Digital Imaging Mass Spectrometry

Bamberger, Casimir; Renz, Uwe; Bamberger, A.

doi:10.1007/s13361-011-0120-1

Cited by 16 publications

(20 citation statements)

References 26 publications

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“…Pixelated detectors with a semiconductor sensor layer are used for velocity map imaging . An MCP in combination with a bare pixelated detector is used for MSI …”

Section: State‐of‐the‐art In Multi‐dimensional Ion Electron Photon mentioning

confidence: 99%

Detection systems for mass spectrometry imaging: A perspective on novel developments with a focus on active pixel detectors

Jungmann

Heeren

2012

Rapid Comm Mass Spectrometry

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Instrumental developments for imaging and individual particle detection for biomolecular mass spectrometry (imaging) and fundamental atomic and molecular physics studies are reviewed. Ion-counting detectors, array detection systems and high mass detectors for mass spectrometry (imaging) are treated. State-of-the-art detection systems for multidimensional ion, electron and photon detection are highlighted. Their application and performance in three different imaging modes -integrated, selected and spectral image detection -are described. Electro-optical and microchannelplate-based systems are contrasted. The analytical capabilities of solid-state pixel detectors -both charge coupled device (CCD) and complementary metal oxide semiconductor (CMOS) chips -are introduced. The Medipix/Timepix detector family is described as an example of a CMOS hybrid active pixel sensor. Alternative imaging methods for particle detection and their potential for future applications are investigated.

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“…Pixelated detectors with a semiconductor sensor layer are used for velocity map imaging . An MCP in combination with a bare pixelated detector is used for MSI …”

Section: State‐of‐the‐art In Multi‐dimensional Ion Electron Photon mentioning

confidence: 99%

Detection systems for mass spectrometry imaging: A perspective on novel developments with a focus on active pixel detectors

Jungmann

Heeren

2012

Rapid Comm Mass Spectrometry

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“…For details on the Medipix/ Timepix chips, available readout interfaces and various applications the interested reader is referred to [32] and references therein. The interested reader is referred to our initial publication [20] and [26] for more detail on the technical aspects of the TPX detection system and it's implications for MSI.…”

Section: Methodsmentioning

confidence: 99%

Biological Tissue Imaging with a Position and Time Sensitive Pixelated Detector

Jungmann

Smith

MacAleese

et al. 2012

J. Am. Soc. Mass Spectrom.

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We demonstrate the capabilities of a highly parallel, active pixel detector for large-area, mass spectrometric imaging of biological tissue sections. A bare Timepix assembly (512×512 pixels) is combined with chevron microchannel plates on an ion microscope matrix-assisted laser desorption time-of-flight mass spectrometer (MALDI TOF-MS). The detector assembly registers position-and time-resolved images of multiple m/z species in every measurement frame. We prove the applicability of the detection system to biomolecular mass spectrometry imaging on biologically relevant samples by mass-resolved images from Timepix measurements of a peptide-grid benchmark sample and mouse testis tissue slices. Mass-spectral and localization information of analytes at physiologic concentrations are measured in MALDI-TOF-MS imaging experiments. We show a high spatial resolution (pixel size down to 740×740 nm 2 on the sample surface) and a spatial resolving power of 6 μm with a microscope mode laser field of view of 100-335 μm. Automated, large-area imaging is demonstrated and the Timepix' potential for fast, large-area image acquisition is highlighted.

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“…The success of Ron Heeren's team in developing this technique has recently been confirmed by reports of other groups using this approach independently. 62,63 The microscope imaging MS could revolutionize the field by accelerating data acquisition by 2 orders of magnitude.…”

Section: ■ Emerging Technologies and Future Perspectivesmentioning

confidence: 99%

Serial 3D Imaging Mass Spectrometry at Its Tipping Point

Palmer

Alexandrov

2015

Anal. Chem.

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Since biology is by and large a 3-dimensional phenomenon, it is hardly surprising that 3D imaging has had a significant impact on many challenges in the life sciences. Imaging mass spectrometry (MS) is a spatially resolved label-free analytical technique that recently maturated into a powerful tool for in situ localization of hundreds of molecular species. Serial 3D imaging MS reconstructs 3D molecular images from serial sections imaged with mass spectrometry. As such, it provides a novel 3D imaging modality inheriting the advantages of imaging MS. Serial 3D imaging MS has been steadily developing over the past decade, and many of the technical challenges have been met. Essential tools and protocols were developed, in particular to improve the reproducibility of sample preparation, speed up data acquisition, and enable computationally intensive analysis of the big data generated. As a result, experimental data is starting to emerge that takes advantage of the extra spatial dimension that 3D imaging MS offers. Most studies still focus on method development rather than on exploring specific biological problems. The future success of 3D imaging MS requires it to find its own niche alongside existing 3D imaging modalities through finding applications that benefit from 3D imaging and at the same time utilize the unique chemical sensitivity of imaging mass spectrometry. This perspective critically reviews the challenges encountered during the development of serial-sectioning 3D imaging MS and discusses the steps needed to tip it from being an academic curiosity into a tool of choice for answering biological and medical questions.

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Digital Imaging Mass Spectrometry

Cited by 16 publications

References 26 publications

Detection systems for mass spectrometry imaging: A perspective on novel developments with a focus on active pixel detectors

Detection systems for mass spectrometry imaging: A perspective on novel developments with a focus on active pixel detectors

Biological Tissue Imaging with a Position and Time Sensitive Pixelated Detector

Serial 3D Imaging Mass Spectrometry at Its Tipping Point

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