Digestive Enzyme Activity during Initial Ontogeny and after Feeding Diets with Different Protein Sources in Zebra Cichlid, <i>Archocentrus nigrofasciatus</i>

Mente, Eleni; Solovyev, Mikhail; Vlahos, Nikolaos; Rotllant, Guiomar; Gisbert, Enric

doi:10.1111/jwas.12381

Cited by 11 publications

(5 citation statements)

References 59 publications

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“…After the samples have been deparaffinized, the sections were rehydrated, then stained with Hematoxylin-Eosin and mounted with Cristal/Mount and were examined for alterations with a microscope (Axiostar plus Carl Zeiss Light Microscopy, Carl Zeiss Ltd., Gottingen, Germany) under a total magnification of 100× and 400×. A semi-quantitative grading system was used in order to quantify the histopathological alterations of the examined tissues [34]. The severity grading used the following system: Grade 0 (not remarkable), Grade 1 (minimal), Grade 2 (mild), Grade 3 (moderate), Grade 4 (severe).…”

Section: Abiotic Factorsmentioning

confidence: 99%

An Experimental Brackish Aquaponic System Using Juvenile Gilthead Sea Bream (Sparus aurata) and Rock Samphire (Crithmum maritimum)

et al. 2019

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Brackish aquaponics using Mediterranean fish and plants provides an alternative opportunity for a combined production of high-quality food products with high commercial and nutritional value. This is the first study that investigates the effect of two different salinities (8 and 20 ppt) on growth and survival of Sparus aurata and Crithmum maritimum along with the cellular stress pathways using the activation of heat shock proteins (HSPs) and mitogen-activated protein kinase (MAPK) protein family members and the water bacterial abundance. In total, 156 fish were used (average initial weight of 2.55 g, length of 5.57 cm) and 36 plants (average initial height of 8.23 cm) in floating racks above the 135 L fish tanks. Survival rate for both organisms was 100%. C. crithmum grew better at 8 ppt (t-test, p < 0.05). The growth rate of S. aurata was similar for both treatments (p > 0.05). HSPs and MAPK were differentially expressed, showing tissue-specific responses. The average bacterial abundance at the end of the experiment was higher (p < 0.05) in the 20 ppt (18.6 ± 0.91 cells × 105/mL) compared to the 8 ppt (6.8 ± 1.9 cells × 105/mL). The results suggest that the combined culture of euryhaline fish and halophytes provides good quality products in brackish aquaponics systems.

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Section: Abiotic Factorsmentioning

confidence: 99%

An Experimental Brackish Aquaponic System Using Juvenile Gilthead Sea Bream (Sparus aurata) and Rock Samphire (Crithmum maritimum)

et al. 2019

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“…Therefore, identifying the optimum conditions and functional properties of proteases would allow better prediction of protein digestibility (Alarcón, Díaz, Moyano, & Abellán, 1998; Glass, McDonald, Moran, & Stark, 1989; Xiong, Xie, Zhang, & Liu, 2011). This fundamental information on digestive physiology is useful in understanding the nutritional requirements and feed digestion, absorption and metabolism, leading to effective feed formulation to enhance performance (Haard, Dimes, Arndt, & Dong, 1996; Kolkovski, 2001) and reduce feed waste, environmental impact and feed cost (Mente, Solovyev, Vlahos, Rotllant, & Gisbert, 2016).…”

Section: Introductionmentioning

confidence: 99%

Digestive proteases and in vitro protein digestibility in bagrid catfish Mystus nemurus (Cuvier and Valenciennes 1840)

