Digital enzyme-linked immunosorbent assays with sub-attomolar detection limits based on low numbers of capture beads combined with high efficiency bead analysis

Abstract

We report approaches to improve the sensitivity of digital ELISA up to 400-fold, enabling detection of proteins at subattomolar concentrations.

A critical review: Recent advances in “digital” biomolecule detection with single copy sensitivity

“…(g) The scheme of MMS. Reprinted with permission from ( Kan et al, 2020 ). Copyright 2020 The Royal Society of Chemistry.…”

“…To further improve the sensitivity, each step of this analysis was systematically assessed and optimized, and a new generation platform named low bead digital ELISA was developed in 2020 ( Kan et al, 2020 ). The key step causing bead loss is “beads loaded and sealed in microwells”.…”

Aptamers for Detection and Diagnostics (ADD): Can mobile systems process optical data from aptamer sensors to identify molecules indicating presence of SARS-CoV-2 virus? Should healthcare explore aptamers as drugs for prevention as well as its use as adjuvants with antibodies and vaccines?

“…Sub-attomolar detection limits 530 are unlikely to be attained by ADD tools. Do we need such sensitivity for ADD as a global public health tool?…”

Aptamers for Detection and Diagnostics (ADD) is a proposed mobile app acquiring optical data from conjugated quantum nanodots to identify molecules indicating presence of SARS-CoV-2 virus: Why public health and healthcare need smartphone sensors as a platform for early detection and prevention

“…Sub-attomolar detection limits 523 are unlikely to be attained by ADD tools. Do we need such sensitivity for ADD as a global public health tool?…”