Digestive cells from Mytilus galloprovincialis show a partial regulatory volume decrease following acute hypotonic stress through mechanisms involving inorganic ions

Abstract

The response of isolated digestive cells of the digestive gland of Mytilus galloprovincialis to hypotonic shock was studied using videometric methods. The isolated cells exposed to a rapid change (from 1100 to 800 mosmol kg(-1) ) of the bathing solution osmolality swelled but thereafter underwent a regulatory volume decrease (RVD), tending to recover the original size. When the hypotonic stress was applied in the presence of quinine and glibenclamide, known inhibitors of swelling activated ion channels, the cells did not exhibit an RVD response; in addition, they showed a larger increase in size in respect to control cells. These observations suggest that the digestive cells of the digestive gland have the machinery to cope with the hyposmotic shock allowing them to exhibit a small but significant RVD preventing an excessive increase in cell size. The pharmacological treatment of digestive cells during the RVD experiments suggests that cell volume is regulated by K(+) and Cl(-) efflux followed by an obliged water efflux from the cell. The involvement of organic osmolytes such as taurine and betaine seems to be excluded by NMR measurement on digestive cells.

Osmoregulated Chloride Currents in Hemocytes from Mytilus galloprovincialis

“…In many cell types, including bivalves [35], this behaviour is typically mediated by potassium and chloride electroneutral co-transport as well as VRAC (Volume-Regulated Anion Channels) which are typically inactive under resting conditions, but are able to contribute to a partial recovery of the cell size by a regulatory volume decrease mechanism in cells subjected to hypotonic stress [36–38]. …”

“…For example, by using videometric methods it has been demonstrated that the cells from the digestive glands of M . galloprovincialis , exposed to rapid changes of the bathing solution (from 1100 to 800 mosmol/kg), undergo to a process of regulatory volume decrease [35]. Possibly the minor movements of M .…”

“…It is well recognized that potassium and chloride transport [38] typically contribute to RVD in several cell types and specifically in immunocells, such as thymocytes from rats [59] and mice [60] as well as lymphoblastic leukemia cells [44]. Interestingly it has also been suggested that in Mytilus galloprovincialis digestive cells [35] and in Mytilus californianus gill cells [61] K + and Cl _ cooperate in RVD by the efflux of these two ions followed by an obliged efflux of water from the cell.…”

The metabolic profile of lemon juice by proton HR-MAS NMR: the case of the PGI Interdonato Lemon of Messina

“…In order to identify and quantify the different metabolites that determine and characterise a precise foodstuff in its particular state, the approach that seems the most reliable is that of Metabolomics (Lindon et al 2007). The metabolism represents the physical and chemical reactions that occur in a living organism, so that its metabolic content is important for a comprehensive analysis of all metabolite changes upon stimuli (Lindon et al 2007) or stress conditions (Torre et al 2013). Indeed, the knowledge of the metabolite content can be used for the characterisation of a given foodstuff in its particular state.…”

Preliminary Study on the In vitro and In vivo Effects of Asparagopsis taxiformis Bioactive Phycoderivates on Teleosts

“…This regulatory response is called “regulatory volume decrease” (RVD). Volume-regulatory behavior has been observed in: cells isolated from digestive glands of Mytilus galloprovincialis (Torre et al, 2013), Gobius niger enterocytes (Trischitta et al, 2004), Anguilla anguilla enterocytes (Trischitta et al, 2005), turbot hepatocytes (Ollivier et al, 2006), Ehrlich ascites tumour cells (Hoffmann, 1984), and mammalian red blood cells (Ellory et al, 1985) exposed to osmotic stress.…”