Digestive enzymes and metabolic profile of Labeo rohita fingerlings fed diets with different crude protein levels

Debnath, Dipesh; Pal, A. K.; Sahu, Narottam Prasad; Yengkokpam, Sona; Baruah, Kartik; Choudhury, Dharitri; Venkateshwarlu, G.

doi:10.1016/j.cbpb.2006.09.008

Cited by 153 publications

(132 citation statements)

References 40 publications

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“…HSI value can be correlated with the amount of fat or glycogen deposition (Gao et al, 2012;Debnath et al, 2007). In the present study, significantly higher HSI value was recorded in the control and RPI 50 fed groups compared to others, which indicate higher lipid deposition in the liver.…”

Section: Discussionsupporting

confidence: 54%

“…The fish were anaesthetized with clove oil (50 L of clove oil per litre of water; Debnath et al, 2007) and blood was collected from the caudal vein without anticoagulant into a dried eppendorf tube and allowed to stand in a slanted position at room temperature. The blond were centrifuged at 5000g for 10 min in a cooling centrifuge (REMI CPR-24, India), and transferred into another eppendorf tube and kept in − 20 • C until use.…”

Section: Serum Biochemistry and Enzyme Assaymentioning

confidence: 99%

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Nutritional evaluation of protein isolate from rubber seed in the diet of Labeo rohita : Effects on growth performance, nutrient utilization, whole body composition and metabolic enzymes activity

Fawole

Sahu

Jain

et al. 2016

Animal Feed Science and Technology

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a b s t r a c tThe nutritional potential of protein isolate from rubber seed (RPI) in the diets of Labeo rohita (initial average weight 4.45 ± 0.01 g) was assessed in a 60 days feeding trial. Five isonitrogenous (32.62 ± 0.13 CP Kg −1 ) and isocaloric (18.47 ± 0.08 MJ kg −1 ) experimental diets were formulated with graded level of RPI like 0%, 25%, 50%, 75%, or 100% in replacement for soybean protein isolate (SPI), and designated as Control, RPI25, RPI50, RPI75, RPI100, respectively. The RPI contributed 0%, 13%, 26%, 39% or 52% of the total dietary protein in the diets. Each diets were randomly assigned to 15 experimental tanks containing 12 fish in triplicates and fed to satiation twice daily at 10:00 h and 18:00 h. At the end of the feeding trial, the growth performance and nutrient utilization indices such as percent weight gain (WG%), specific growth rate (SGR), daily growth coefficient (DGC), feed intake (FI), protein efficiency ratio (PER), feed conversion ratio (FCR) and protein retention (PR) values were not significantly (p > 0.05) affected by the dietary treatments irrespective of inclusion levels of RPI. A significantly higher (p < 0.05) hepatosomatic index (HSI) was recorded in the control and RPI 50 group compared to other treatment groups (p < 0.05). The intestinal somatic index (ISI) and Survival rate were similar (p > 0.05) in all the groups. The apparent digestibility coefficients (ADCs) of dry matter and protein for fish fed the control and RPI 100 diets were found to be similar, while RPI 50 and RPI 75 groups exhibited a significantly lower value corresponding to the protease enzyme activity. The whole body compositions and digestive/metabolic enzymes activities among the various groups did not differ significantly (p > 0.05). The serum cholesterol and triglyceride levels were found to be significantly higher (p < 0.05) in the control compared to the RPI fed groups. Significantly higher serum glucose level was recorded in RPI 50, while a reverse was seen in the liver glycogen contents. Overall, this study clearly showed that RPI from rubber seed can serve as alternative protein source in the diets of L. rohita fingerlings without any adverse effects on growth, nutrient utilization and physio-metabolic responses.

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Section: Discussionsupporting

confidence: 54%

Section: Serum Biochemistry and Enzyme Assaymentioning

confidence: 99%

Nutritional evaluation of protein isolate from rubber seed in the diet of Labeo rohita : Effects on growth performance, nutrient utilization, whole body composition and metabolic enzymes activity

Fawole

Sahu

Jain

et al. 2016

Animal Feed Science and Technology

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“…In trout, amylase activity increases with higher consumption, temperature, and water salinity (Steffens, 1987). No variation was observed in amylase activities, even in diets with lower protein and high carbohydrate levels, in fingerling Labeo rohita fed diets containing different protein levels (25 up to 40% CP) (Debnath et al, 2007).…”

Section: Discussionmentioning

confidence: 86%

“…High HSI values are usually found in fish fed diets containing high carbohydrate levels (Debnath et al, 2007). When the protein level is lower, other nutrients may be used for energy production, resulting in higher glycogen in the hepatic tissue, with increased HSI.…”

Section: Discussionmentioning

confidence: 99%

Protein sources and digestive enzyme activities in jundiá (Rhamdia quelen)

Lazzari

Neto

Pedron

et al. 2010

Sci. agric. (Piracicaba, Braz.)

