Digital image analysis of pan-cytokeratin stained tumor slides for evaluation of tumor budding in pT1/pT2 colorectal cancer: Results of a feasibility study

Jepsen, Rikke Karlin; Klarskov, Louise Laurberg; Lippert, Michael Friis; Novotny, Guy Wayne; Hansen, Tine Plato; Christensen, Ib Jarle; Høgdall, Estrid; Riis, Lene

doi:10.1016/j.prp.2018.07.002

Cited by 20 publications

(18 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides consultation and extending the assessment area, digital methods might be the most promising approach to support the assessment [5,15]. A semiautomatic approach has shown convincing results, whereas more advanced algorithms are being explored as well [16][17][18]. In particular, machine learning techniques will substantially contribute to solve the task, but the question of the "right" budding grade might then possibly be obsolete, too [19].…”

Section: Discussionmentioning

confidence: 99%

Interobserver Variability in the Assessment of Tumor Budding in pT 3/4 Colon Cancer: Improvement by Supporting Immunohistochemistry?

Martin¹,

Mayr

Ihringer³

et al. 2020

Diagnostics

View full text Add to dashboard Cite

The prognostic significance of tumor budding in colon cancer is unequivocally documented, and the recommendations of the International Tumor Budding Consensus Conference (ITBCC) are currently the accepted basis for its assessment. Up to now, it is unknown whether the general use of a supporting cytokeratin immunohistochemistry can improve the interobserver variability and prognostic significance. Six investigators with different levels of experience reassessed 229 cases of colon carcinoma (pT3/4, N+/−, M0) with a supporting cytokeratin immunohistochemistry. The results were compared to previous assessments, which have been performed only on H & E. Bd3 was significantly associated with the occurrence of distant metastases according to the assessments of three out of six investigators (p < 0.05). Only one single investigator reached significant results concerning the cancer specific survival (p = 0.01). The pairwise kappa values range between a poor and moderate level of agreement (range 0.17–0.45; median 0.21). In conclusion, the results show no superiority of the use of an additional cytokeratin immunohistochemistry compared to the conventional analysis on sole H & E slides. Therefore, the general supporting use of a cytokeratin immunohistochemical staining seems to be inadvisable in colon cancer in consideration of necessary resources and costs.

show abstract

Section: Discussionmentioning

confidence: 99%

Interobserver Variability in the Assessment of Tumor Budding in pT 3/4 Colon Cancer: Improvement by Supporting Immunohistochemistry?

Martin¹,

Mayr

Ihringer³

et al. 2020

Diagnostics

View full text Add to dashboard Cite

show abstract

“…Most semi‐automatic tumour scoring methods have been developed with immunohistochemically stained slides, 11,12,15–21 in some cases with immunofluorescent staining 20,22,23 . Only one detection system has been proposed for H&E‐stained slides 24 …”

Section: Automated Scoring Of Tumour Buds—where Do We Stand?mentioning

confidence: 99%

“…As tumour budding is best described in CRC, is it not surprising that most of the methods have also been developed with CRC tissue 11,12,15,17,18,20,21,23,24 . However, automation attempts have also been evaluated for early‐stage oral squamous cell carcinoma, 16 muscle‐invasive bladder cancer, 22 and breast tumours 19 …”

Section: Automated Scoring Of Tumour Buds—where Do We Stand?mentioning

confidence: 99%

Taking tumour budding to the next frontier — a post International Tumour Budding Consensus Conference (ITBCC) 2016 review

et al. 2020

View full text Add to dashboard Cite

Tumour budding in colorectal cancer, defined as single tumour cells or small clusters containing four or fewer tumour cells, is a robust and independent biomarker of aggressive tumour biology. On the basis of published data in the literature, the evidence is certainly in favour of reporting tumour budding in routine practice. One important aspect of implementing tumour budding has been to establish a standardised and evidence‐based scoring method, as was recommended by the International Tumour Budding Consensus Conference (ITBCC) in 2016. Further developments have aimed at establishing methods for automated tumour budding assessment. A digital approach to scoring tumour buds has great potential to assist in performing an objective budding count but, like the manual consensus method, must be validated and standardised. The aim of the present review is to present general considerations behind the ITBCC scoring method, and a broad overview of the current situation and challenges regarding automated tumour budding detection methods.

show abstract

“…2,12,37,54,59 High reproducibility has also been shown in many studies evaluating tumor budding in other cancers. 66,67 However, quantification of tumor budding based on digital image analysis still requires further investigation in HNSCC. Assessment of tumor budding using digital image analysis of pan-cytokeratin stained slides has recently shown a promising correlation with patient survival in oral cancer 25,65 and in colorectal cancer as well.…”

Section: Evaluation Of Tumor Buddingmentioning

confidence: 99%

“…Assessment of tumor budding using digital image analysis of pan-cytokeratin stained slides has recently shown a promising correlation with patient survival in oral cancer 25,65 and in colorectal cancer as well. 66,67 However, quantification of tumor budding based on digital image analysis still requires further investigation in HNSCC.…”

Section: Evaluation Of Tumor Buddingmentioning

confidence: 99%

Hallmarks of cancer: Tumor budding as a sign of invasion and metastasis in head and neck cancer

et al. 2019

View full text Add to dashboard Cite

Invasion and metastasis are hallmarks of cancer. The concept of tumor budding at tumor‐host interface has been documented in many carcinomas. A growing body of evidence indicates that tumor budding is a sign of invasion and early step for metastasis of many epithelial cancers including head and neck squamous cell carcinoma (HNSCC). In addition, recent research has underlined the importance of tumor budding as a promising prognosticator in HNSCC. This review summarizes the findings regarding tumor budding in HNSCC and focuses on the role of tumor budding in invasion and metastasis. Also, we highlight the prognostic significance of tumor budding in HNSCC and its potential for improving clinical decision making in terms of recommending optimal individualized treatment for this patient population.

show abstract

Digital image analysis of pan-cytokeratin stained tumor slides for evaluation of tumor budding in pT1/pT2 colorectal cancer: Results of a feasibility study

Cited by 20 publications

References 26 publications

Interobserver Variability in the Assessment of Tumor Budding in pT 3/4 Colon Cancer: Improvement by Supporting Immunohistochemistry?

Interobserver Variability in the Assessment of Tumor Budding in pT 3/4 Colon Cancer: Improvement by Supporting Immunohistochemistry?

Taking tumour budding to the next frontier — a post International Tumour Budding Consensus Conference (ITBCC) 2016 review

Hallmarks of cancer: Tumor budding as a sign of invasion and metastasis in head and neck cancer

Contact Info

Product

Resources

About