarbus capito. Dietary RRPP affected the overall microbial community and increased the abundance of the genus Cetobacterium. Moreover, RRPP significantly upregulated the expression of occludin, zonula occludens (ZO)‐1 and claudin‐c in the intestine (p < .05). In summary, supplementing the diet of Luciobarbus capito with RRPP can improve digestive enzymes activities and intestinal morphology, modulate the microbial composition and enhance intestinal mucosal barrier function.

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Section: Discussioncontrasting

confidence: 99%

contrasting

confidence: 99%

Effects of dietary Radix Rehmanniae Preparata polysaccharides on the digestive enzymes, morphology, microbial communities and mucosal barrier function of the intestine of Luciobarbus capito

Shan

Feng

et al. 2020

Aquac Res

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“…The PPP and PPP-2 containing higher uronic acid also showed higher superoxide radical scavenging activity compared with PPP-1a and PPP-1b which contain no uronic acid, consisting with previous reports [ 61 ]. The effects of sulfate and carboxyl groups may be further confirmed by the desulfated and carboxyl-reduced derivatives of these polysaccharides in the future study, referring to our previous study [ 62 ].…”

Section: Resultssupporting

confidence: 62%

Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus

et al. 2018

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Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study. The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%. Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns. Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc. Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively. PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2. PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.

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“…The molar ratio of sulfate to uronic acid groups in AjFG was 3.59, as calculated from its conductometric titration (Figure S2). These negatively charged groups in the AjFG chain may contribute to its pharmacological activities [22].…”

Section: Resultsmentioning

confidence: 99%

Precise Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan from Apostichopus japonicus: Analysis of Its Depolymerized Fragments

et al. 2019

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Apostichopus japonicus is one of the most economically important species in sea cucumber aquaculture in China. Fucosylated glycosaminoglycan from A. japonicus (AjFG) has shown multiple pharmacological activities. However, results from studies on the structure of AjFG are still controversial. In this study, the deaminative depolymerization method that is glycosidic bond-selective was used to prepare the depolymerized products from AjFG (dAjFG), and then a series of purified oligosaccharide fragments such as tri-, hexa-, nona-, and dodecasaccharides were obtained from dAjFG by gel permeation chromatography. The 1D/2D NMR and ESI-MS spectrometry analyses showed that these oligosaccharides had the structural formula of l-FucS-α1,3-d-GlcA-β1,3-{d-GalNAc4S6S-β1,4-[l-FucS-α1,3-]d-GlcA-β1,3-}n-d-anTal-diol4S6S (n = 0, 1, 2, 3; FucS represents Fuc2S4S, Fuc3S4S, or Fuc4S). Thus, the unambiguous structure of native AjFG can be rationally deduced: it had the backbone of {-4-d-GlcA-β1,3-d-GalNAc4S6S-β1-}n, which is similar to chondroitin sulfate E, and each d-GlcA residue in the backbone was branched with a l-FucS monosaccharide at O-3. Bioactivity assays confirmed that dAjFG and nonasaccharides and dodecasaccharides from AjFG had potent anticoagulant activity by intrinsic FXase inhibition while avoiding side effects such as FXII activation and platelet aggregation.

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Digestibility of fucosylated glycosaminoglycan from sea cucumber and its effects on digestive enzymes under simulated salivary and gastrointestinal conditions

Cited by 43 publications

References 53 publications

Effects of dietary Radix Rehmanniae Preparata polysaccharides on the digestive enzymes, morphology, microbial communities and mucosal barrier function of the intestine of Luciobarbus capito

Effects of dietary Radix Rehmanniae Preparata polysaccharides on the digestive enzymes, morphology, microbial communities and mucosal barrier function of the intestine of Luciobarbus capito

Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus

Precise Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan from Apostichopus japonicus: Analysis of Its Depolymerized Fragments

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