Digital Microfluidics for Manipulation and Analysis of a Single Cell

He, Jiahao; Chen, An-Te; Lee, Jyong-Huei; Fan, Shih-Kang

doi:10.3390/ijms160922319

Cited by 59 publications

(33 citation statements)

References 102 publications

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“…These forces are caused by the potential difference between electrodes from an electrode array, which are coated with a dielectric and a hydrophobic insulator layer [99][100][101][102] on indium tin oxide (InSnO) glass or printed circuit boards (PCB). Electrowetting-on-dielectric (EWOD) digital microfluidic devices have been endorsed in many reports as a powerful platform for biological and biomedical research, including proteomic analysis [103][104][105][106], single-cell analysis [107][108][109][110], immunoassays [111], and clinical diagnostics [112].…”

Section: Electrowetting-on-dielectric (Ewod) Digital Microfluidic Devmentioning

confidence: 99%

Optofluidics 2015

2017

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Section: Electrowetting-on-dielectric (Ewod) Digital Microfluidic Devmentioning

confidence: 99%

Optofluidics 2015

2017

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“…To resolve this impediment, single cell analysis (SCA) has been introduced throughout the world to correct for the various disadvantages of those insensitive methods [1], especially in the time-lapse monitoring of dynamic cell changes [4,5]. So far, a vast majority of biomedical engineering tools (e.g., flow cytometry, optical tweezer, laser microdissection, and microfluidics) have facilitated single cell analysis [6,7].…”

Section: Introductionmentioning

confidence: 99%

A Potential Application of Triangular Microwells to Entrap Single Cancer Cells: A Canine Cutaneous Mast Cell Tumor Model

et al. 2019

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Cellular heterogeneity is a major hindrance, leading to the misunderstanding of dynamic cell biology. However, single cell analysis (SCA) has been used as a practical means to overcome this drawback. Many contemporary methodologies are available for single cell analysis; among these, microfluidics is the most attractive and effective technology, due to its advantages of low-volume specimen consumption, label-free evaluation, and real-time monitoring, among others. In this paper, a conceptual application for microfluidic single cell analysis for veterinary research is presented. A microfluidic device is fabricated with an elastomer substrate, polydimethylsiloxane (PDMS), under standard soft lithography. The performance of the microdevice is high-throughput, sensitive, and user-friendly. A total of 53.1% of the triangular microwells were able to trap single canine cutaneous mast cell tumor (MCT) cells. Of these, 38.82% were single cell entrapments, while 14.34% were multiple cell entrapments. The ratio of single-to-multiple cell trapping was high, at 2.7:1. In addition, 80.5% of the trapped cells were viable, indicating that the system was non-lethal. OCT4A-immunofluorescence combined with the proposed system can assess OCT4A expression in trapped single cells more precisely than OCT4A-immunohistochemistry. Therefore, the results suggest that microfluidic single cell analysis could potentially reduce the impact of cellular heterogeneity.

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“…Some microfluidic chips have been reported to capture single cells and to determine the impedance of the cells or circulating tumor cells. Furthermore, a microfluidic chip to trap single cells and to measure the impedance on cell membranes is demonstrated [12]. Recently, as represented in literature [13], a microfluidic technique capable of probing single cells is introduced.…”

Section: Introductionmentioning

confidence: 99%

Drug Discovery Acceleration Using Digital Microfluidic Biochip Architecture and Computer-aided-design Flow

Momtahen

Taajobian

Jahanian

2019

IJE

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A Digital Microfluidic Biochip (DMFB) offers a promising platform for medical diagnostics, DNA sequencing, Polymerase Chain Reaction (PCR), and drug discovery and development. Conventional Drug discovery procedures require timely and costly manned experiments with a high degree of human errors with no guarantee of success. On the other hand, DMFB can be a great solution for miniaturization, integration, automation, and cost reduction of drug discovery. DMFB can improve the parallelism of drug discovery procedures; since most procedures in drug discovery are concurrent and parallel, DMB can reduce the execution time of these bioassays. Therefore, there is a critical need to develop DMFBs to speed up the drug discovery applications and improve cost and error of these reactions. In this paper, a new architecture is used for drug discovery applications. The architecture is evaluated and compared with FPPC architecture. The experimental results prove that the new architecture is faster and cheaper than FPPC; it reduces all the important parameters such as total execution time, number of controlling pins, CAD algorithm execution time, and the area usage and its costs. There is an urgent need for collaboration between experts of drug discovery, microfluidic platform architecture and also machine learning to design a data-driven microfluidic architecture which improves the CAD algorithms by learning from prior knowledge.

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Digital Microfluidics for Manipulation and Analysis of a Single Cell

Cited by 59 publications

References 102 publications

Optofluidics 2015

Optofluidics 2015

A Potential Application of Triangular Microwells to Entrap Single Cancer Cells: A Canine Cutaneous Mast Cell Tumor Model

Drug Discovery Acceleration Using Digital Microfluidic Biochip Architecture and Computer-aided-design Flow

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