Digestion of biological materials for mineral analyses using a combination of wet and dry ashing

Selected Mineral Intakes of Adult African-Americans in the Washington, DC Area

“…Details of the procedure have been published (Hill et al, 1986). In brief, a combination of dry and wet ashing was employed.…”

Low dietary iron restores functional capabilities of both exocrine and endocrine pancreas in copper-deficient rats

“…Liver, heart and pancreas were immediately removed, weighed, and stored at −70°C. Liver portions were taken for analysis of copper and iron concentration [11]. Blood was collected for hematocrit measurement and measurements of plasma insulin.…”

“…This insulin RIA kit utilizes an antibody specific for rat insulin. Other aliquots of pancreatic homogenates were used to measure copper and iron concentration [11] and lipase and amylase activities. Aliquots designated to be used for exocrine pancreatic enzyme activities were diluted with PBS containing 0.1% Triton X-100.…”

Garlic oil extract ameliorates the severity of copper deficiency.

“…They were then decapitated and blood was collected for the measurements of hematocrit, triglycérides, glucose and cholesterol by the automated enzymatic procedure of the centrifichem. Livers were removed for quantitative meas urements of Cu and iron [21] and total lipid [22]. Another liver aliquot was used for the enzymatic analysis of glucose-6-phosphate dehydrogenase (G6PDH) (EC 1.1.1.49) and malic enzyme (ME) (EC 1.1.1.40) [23].…”