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Digestive enzymes activity influence feed utilization by fish, and its understanding is important to optimize diet formulation. This study reports the digestive enzyme activities of jundiá juveniles fed diets with protein sources. Fish were fed six experimental diets for 90 days: MBY (meat and bone meal + sugar cane yeast), SY (soybean meal + sugar cane yeast), S (soybean meal), MBS (meat and bone meal + soybean meal), FY (fish meal + sugar cane yeast) and FS (fish meal + soybean meal), and then sampled every 30 days and assayed in two intestine sections for digestive enzymes -trypsin, chymotrypsin and amylase -activities; gastric protease was assayed in the stomach. Digestive and hepatosomatic index, intestinal quotient, digestive tract length and weight gain were also measured. Trypsin and chymotrypsin activities were higher (p < 0.0001) in fish fed diets containing animal protein sources (MBY, MBS, FY and FS diets). Alkaline proteases were negatively affected by dietary soybean meal in the SY and S diets. Amylase activity had greater variation between diets and intestine sections. Fish fed MBY and MBS diets showed higher gastric protease activity (p < 0.0001). Weight gains were higher in fish fed the MBS and FS diets (p < 0.05). Highest values (p < 0.05) of digestive tract length were observed in fish fed MBS and FY diets, but no correlation with enzymes activities was found. Hepatosomatic index (HSI) was affected by diet composition. Dietary composition influenced digestive enzymes activities of jundiá. Key words: digestion, nutrition, soybean meal, meat and bone meal, fish mealFontes protéicas e atividade de enzimas digestivas em jundiás (Rhamdia quelen) RESUMO: As enzimas digestivas influenciam a utilização dos alimentos em peixes, e seu conhecimento é importante para otimizar a formulação de dietas. Este trabalho descreve a atividade de enzimas digestivas em juvenis de jundiá alimentados com fontes protéicas. Os peixes foram alimentados com seis dietas (90 dias): MBY (farinha de carne e ossos + levedura de cana), SY (farelo de soja + levedura de cana), S (farelo de soja), MBS (farinha de carne e ossos + farelo de soja), FY (farinha de peixe + levedura de cana) e FS (farinha de peixe + farelo de soja). A cada 30 dias, foram analisadas as enzimas digestivas (tripsina, quimiotripsina e amilase) no intestino. No estômago, foi mensurada a protease ácida. Foram estimados os índices digestivo e hepato-somático, quociente intestinal, comprimento do trato digestório e ganho em peso.

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“…Therefore, 25% fishmeal replaced with T. ornata and G. corticata have more influences on growth and nutritional profiles of M. rosenbergii due to the enhanced activities of digestive enzymes, as they have been reported to regulate the growth and moult cycle directly or indirectly (Lovett and Felder, 1990;Sun et al, 2011). Actually, crustaceans can able to digest a variety of complex food materials as they contain high concentration of protein, carbohydrate and lipid digesting enzymes, such as pepsin, trypsin, chymotrypsin, carboxypeptidases-A and B, leucine, aminopeptidase, amylase, collagenase, esterase and lipase (Gamboa-Delgado et al, 2003;Debnath et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Partial Replacement of Fishmeal with Marine Algae Turbinaria ornata and Gracilaria corticata for Sustainable Culture of the Freshwater Prawn Macrobrachium rosenbergii

Rajkumar¹,

Bhavan²,

Srinivasan³

et al. 2017

International Journal of Research Studies in Zoology

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Digestive enzymes and metabolic profile of Labeo rohita fingerlings fed diets with different crude protein levels

Cited by 153 publications

References 40 publications

Nutritional evaluation of protein isolate from rubber seed in the diet of Labeo rohita : Effects on growth performance, nutrient utilization, whole body composition and metabolic enzymes activity

Nutritional evaluation of protein isolate from rubber seed in the diet of Labeo rohita : Effects on growth performance, nutrient utilization, whole body composition and metabolic enzymes activity

Protein sources and digestive enzyme activities in jundiá (Rhamdia quelen)

Partial Replacement of Fishmeal with Marine Algae Turbinaria ornata and Gracilaria corticata for Sustainable Culture of the Freshwater Prawn Macrobrachium rosenbergii

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