Digging deeper: structural background of PEGylated fibrin gels in cell migration and lumenogenesis

Shpichka, Anastasia; Konarev, Petr V.; Efremov, Yuri M.; Kryukova, A. E.; Aksenova, N. A.; Kotova, Svetlana; Frolova, Anastasia; Kosheleva, Nastasia; Жигалина, О. М.; Yusupov, V. I.; Khmelenin, D. N.; Koroleva, Anastasia; Волков, В. В.; Асадчиков, В. Е.; Timashev, Peter

doi:10.1039/c9ra08169k

Cited by 29 publications

(42 citation statements)

References 48 publications

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“…Regarding bioinks, the most promising cell components are spheroids or organoids establish intercellular junctions and newly synthetized ECM compared to a single cell suspension and maintain cell phenotype[ 22 , 104 - 106 ]; the biomaterial component – hydrogel system – is usually presented by natural and synthetic polymers, including their conjugates such as acellularized ECM, alginate, gelatin, fibrin, hyaluronic acid, cellulose, polyethylene glycol, and Pluronic-F127. [ 101 , 107 - 109 ].…”

Section: Designing An “Ideal” Tissue Model To Study Sars-cov-2 Infementioning

confidence: 99%

Engineering a Model to Study Viral Infections: Bioprinting, Microfluidics, and Organoids to Defeat Coronavirus Disease 2019 (COVID-19)

Shpichka¹,

Bikmulina²,

Peshkova³

et al. 2024

IJB

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While the number of studies related to severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) is constantly growing, it is essential to provide a framework of modeling viral infections. Therefore, this review aims to describe the background presented by earlier used models for viral studies and an approach to design an “ideal” tissue model for SARS-CoV-2 infection. Due to the previous successful achievements in antiviral research and tissue engineering, combining the emerging techniques such as bioprinting, microfluidics, and organoid formation are considered to be one of the best approaches to form in vitro tissue models. The fabrication of an integrated multi-tissue bioprinted platform tailored for SARS-CoV-2 infection can be a great breakthrough that can help defeat coronavirus disease in 2019.

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Section: Designing An “Ideal” Tissue Model To Study Sars-cov-2 Infementioning

confidence: 99%

Engineering a Model to Study Viral Infections: Bioprinting, Microfluidics, and Organoids to Defeat Coronavirus Disease 2019 (COVID-19)

Shpichka¹,

Bikmulina²,

Peshkova³

et al. 2024

IJB

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“…The main parameter that controls the gel stiffness appears to be fibrinogen concentration. By varying it in a range from 1 to 50 mg/mL, the elastic modulus of the resulted gel from several Pa to several hundred Pa might be achieved[ 27 - 29 ]. Another important modulator is Factor XIII, its addition substantially increases the elastic modulus of the gel by incorporating fibrin covalent crosslinking and compacting fibers[ 6 ].…”

Section: Fibrin Overviewmentioning

confidence: 99%

“…Combinations with gelatin[ 33 ], alginate[ 34 ], collagen[ 35 ], hyaluronic acid[ 36 ], or more complex formulations[ 37 ] were used for different applications. Some biochemical modifications were also introduced to the fibrinogen to modulate the structural and mechanical properties of the gel[ 27 , 29 , 38 ].…”

Section: Fibrin Overviewmentioning

confidence: 99%

“…Its fibrillar structure serves as a scaffold for invading cells which bind to its fibers through cellular receptors and form capillaries. It was showed that the fiber network morphology can be significantly influenced by fibrinogen and thrombin concentrations[ 65 - 67 ], pH[ 68 ], buffers[ 5 ], incorporation of extra molecules[ 27 ], etc.…”

Section: Biological Properties and Their Tuningmentioning

confidence: 99%

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Fibrin-based Bioinks: New Tricks from an Old Dog

Shpichka¹,

Osipova²,

Efremov³

et al. 2024

IJB

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For the past 10 years, the main efforts of most bioprinting research teams have focused on creating new bioinkformulations, rather than inventing new printing set-up concepts. New tissue-specific bioinks with good printability, shapefidelity, and biocompatibility are based on “old” (well-known) biomaterials, particularly fibrin. While the interest in fibrinbased bioinks is constantly growing, it is essential to provide a framework of material’s properties and trends. This review aimsto describe the fibrin properties and application in three-dimensional bioprinting and provide a view on further developmentof fibrin-based bioinks

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“…Stocks were stored at −20°C before use. The used modification of fibrinogen was previously described 7,55,56 and performed at a day of experiment by adding 1.5 mg∕mL solution of O,O′-bis[2-(N-succinimidyl-succinylamino)ethyl]polyethylene glycol (PEG-NHS; Sigma-Aldrich, Germany) in PBS at a molar ratio of 5:1 (PEG-NHS: fibrinogen). The reaction of PEGylation was carried out for 2 h at 37°C.…”

Section: Hydrogel Components and Formationmentioning

confidence: 99%

Beyond 2D: effects of photobiomodulation in 3D tissue-like systems

et al. 2020

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Significance: Currently, various scaffolds with immobilized cells are widely used in tissue engineering and regenerative medicine. However, the physiological activity and cell viability in such constructs might be impaired due to a lack of oxygen and nutrients. Photobiomodulation (PBM) is a promising method of preconditioning cells to increase their metabolic activity and to activate proliferation or differentiation. Aim: Investigation of the potential of PBM for stimulation of cell activities in hydrogels. Approach: Mesenchymal stromal cells (MSCs) isolated from human gingival mucosa were encapsulated in modified fibrin hydrogels with different thicknesses and concentrations. Constructs with cells were subjected to a single-time exposure to red (630 nm) and near-infrared (IR) (840 nm) low-intensity irradiation. After 3 days of cultivation, the viability and physiological activity of the cells were analyzed using confocal microscopy and a set of classical tests for cytotoxicity. Results: The cell viability in fibrin hydrogels depended both on the thickness of the hydrogels and the concentration of gel-forming proteins. The PBM was able to improve cell viability in hydrogels. The most pronounced effect was achieved with near-IR irradiation at the 840-nm wavelength. Conclusions: PBM using near-IR light can be applied for stimulation of MSCs metabolism and proliferation in hydrogel-based constructs with thicknesses up to 3 mm.

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Digging deeper: structural background of PEGylated fibrin gels in cell migration and lumenogenesis

Abstract: Fibrin is a well-known tool in tissue engineering, but the structure of its modifications created to improve its properties remains undiscussed despite their importance, e.g. in designing biomaterials that ensure cell migration and lumenogenesis.

Cited by 29 publications

References 48 publications

Engineering a Model to Study Viral Infections: Bioprinting, Microfluidics, and Organoids to Defeat Coronavirus Disease 2019 (COVID-19)

Engineering a Model to Study Viral Infections: Bioprinting, Microfluidics, and Organoids to Defeat Coronavirus Disease 2019 (COVID-19)

Fibrin-based Bioinks: New Tricks from an Old Dog

Beyond 2D: effects of photobiomodulation in 3D tissue-like systems

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