Residual effects of TMOF–Bti formulations against 1st instar Aedes aegypti Linnaeus larvae outside laboratory

Saiful, A. A.; Lau, Kei May; Sulaiman, Sallehudin; Hidayatulfathi, O

doi:10.1016/s2221-1691(12)60031-8

Cited by 8 publications

(7 citation statements)

References 8 publications

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“…Bacillus thuringiensis israelensis (Bti) is a Gram-positive bacterium that is used as a larvicide to cause death to Aedes larvae. Delta endotoxin from the Bti is ingested by larvae and binds to the cells of the epithelium in the mid gut, causing disruption to the osmotic balance within the mid gut's epithelial cells by forming pores in the cell membrane and lysing the cells [10].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Bacillus thuringiensis Treatment on Aedes Population Using Different Applications at High-Rise Buildings

Zaki

Dom

2020

TropicalMed

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Bacillus thuringiensis israelensis (Bti) is an effective biological insecticide for killing mosquito larvae. However, choosing the suitable application method for larviciding is critical in increasing its effectiveness. Therefore, this study aimed to determine the effectiveness of Bti (VectoBac®) WG using various applications at high-rise buildings. Three different applications of Bti treatment were applied at three high-rise buildings in Bandar Saujana Putra. The ULV machine is used for Pangsapuri Impian, a mist blower for Pangsapuri Seri Saujana and a pressured sprayer for BSP 21. BSP Skypark does not undergo treatment and acts as a control. The efficacy of Bti treatment was measured by analyzing the ovitrap surveillance data collected (POI and MLT) for pre and post-treatment. Post-treatment ovitrap surveillance indicates that the Aedes sp. mosquito density was lower than the density at the time of pre-treatment surveillance. Overall, the Aedes albopictus species in both an indoor and outdoor environment setting had shown a reduction. The highest Aedes sp. density reduction is seen through the use of mist blowers in outdoor settings for Aedes albopictus, (%POI reduction = 87.4%; %MLT reduction = 93.8%). The mist blower yielded results that is significantly higher compared to other larviciding applications; the order from greatest to the least was mist blower > pressured sprayer > ULV. It can be concluded that each application produces different degrees of effectiveness in reducing the Aedes sp. density in different environmental settings.

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Section: Introductionmentioning

confidence: 99%

Efficacy of Bacillus thuringiensis Treatment on Aedes Population Using Different Applications at High-Rise Buildings

Zaki

Dom

2020

TropicalMed

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“…Previous studies reported that TMOF enhanced the effectiveness of other biological insecticides against Ae. agypti (Borovsky et al 2010;Borovsky et al 2011;Lau et al 2011;Saiful et al 2012 andKamareddine et al 2013). Combination of TMOF and δ-endotoxin Cry11Aa of Bti show synergistic effect and is highly toxic on Ae.…”

Section: Discussionmentioning

confidence: 99%

Synergistic activity of Bacillus thuringiensis δ‐endotoxin and TMOF against Culex pipiens and Spodoptera littoralis larvae

Mohammed

Diab

Khalil

2017

Entomological Research

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Trypsin Modulating Oostatic Factor (TMOF) is a decapeptide hormone that inhibits the biosynthesis of digestive enzymes in the mosquito midgut. The hormone inhibits food digestion and ultimately leads to starvation and death. It has been used as a biological insecticide to control mosquitoes. In an attempt to increase the insecticidal activity of TMOF, a combination of CryIC (δ‐endotoxin from Bacillus thuringiensis) and TMOF was determined. Eight recombinant proteins fused with GST (glutathione‐S‐transferase) were expressed in Escherichia coli cells. Their insecticidal activities were determined against Culex pipiens and Spodoptera littoralis larvae. Purified GST‐TMOF and its analogue GST‐YDPAS exhibited a moderate toxicity on C. pipiens larvae with LC50 of 145.9 and 339.9 μg/mL, respectively. Unexpectedly, no mortality was observed in first instar larvae of S. littoralis. Puirified GST‐TMOF and GST‐YDPAS together with Bt toxin showed a synergistic toxic effect on both Culex and Spodoptera larvae. In the presence of 100 μg/mL GST‐TMOF and GST‐YDPAS, the median lethal concentration of entomocidus on culex larvae decreased from 52.1 to 16.7 and 31.9 μg/mL, respectively. Likewise, GST‐TMOF and GST‐YDPAS incorporated with 0.07 μg/cm2 of enotmocidus showed insecticidal activity against S. littoralis with LC50 of 16.4 and 21.9 μg/cm2. The E. coli lysates containing GST‐CryIC and its 3′‐truncated version showed low toxicity against the lepidopteran insect (10.8 and 16.6 μg/cm2) compared to 0.15 μg/cm2 of the native crystalline form of CryIC. Similarly, the mosquitocidal activity of the recombinant Bt toxins was low.

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“…It can be seen that vector surveillance and control strategies are mainly targeting the larval breeding sites. This includes the use of chemicals ( Lee et al, 1997 ; Sulaiman et al, 2000 , 2002 ; Chung et al, 2001 ; Tun-Lin et al, 2009 ; Huy et al, 2010 ; Oo et al, 2011 ; Kittayapong et al, 2012 ; Saiful et al, 2012 ; Sommerfeld and Kroeger, 2012 ), biological agents ( Seng et al, 2008a ; Lacroix et al, 2012 ; Hugo et al, 2014 ; Lazaro et al, 2015 ; Zuharah et al, 2015 ), environmental management ( Ooi et al, 2006 ; Tun-Lin et al, 2009 ; Lee et al, 2013 ; Lau et al, 2015 ), and community participation ( Chang et al, 2011 ). We have become over-dependent on chemicals and now the Aedes mosquitoes are resistant to most pyrethroids ( Ponlawat et al, 2005 ; Wan-Norafikah et al, 2010 ; Koou et al, 2014a , b ; Ishak et al, 2015 ).…”

Section: Vector Control Measuresmentioning

confidence: 99%

New Paradigms for Virus Detection, Surveillance and Control of Zika Virus Vectors in the Settings of Southeast Asia

et al. 2016

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Zika virus (ZIKV) has now become a global public health concern. The vectors for ZIKV are Aedes aegypti and A. albopictus. Both these mosquitoes are predominant in Southeast Asia and are also responsible for the spread of other arboviral diseases like dengue virus and chikungunya virus. The incidence of dengue has been increasing over the years and this is of concern to public health workers. Simple laboratory tools for the detection of ZIKV is also lacking. In the absence of drugs and vaccine for these arboviral diseases, vector control is the main option for surveillance and control. Aedes larval surveys have been the hallmark of dengue control along with larviciding and fogging when cases are reported. However, we need new paradigms and options for control of these vectors. The current situation in Southeast Asia clearly proves that effective strategies for vector control need to be proactive and not reactive. This will be the way forward to control epidemics of these diseases inclusive of ZIKV until a vaccine becomes available.

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Residual effects of TMOF–Bti formulations against 1st instar Aedes aegypti Linnaeus larvae outside laboratory

Cited by 8 publications

References 8 publications

Efficacy of Bacillus thuringiensis Treatment on Aedes Population Using Different Applications at High-Rise Buildings

Efficacy of Bacillus thuringiensis Treatment on Aedes Population Using Different Applications at High-Rise Buildings

Synergistic activity of Bacillus thuringiensis δ‐endotoxin and TMOF against Culex pipiens and Spodoptera littoralis larvae

New Paradigms for Virus Detection, Surveillance and Control of Zika Virus Vectors in the Settings of Southeast Asia

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