Digestion of δ-endotoxin by gut proteases may explain reduced sensitivity of advanced instar larvae of Spodoptera littoralis to CryIC

Keller, Menachem; Sneh, B.; Strizhov, N.; Prudovsky, Evgenya; Regev, Avital; Koncz, Csaba; Schell, Jeff; Zilberstein, Aviah

doi:10.1016/0965-1748(95)00102-6

Cited by 139 publications

(97 citation statements)

References 24 publications

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“…Segundo os autores, larvas nos primeiros ínstares são mais sensíveis às toxinas Bt em razão das alterações na atividade específica de preoteases no suco intestinal. Keller et al (1996) demonstraram que o suco intestinal dos últimos ínstares de S. littoralis tem alta atividade proteolílica, capaz de degradar completamente a proteína Cry 1C. Hardke et al (2011) também observaram alta sobrevivência larval de S. frugiperda -cerca de 83% -, ao se alimentar do híbrido DKC 67-03 YGCB.…”

Section: Resultsunclassified

Respostas da lagarta‑do‑cartucho a milho geneticamente modificado expressando a toxina Cry 1A(b)

Mendes

Boregas

Lopes

et al. 2011

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar parâmetros biológicos da lagarta-do-cartucho do milho (Spodoptera frugiperda), alimentada com híbridos de milho Bt, que expressam a toxina Cry 1A(b), e com seus respectivos isogênicos não Bt. Os experimentos foram realizados no laboratório da Embrapa Milho e Sorgo, em Sete Lagoas, MG. Os parâmetros avaliados foram: sobrevivência de larvas após 48 horas, sobrevivência da fase larval e pré-imaginal, biomassa de larvas aos 14 dias de idade, biomassa de pupas, período de desenvolvimento larval, e não preferência alimentar de larvas do primeiro ínstar. Larvas de S. frugiperda apresentam menor sobrevivência nas primeiras 48 horas de alimentação e durante toda a fase larval, na maioria dos híbridos de milho Bt, em comparação ao milho não Bt. A biomassa de larvas e pupas foi sempre menor no milho Bt, e o período larval e o pré-imaginal, maior. Houve interação entre a toxina Cry 1A(b) e a base genética dos híbridos transgênicos, quanto à sobrevivência e à biomassa larval. Larvas recém-eclodidas de S. frugiperda apresentam preferência pela alimentação em milho não Bt.Termos para indexação: Spodoptera frugiperda, manejo de resistência de insetos, manejo integrado de pragas, resistência de plantas, transgenia. Fall armyworm responses to genetically modified maize expressing the toxin Cry 1A(b)Abstract -The objective of this work was to evaluate biological parameters of maize fall armyworm (Spodoptera frugiperda) fed with Bt maize hybrids expressing the Cry 1A(b) toxin, and with its isogenic non-Bt hybrids. The experiments were carried out in the laboratory of Embrapa Milho e Sorgo, Sete Lagoas, MG, Brazil. The parameters evaluated were: survival of larvae after 48 hours, survival of larval and preimaginal phases, larvae biomass at 14 days of age, pupae biomass, larval development period, and feeding non-preference of first instar larvae. Larvae of S. frugiperda show less resistance in the first 48 hours of feeding and during the entire larval phase in most Bt maize hybrids, in comparison to non-Bt maize. The biomass of larvae and pupae developed in Bt maize was always smaller, and the larval and preimaginal period were longer. There was interaction between the Cry 1A(b) toxin and the genetic base of the transgenic hybrids regarding survival and larval biomass. Newly hatched S. frugiperda larvae show preference for feeding on non-Bt maize.

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Section: Resultsunclassified

Respostas da lagarta‑do‑cartucho a milho geneticamente modificado expressando a toxina Cry 1A(b)

Mendes

Boregas

Lopes

et al. 2011

Pesq. agropec. bras.

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“…The mechanisms of resistance to B. sphaericus are not yet defined (46,47), but more than one mechanism seems to be involved (48). Resistance to B. thuringiensis has resulted from reduced binding of the toxin to the brush border in the lumen of the insect gut (49,50) or by enhanced digestion of toxin by gut proteases (51). The six different toxin types in the B. thuringiensis israelensis strain used for vector control were expected to retard or prevent development of a comprehensive resistance mechanism; however, multitoxin resistance to B. T. israelensis has already appeared (52,53).…”

Section: Resistance To Growth Regulators Ivermectins and Other Micrmentioning

confidence: 99%

Insecticide Resistance and Vector Control

Brogdon

McAllister²

1999

Journal of Agromedicine

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“…Starved larvae may also eat more recombinant yeast cells bearing toxins thus enhancing larval demise [13] . Toxicity of Cry1C against advanced larval stages (third and fifth instars) of Spodoptera littoralis is, indeed, reduced due to high levels of protease activity in the gut which rapidly degrades the ingested toxin [19] . The study on larvicidal effect of TMOF-Bti on Ae.…”

Section: Discussionmentioning

confidence: 99%

“…aegypti larvae outside laboratory. For all concentrations of (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) mg), 100% larval mortality occurred at the end of second week exposure. At the end of the third week larval mortality indicated 95%-100% with 6% for the control.…”

Section: Methodsmentioning

confidence: 99%

Residual effects of TMOF–Bti formulations against 1st instar Aedes aegypti Linnaeus larvae outside laboratory

Saiful

Lau

Sulaiman

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

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Digestion of δ-endotoxin by gut proteases may explain reduced sensitivity of advanced instar larvae of Spodoptera littoralis to CryIC

Cited by 139 publications

References 24 publications

Respostas da lagarta‑do‑cartucho a milho geneticamente modificado expressando a toxina Cry 1A(b)

Respostas da lagarta‑do‑cartucho a milho geneticamente modificado expressando a toxina Cry 1A(b)

Insecticide Resistance and Vector Control

Residual effects of TMOF–Bti formulations against 1st instar Aedes aegypti Linnaeus larvae outside laboratory

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