2020

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Digestive proteases in the stomach and intestine of bagrid catfish Mystus nemurus juveniles and in vitro protein digestibility of fishmeal (FM), soya bean meal (SBM) and squid meal (SM) were determined. Postprandial data demonstrated highest protease activity 2 and 5 hr in the stomach and intestine, respectively, after feeding. Acidic-like protease in the stomach exhibited an optimum activity at pH 2, while alkaline proteases dominated the intestine, with optimum activities between pH 9 and 11. Investigations using specific commercial inhibitors confirmed the presence of pepsin in the stomach. Chymotrypsin, trypsin and metalloproteases were present in the intestine; however, the results indicated a possible involvement of other serine proteases. The highest inhibition of intestinal protease activity of bagrid catfish occurred during incubation with SM, followed by SBM and FM. Fishmeal had the highest relative protein digestibility (RPD), compared with SBM and SM, regardless of enzyme system used. Crude enzyme extract and the 4E system resulted in the highest RPD followed by 3E and 1E systems. The current results suggest that bagrid catfish adopt the typical protein digestion pathway of a carnivorous fish of endoprotease and exoprotease activities from the stomach to the intestine. Pepsin acts in the stomach, while trypsin, chymotrypsin, metalloproteases and other serine proteases are involved in the intestine. This study also recommends the use of crude intestinal enzyme extract for digestibiliy studies, instead of commercial enzyme systems, to better identify the suitable protein sources for bagrid catfish.

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“…In this regard, the live feed is one of the essential factors to increase the growth performance of larvae due to its excellent supply source of carbohydrate, lipid, and protein (Conceicao et al 2010). The high protein contained feed is required for farming ornamental fish that enhanced the production cost (Mente et al 2017).…”

Section: Introductionmentioning

confidence: 99%

A model for tubificid worm (Tubifex tubifex) production and its effect on growth of three selected ornamental fish

ALAM¹,

KHAN²,

SAROWER-E-MAHFUJ³

et al. 2022

Bangladesh J. Fish.

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. The demand for sludge worms, Tubifex tubifex is increasing with the growth of aquaculture as low-cost live feed in hatcheries and ornamental fish nurseries. The study was conducted to determine the optimal culture medium for T. tubifex worm’s production and to find out the inclusion effects of worms as feed on ornamental fish rearing. Three media-based treatments (T-1: mustard oil cake 20%, wheat bran 10%, soybean meal 10%, rice bran 20%, cow-dung 20%, sand 10%, fish pond mud 10%; T-2: mustard oil cake 10%, wheat bran 10%, soybean meal 10%, rice bran 10%, straw 5%, fish scale, stomach and fin 25%, cow-dung 20%, fish pond mud 10% and T-3: cow-dung 75%, sand 15%, fish pond mud 10%) with three replications of each were used to culture the worms for 100 days in nine cemented culvert system (160×25×10 cm3). The growth performance of three popular ornamental fishes koi carp (Cyprinus carpio), comet goldfish (Carassius auratus auratu) and goldfish (Carassius auratus) were determined by replacing the commercial feed at different level with tubificid rearing in fiberglass aquarium (45×30×30 cm3) for 32 days duration. Maximum yield (959.23 44.34 mg·cm-2) of tubificid biomass was recorded in T-2 (mustard oil cake-10%, wheat bran-10%, soybean meal-10%, rice bran-10%, straw-5%, fish scale, intestine, stomach and fin-25%, cow-dung-20%, mud collected from fish pond-10%) with a peak at day 70th. The fish fed with 50% commercial feed + 50% T. tubifex in TF-2 showed significantly (p<0.05) higher growth and survival performance than other treatments. The mean value of water quality parameters (i.e. temperature, pH and dissolved oxygen) were statistically insignificant (p>0.05) among the three treatments. Approximately 1.83 kg media were needed to produce 1.0 kg tubificid worm in treatment T-2. T-2 media is recommended as suitable media for commercial tubificid worm production and the inclusion of 50 % tubificid worms in the feed can be suggested for the ornamental fish rearing.

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Digestive Enzyme Activity during Initial Ontogeny and after Feeding Diets with Different Protein Sources in Zebra Cichlid, Archocentrus nigrofasciatus

Cited by 11 publications

References 59 publications

An Experimental Brackish Aquaponic System Using Juvenile Gilthead Sea Bream (Sparus aurata) and Rock Samphire (Crithmum maritimum)

An Experimental Brackish Aquaponic System Using Juvenile Gilthead Sea Bream (Sparus aurata) and Rock Samphire (Crithmum maritimum)

Digestive proteases and in vitro protein digestibility in bagrid catfish Mystus nemurus (Cuvier and Valenciennes 1840)

A model for tubificid worm (Tubifex tubifex) production and its effect on growth of three selected ornamental fish

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