Digestive enzymes in juvenile green abalone, Haliotis fulgens, fed natural food

García‐Carreño, Fernando; Toro, M. A. Navarrete del; Servière‐Zaragoza, Elisa

doi:10.1016/s1096-4959(02)00221-x

Cited by 35 publications

(27 citation statements)

References 20 publications

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“…They are characterized by an active site consisting of histidine, aspartate and serine as the nucleophilic residue to attack the scissile peptide bond in proteins. In several previous studies, we found that not the entire proteolytic activities in the gastric juice of crustaceans could be assigned to serine proteinases, either because total activity was not entirely inhibited by specific inhibitors or because significant activity was detected at acid pH (García-Carreño et al, 2003;Celis-Guerrero et al, 2004).…”

Section: Discussionmentioning

confidence: 85%

Aspartic proteinases in the digestive tract of marine decapod crustaceans

et al. 2006

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Decapod crustaceans synthesize highly active proteolytic enzymes in the midgut gland and release at least a part of them into the stomach where they facilitate the first step in peptide hydrolysis. The most common proteinases in the gastric fluid characterized so far are serine proteinases, that is, trypsin and chymotrypsin. These enzymes show highest activities at neutral or slightly alkaline conditions. The presence of acid proteinases, as they prevail in vertebrates, has been discussed contradictorily yet in invertebrates. In this study, we show that acid aspartic proteinases appear in the gastric fluid of several decapods. Lobsters Homarus gammarus showed the highest activity with a maximum at pH 3. These activities were almost entirely inhibited by pepstatin A, which indicates a high share of aspartic proteinases. In other species (Panulirus interruptus, Cancer pagurus, Callinectes arcuatus and Callinectes bellicosus), proteolytic activities were present at acid conditions but were distinctly lower than in H. gammarus. Zymograms at pH 3 showed in each of the studied species at least one, but mostly two-four bands of activity. The apparent molecular weight of the enzymes ranged from 17.8 to 38.6 kDa. Two distinct bands were identified which were inhibited by pepstatin A. Acid aspartic proteinases may play an important role in the process of extracellular digestion in decapod crustaceans. Activities were significantly higher in clawed lobster than in spiny lobster and three species of brachyurans. Therefore, it may be suggested that the expression of acid proteinases is favored in certain groups and reduced in others.

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Section: Discussionmentioning

confidence: 85%

Aspartic proteinases in the digestive tract of marine decapod crustaceans

et al. 2006

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“…By showing the APTT activity of recombinant AbAntistasin, it could be suggested that Ab-Antistasin has low anticoagulant activity towards human blood plasma. In invertebrates, a cascade of serine protease zymogens leading to hemolymph coagulation has been described [51,52]. Since there is no evidence of an abalone blood coagulation system to our knowledge, it could be suggested further that this may be the reason for the lower anticoagulant activity of the protein.…”

Section: Resultsmentioning

confidence: 99%

Disk abalone (Haliotis discus discus) expresses a novel antistasin-like serine protease inhibitor: Molecular cloning and immune response against bacterial infection

Nikapitiya

Zoysa

et al. 2010

Fish & Shellfish Immunology

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“…The natural diet of H. midae consists primarily of the kelp species Ecklonia maxima, which is harvested from the west and south-west coasts of South Africa (Tarr 2000a, Troell et al 2006. The haliotid digestive system is anatomically and biochemically suited to feed on macroalgae, and is well suited for their carbohydratebased metabolism (Carefoot et al 2000, Picos-García et al 2000, García-Carreño et al 2003. In recent years, there has been a rapid increase in the development of artificial feeds to supplement or replace macroalgae in abalone aquaculture (Fleming et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Exploring basic biochemical constituents in the body tissues of South African abaloneHaliotis midaereared in shore-based mariculture systems

Laas

Vosloo

2010

African Journal of Marine Science

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The study quantifies the effects of season, age and feeding regime on glucose, glycogen, proteins and lipids in abalone Haliotis midae tissues, and to assess water loss during simulated live export. Abalone of two age classes, younger (~30 months old) and older (~38 months old), were subjected to four dietary regimes: two single feeds, kelp and an artificial feed, and two rotational feeding regimes utilising kelp and the artificial feed in varying ratios. Total protein, free glucose, glycogen and total lipid content were determined in the digestive gland and foot/adductor muscle. One subset of older animals, from each feeding treatment, was subjected to water content analysis by drying. Subsets of older and younger animals were subjected to a simulated live export trial to determine mass loss. Digestive gland tissue proved to be of little value for routine monitoring. Muscle glucose, muscle lipid and muscle protein concentrations were affected by month and age. Muscle glycogen concentration was affected by month, age and feeding regime. During simulated export trials, animals lost on average 15% body mass, being significantly higher (p < 0.01) in younger compared to older animals. Feeding regime did not influence susceptibility to mass loss during export. Animal age influenced the biochemical composition and export performance of abalone.

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Digestive enzymes in juvenile green abalone, Haliotis fulgens, fed natural food

Cited by 35 publications

References 20 publications

Aspartic proteinases in the digestive tract of marine decapod crustaceans

Aspartic proteinases in the digestive tract of marine decapod crustaceans

Disk abalone (Haliotis discus discus) expresses a novel antistasin-like serine protease inhibitor: Molecular cloning and immune response against bacterial infection

Exploring basic biochemical constituents in the body tissues of South African abaloneHaliotis midaereared in shore-based mariculture systems

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